A5029666084- RNA modifications and cancer
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Sarcoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Gastric Cancer Management and Outcomes
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- Lung Cancer Treatments and Mutations
- Epilepsy research and treatment
- Chromatin Remodeling and Cancer
- Colorectal Cancer Treatments and Studies
- Liver Disease Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Organ Transplantation Techniques and Outcomes
- Wnt/β-catenin signaling in development and cancer
- Heat shock proteins research
Saiseikai Kumamoto Hospital
2014-2024
The University of Tokyo
2013-2023
Japan Agency for Marine-Earth Science and Technology
2020-2023
Tokushima University
2018-2022
Tokyo University of Science
2014-2021
Okayama University
2008-2020
Toyooka Hospital
2020
Kyushu Kyoritsu University
2020
Tohoku University
2017-2018
Kumamoto University
2014-2018
Targeted blockade of PD-1 with immune checkpoint inhibitors can activate T cells to destroy tumors. is believed function mainly at the effector, but not in activation, phase cell responses, yet how restricted activation stage currently unknown. Here we demonstrate that CD80 interacts PD-L1 cis on antigen-presenting (APCs) disrupt PD-L1/PD-1 binding. Subsequently, cannot engage inhibit when APCs express substantial amounts CD80. In knock-in mice which cis-PD-L1/CD80 interactions do occur,...
Abstract Aberrant methylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis lung cancers. There are major smoke exposure, histology, geography and gender‐related changes in non‐small cell cancer (NSCLC). We investigated smoking‐related, histologic, geographic gender differences profiles resected NSCLCs. examined 514 cases NSCLC 84 corresponding nonmalignant tissues from 4 countries (USA, Australia, Japan Taiwan) for status 7 to be methylated...
DNAs from 21 human stomach cancers, 16 metastatic cancers to lymph nodes, and apparently noncancerous specimens of mucosae a total 26 patients with cancer were tested for their ability induce neoplastic transformation NIH 3T3 cells on transfection by the calcium phosphate precipitation technique. Three samples DNA shown have transforming activity; one was primary patient, second portion mucosa same third node metastasis another patient. These transformants tumorigenic in nude mice, could...
Cancer-immunotherapy targeting programmed cell death 1 (PD-1) activates tumor-specific T cells and provides clinical benefits in various cancers. However, the molecular basis of PD-1 function is still enigmatic. Especially, it unclear which signaling pathway primarily targets. Besides, capacity to inhibit receptor (TCR)-dependent activation presence co-stimulation also controversial. Here we used co-culture systems antigen-presenting with targeted deletion overexpression co-receptors ligands...
Abstract Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI) have been strikingly effective in lung cancers harboring activating EGFR mutations. Unfortunately, the cancer cells eventually acquire resistance to EGFR-TKI. Approximately 50% of acquired involves a secondary T790M mutation. To overcome resistance, we focused on suppression using microRNA-7 (miR-7), targeting multiple sites 3′-untranslated region mRNA. Two EGFR-TKI–sensitive cell lines (PC-9 and H3255) two...
IL-1R antagonist-deficient (Il1rn(-/-)) mice develop autoimmune arthritis in which IL-17A plays a crucial role. Although many studies have shown that Th17 cell differentiation is dependent on TGF-β and IL-6, we found cells developed normally Il1rn(-/-)Il6(-/-) vivo. Then, analyzed the mechanisms of mice. We IL-21 production was increased lymph nodes Il1rn(-/-) mice, naive Il6(-/-) CD4(+) T differentiated into when cultured with IL-21, greatly enhanced IL-1 added to culture. not induced by...
Abstract Interleukin-17 (IL-17)-producing γδ T (γδ17) cells have been implicated in inflammatory diseases, but the underlying pathogenic mechanisms remain unclear. Here, we show that both CD4 + and γδ17 are required for development of autoimmune arthritis IL-1 receptor antagonist (IL-1Ra)-deficient mice. Specifically, activated direct T-cell infiltration by inducing CCL2 expression joints. Furthermore, IL-17 reporter mice reveal Vγ6 subset CCR2 preferentially produces inflamed Importantly,...
In gastric cancer, lymph node metastasis is one of the major prognostic factors and forms basis for surgical removal local nodes. Recently, several studies have demonstrated that overexpression lymphangiogenic growth factor VEGF‐C or VEGF‐D induces tumor lymphangiogenesis promotes lymphatic in mouse models. We examined whether these processes could be inhibited naturally metastatic tumors by blocking their cognate receptor VEGFR‐3 signaling pathway. Using a orthotopic cancer model which has...
We analyzed MET protein and copy number in NSCLC with or without EGFR mutations untreated tyrosine kinase inhibitors (TKIs). was examined 28 4 human bronchial epithelial cell lines (HBEC) 100 primary tumors using quantitative real-time PCR. Positive results were confirmed by array comparative genomic hybridization fluorescence in-situ hybridization. Total phospho-MET expression determined 24 2 HBEC Western blot. for exon 19 deletions, T790M, L858R. Knockdown of siRNA performed to examine the...
Loss of heterozygosity (LOH) has been frequently detected at chromosome 7q31 region in human head and neck squamous cell carcinomas (HNSCC) many other cancers, suggesting the existence tumor suppressor genes (TSG). We analysed LOH 49 HNSCC by using six polymorphic microsatellite markers found allelic deletion 48% (22/46) informative cases. two preferentially deleted regions, one is around D7S643 D7S486. When we redefined map according to contiguous sequences, a recently identified gene,...