A5004335610- Spinal Cord Injury Research
- Nerve injury and regeneration
- Respiratory Support and Mechanisms
- Education, Psychology, and Social Research
- Respiratory and Cough-Related Research
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Asthma and respiratory diseases
- Diabetic Foot Ulcer Assessment and Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Eastern European Communism and Reforms
- Cardiac Arrest and Resuscitation
- Mechanical Circulatory Support Devices
- Dialysis and Renal Disease Management
- Thermal Regulation in Medicine
- Neurogenetic and Muscular Disorders Research
- Sinusitis and nasal conditions
- Sulfur Compounds in Biology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- DNA Repair Mechanisms
- Mast cells and histamine
- Pressure Ulcer Prevention and Management
- Electrospun Nanofibers in Biomedical Applications
- Zebrafish Biomedical Research Applications
Quantum Genomics (France)
2023-2025
Charles University
2010-2023
Pilsen Region
2020-2023
Pilsen Tools (Czechia)
2023
Institute of Clinical and Experimental Medicine
2017-2022
Czech Academy of Sciences
2011-2022
Czech Academy of Sciences, Institute of Experimental Medicine
2013-2022
Czech Academy of Sciences, Institute of Philosophy
2015-2022
Palacký University Olomouc
1967-2020
Karlovy Vary Region
2020
Transplantation of mesenchymal stem cells (MSC) improves functional recovery in experimental models spinal cord injury (SCI); however, the mechanisms underlying this effect are not completely understood. We investigated intrathecal implantation human MSC on recovery, astrogliosis and levels inflammatory cytokines rats using balloon-induced compression lesions. Transplanted did survive at lesion site cord; was enhanced MSC-treated group as confirmed by Basso, Beattie, Bresnahan (BBB) flat...
Three different sources of human stem cells—bone marrow-derived mesenchymal cells (BM-MSCs), neural progenitors (NPs) derived from immortalized spinal fetal cell line (SPC-01), and induced pluripotent (iPSCs)—were compared in the treatment a balloon-induced cord compression lesion rats. One week after lesioning, rats received either BM-MSCs (intrathecally) or NPs (SPC-01 iPSC-NPs, both intraspinally), saline. The were assessed for their locomotor skills (BBB, flat beam test, rotarod)....
Abstract Background Stem cell treatment provides a promising therapy for patients with spinal cord injury (SCI). However, the applied stem cells exert their effects in different manners that are dependent on route used administration. Methods In present study, we administered neural precursors derived from induced pluripotent (iPS-NPs) either intraspinally into lesion center or intrathecally subarachnoid space of rats balloon-induced compression lesion. Functional locomotor performance,...
Mesenchymal stromal cells attract much interest in tissue regeneration because of their capacity to differentiate into mesodermal origin cells, paracrine properties and possible use autologous transplantations. The aim this study was investigate the safety reparative potential implanted human mesenchymal (hMSCs), prepared under Good Manufacturing Practice (GMP) conditions utilizing mixed platelet lysate as a culture supplement, collagenase Achilles tendon injury model rats. Eighty-one rats...
Well known for its anti-oxidative and anti-inflammation properties, curcumin is a polyphenol found in the rhizome of Curcuma longa. In this study, we evaluated effects on behavioral recovery, glial scar formation, tissue preservation, axonal sprouting, inflammation after spinal cord injury (SCI) male Wistar rats. The rats were randomized into two groups following balloon compression at level T9–T10 cord, namely vehicle- or curcumin-treated. Curcumin was applied locally surface injured...
Human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) were used for the treatment of ischemic-compression model spinal cord injury in rats. To assess effectivity treatment, different dosages (0.5 or 1.5 million cells) and repeated applications compared. Cells saline applied intrathecally by lumbar puncture one week only, three consecutive weeks after injury. Rats assessed locomotor skills (BBB, rotarod, flat beam) 9 weeks. Spinal tissue was morphometrically analyzed axonal...
Spinal cord injury (SCI) is a debilitating condition which characterized by an extended secondary due to the presence of inflammatory local milieu. Epigallocatechin gallate (EGCG) appears possess strong neuroprotective properties. Here, we evaluated beneficial effect EGCG on recovery from SCI. Male Wistar rats were given either or saline directly injured spinal and thereafter daily IP injection. Behavior was monitored BBB, plantar, rotarod flat-beam tests. The levels cytokines determined...
Abstract Background The growing use of whole exome/genome sequencing for diagnosing hereditary diseases has increased the interpretive workload clinical laboratories. Efficient methods are needed to identify pathogenic variants and maximize diagnostic yield without overwhelming resources. Methods We developed DiagAI, an AI-powered system trained on 2.5 million ClinVar predict ACMG pathogenicity classes. DiagAI ranks variants, proposes shortlists, identifies probands likely receive molecular...
The transplantation of stem cells may have a therapeutic effect on the pathogenesis and progression neurodegenerative disorders. In present study, we transplanted human mesenchymal (MSCs) into lateral ventricle triple transgenic mouse model Alzheimer's disease (3xTg-AD) at age eight months. We evaluated spatial reference working memory after MSC treatment possible underlying mechanisms, such as influence MSCs neurogenesis in subventricular zone (SVZ) expression levels 56 kDa oligomer amyloid...
Spinal cord injury (SCI), is a devastating condition leading to the loss of locomotor and sensory function below injured segment. Despite some progress in acute SCI treatment using stem cells biomaterials, chronic remains be addressed. We have assessed use laminin-coated hydrogel with dual porosity, seeded induced pluripotent cell-derived neural progenitors (iPSC-NPs), rat model SCI. iPSC-NPs cultured for 3 weeks vitro were positive nestin, glial fibrillary acidic protein (GFAP)...
Abstract Perineuronal nets (PNNs) enwrap mature neurons, playing a role in the control of plasticity and synapse dynamics. PNNs have been shown to effects on memory formation, retention extinction variety animal models. It has proposed that cavities PNNs, which contain synapses, can act as store they remain stable after events cause synaptic withdrawal such anoxia or hibernation. We examine this idea by monitoring place before caused acute hibernation-like state (HLS). Animals lacking...
Selective incircuit blood cooling could be an effective anticoagulation strategy during hemodialysis. However, it is currently unknown what temperature would ensure sufficient anticoagulation. Similarly, no information exists about potential interindividual variability in response to graded hypothermia. Therefore, the aim of this study was analyze effects profound hypothermia on human coagulation. Furthermore, a mathematical relationship between temperatures and coagulation sought predict...
Systematic inflammatory response after spinal cord injury (SCI) is one of the factors leading to lesion development and a profound degree functional loss. Anti-inflammatory compounds, such as curcumin epigallocatechin gallate (EGCG) are known for their neuroprotective effects. In this study, we investigated effect combined therapy EGCG in rat model acute SCI induced by balloon compression. Immediately SCI, rats received curcumin, EGCG, + or saline [daily intraperitoneal doses (curcumin, 6...
Methacrylate hydrogels have been extensively used as bridging scaffolds in experimental spinal cord injury (SCI) research. As synthetic materials, they can be modified, which leads to improved of the lesion. Fibronectin, a glycoprotein extracellular matrix produced by reactive astrocytes after SCI, is known promote cell adhesion. We implanted 3 methacrylate hydrogels: scaffold based on hydroxypropylmethacrylamid (HPMA), 2-hydroxyethylmethacrylate (HEMA) and HEMA hydrogel with an attached...
Background. A number of haemodialysis studies have demonstrated beneficial effects cooler dialysates on global haemodynamics in chronic dialysis patients. However, the continuous venovenous haemofiltration (CVVH)-induced cooling regional perfusion and energy metabolism critically ill septic patients not been well defined.
Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A combination biomaterials and stem cell therapy seems to be a promising approach increase regenerative potential after SCI. We evaluated use cellpolymer construct based on conditionally immortalized progenitor line SPC-01_GFP3, derived from human fetal tissue, with serotonin-modified poly(2-hydroxyethyl methacrylate) hydrogel (pHEMA-5HT). compared effect pHEMA-5HT seeded SPC-01_GFP3 cells, only...
Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A suitable combination modern hydrogel materials, modified to effectively bridge lesion cavity, combined with appropriate stem cell therapy seems be a promising approach repair damage. We demonstrate synergic effect porosity and surface modification hydrogels on mesenchymal (MSC) adhesiveness in vitro their vivo survival an experimental model SCI. MSCs were seeded four different hydrogels:...
Spinal cord injury leads to a robust inflammatory response that is an unfavorable environment for stem cell implantation. In this study, we evaluated the effect of combined therapy curcumin and mesenchymal cells (MSC) on behavioral recovery tissue sparing, glial scar formation, axonal sprouting responses in rat experimental model spinal (SCI). Balloon-induced compression lesion was performed at thoracic (Th8-9) level. Out four groups studied, two received surface immediately after SCI then...
Abstract We investigated the effect of a Multiwave Locked System laser (with simultaneous 808 nm continuous emission and 905 pulse emission) on spinal cord after injury (SCI) in rats. The functional recovery was measured by locomotor tests (BBB, Beam walking, MotoRater) sensitivity test (Plantar test). showed significant improvement functions rats treatment from first week following lesioning, compared to controls. significantly diminished thermal hyperalgesia SCI as Plantar test. atrophy...