A5002852942- Nerve injury and regeneration
- Mesenchymal stem cell research
- Spinal Cord Injury Research
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Wound Healing and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Burn Injury Management and Outcomes
- Plasma Applications and Diagnostics
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
Charles University
2016-2018
Czech Academy of Sciences, Institute of Experimental Medicine
2016-2018
Czech Academy of Sciences
2018
Restoration of lost neuronal function after spinal cord injury (SCI) still remains a big challenge for current medicine. One important repair strategy is bridging the SCI lesion with supportive and stimulatory milieu that would enable axonal rewiring. Injectable extracellular matrix (ECM)-derived hydrogels have been recently reported to neurotrophic potential in vitro. In this study, we evaluated presumed neuroregenerative properties ECM vivo acute model SCI. were prepared by...
Abstract Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of chronic wounds other skin pathologies. In this study we analysed effect NTP on healing full-thickness acute wound model in rats. We utilised a single jet system generating atmospheric pressure air plasma, with ion volume density 5 · 10 17 m −3 gas temperature 30–35 °C. The were exposed to three daily treatments 1 or 2 minutes evaluated 3, 7 14 days after...
Three different sources of human stem cells—bone marrow-derived mesenchymal cells (BM-MSCs), neural progenitors (NPs) derived from immortalized spinal fetal cell line (SPC-01), and induced pluripotent (iPSCs)—were compared in the treatment a balloon-induced cord compression lesion rats. One week after lesioning, rats received either BM-MSCs (intrathecally) or NPs (SPC-01 iPSC-NPs, both intraspinally), saline. The were assessed for their locomotor skills (BBB, flat beam test, rotarod)....
Abstract Hydrogel scaffolds which bridge the lesion, together with stem cell therapy represent a promising approach for spinal cord injury (SCI) repair. In this study, hydroxyphenyl derivative of hyaluronic acid (HA‐PH) was modified integrin‐binding peptide arginine‐glycine‐aspartic (RGD), and enzymatically crosslinked to obtain soft injectable hydrogel. Moreover, addition fibrinogen used enhance proliferation human Wharton's jelly‐derived mesenchymal cells (hWJ‐MSCs) on HA‐PH‐RGD The...
Human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) were used for the treatment of ischemic-compression model spinal cord injury in rats. To assess effectivity treatment, different dosages (0.5 or 1.5 million cells) and repeated applications compared. Cells saline applied intrathecally by lumbar puncture one week only, three consecutive weeks after injury. Rats assessed locomotor skills (BBB, rotarod, flat beam) 9 weeks. Spinal tissue was morphometrically analyzed axonal...
Systematic inflammatory response after spinal cord injury (SCI) is one of the factors leading to lesion development and a profound degree functional loss. Anti-inflammatory compounds, such as curcumin epigallocatechin gallate (EGCG) are known for their neuroprotective effects. In this study, we investigated effect combined therapy EGCG in rat model acute SCI induced by balloon compression. Immediately SCI, rats received curcumin, EGCG, + or saline [daily intraperitoneal doses (curcumin, 6...