A5076663431- Intracranial Aneurysms: Treatment and Complications
- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrovascular and Carotid Artery Diseases
- Spinal Cord Injury Research
- Nerve injury and regeneration
- Neurosurgical Procedures and Complications
- Vascular Malformations Diagnosis and Treatment
- Advanced Neuroimaging Techniques and Applications
- Cerebrospinal fluid and hydrocephalus
- Acute Ischemic Stroke Management
- Electrospun Nanofibers in Biomedical Applications
- Spinal Dysraphism and Malformations
- Meningioma and schwannoma management
- Moyamoya disease diagnosis and treatment
- Takotsubo Cardiomyopathy and Associated Phenomena
- Advanced MRI Techniques and Applications
- Nerve Injury and Rehabilitation
- Intracerebral and Subarachnoid Hemorrhage Research
- Mesenchymal stem cell research
- Ion channel regulation and function
- Neurogenetic and Muscular Disorders Research
- Anesthesia and Pain Management
- Cardiovascular Health and Disease Prevention
- Pain Management and Opioid Use
- Sepsis Diagnosis and Treatment
Czech Academy of Sciences, Institute of Experimental Medicine
2011-2024
St. Anne's University Hospital Brno
2014-2024
University Hospital Brno
2014-2024
Jan Evangelista Purkyně University in Ústí nad Labem
2010-2024
Krajská Zdravotní
2007-2022
Charles University
2006-2019
Czech Academy of Sciences
2009-2019
Mayo Clinic
2019
Military University Hospital Prague
2015-2019
University of Verona
2011-2019
Chronic spinal cord injury (SCI) is characterized by tissue loss and a stable functional deficit. While several experimental therapies have proven to be partly successful for the treatment of acute SCI, chronic SCI still challenging. We studied whether we can bridge lesion implantation our newly developed hydrogel based on 2-hydroxypropyl methacrylamide, either alone or seeded with mesenchymal stem cells (MSCs), this leads improvement. A balloon-induced compression was performed in adult...
The architecture and mechanical properties of a scaffold for spinal cord injury treatment must provide tissue integration as well effective axonal regeneration. Previous work has demonstrated the cell-adhesive growth-promoting SIKVAV (Ser–Ile–Lys–Val–Ala–Val)-modified highly superporous poly(2-hydroxethyl methacrylate) (PHEMA) hydrogels. aim current study was to optimize porosity this type hydrogel in order develop suitable repair tissue. Three types PHEMA hydrogels with oriented pores ~60...
Abstract Hydrogel scaffolds which bridge the lesion, together with stem cell therapy represent a promising approach for spinal cord injury (SCI) repair. In this study, hydroxyphenyl derivative of hyaluronic acid (HA‐PH) was modified integrin‐binding peptide arginine‐glycine‐aspartic (RGD), and enzymatically crosslinked to obtain soft injectable hydrogel. Moreover, addition fibrinogen used enhance proliferation human Wharton's jelly‐derived mesenchymal cells (hWJ‐MSCs) on HA‐PH‐RGD The...
Object Hydrogels are nontoxic, chemically inert synthetic polymers with a high water content and large surface area that provide mechanical support for cells axons when implanted into spinal cord tissue. Methods Macroporous hydrogels based on 2-hydroxyethyl methacrylate (HEMA) were prepared by radical copolymerization of monomers in the presence fractionated NaCl particles. Male Wistar rats underwent complete transection at T-9 level. To bridge lesion, positively charged HEMA either...
Spinal cord injury (SCI), is a devastating condition leading to the loss of locomotor and sensory function below injured segment. Despite some progress in acute SCI treatment using stem cells biomaterials, chronic remains be addressed. We have assessed use laminin-coated hydrogel with dual porosity, seeded induced pluripotent cell-derived neural progenitors (iPSC-NPs), rat model SCI. iPSC-NPs cultured for 3 weeks vitro were positive nestin, glial fibrillary acidic protein (GFAP)...
Diffusion tensor imaging (DTI) is now widely used in neurosurgery to preoperatively delineate the course of pyramidal tract.To evaluate accuracy method by comparison with subcortical electrical stimulation and influence distance tract from tumor on resection extent postoperative clinical deficits.A diffusion depiction was preoperative planning intraoperative navigation 72 cases. In 36 cases, during resection. The tumor-to-tract compared result, resection, short-term course.A significant...
Abstract Modifications of poly(2‐hydroxyethyl methacrylate) (PHEMA) with cholesterol and the introduction large pores have been developed to create highly superporous hydrogels that promote cell–surface interactions can serve as a permissive scaffold for spinal cord injury (SCI) treatment. Highly cholesterol‐modified PHEMA scaffolds prepared by bulk radical copolymerization 2‐hydroxyethyl methacrylate (HEMA), (CHLMA), ethylene dimethacrylate (EDMA) cross‐linking agent in presence ammonium...
Methacrylate hydrogels have been extensively used as bridging scaffolds in experimental spinal cord injury (SCI) research. As synthetic materials, they can be modified, which leads to improved of the lesion. Fibronectin, a glycoprotein extracellular matrix produced by reactive astrocytes after SCI, is known promote cell adhesion. We implanted 3 methacrylate hydrogels: scaffold based on hydroxypropylmethacrylamid (HPMA), 2-hydroxyethylmethacrylate (HEMA) and HEMA hydrogel with an attached...
Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A combination biomaterials and stem cell therapy seems to be a promising approach increase regenerative potential after SCI. We evaluated use cellpolymer construct based on conditionally immortalized progenitor line SPC-01_GFP3, derived from human fetal tissue, with serotonin-modified poly(2-hydroxyethyl methacrylate) hydrogel (pHEMA-5HT). compared effect pHEMA-5HT seeded SPC-01_GFP3 cells, only...
Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A suitable combination modern hydrogel materials, modified to effectively bridge lesion cavity, combined with appropriate stem cell therapy seems be a promising approach repair damage. We demonstrate synergic effect porosity and surface modification hydrogels on mesenchymal (MSC) adhesiveness in vitro their vivo survival an experimental model SCI. MSCs were seeded four different hydrogels:...
<h3>BACKGROUND AND PURPOSE:</h3> MRA is widely accepted as a noninvasive diagnostic tool for the detection of intracranial aneurysms, but still challenging task with rather low rates. Our aim was to examine performance computer-aided diagnosis algorithm detecting aneurysms on in clinical setting. <h3>MATERIALS METHODS:</h3> Aneurysm detectability evaluated retrospectively 48 subjects and without by 6 readers using 3D viewing system. Aneurysms ranged from 1.1 6.0 mm (mean = 3.12 mm, median...
Anesthetics can either promote or inhibit the development of neurogenic pulmonary edema (NPE) after central nervous system (CNS) injury. The influence isoflurane was examined in male Wistar rats using 1.5%, 2%, 2.5%, 3%, 4%, 5% air. Epidural balloon compression thoracic spinal cord performed. NPE vivo and on histologic sections lung tissue. Animals anesthetized with 1.5% 3% were behaviorally monitored BBB plantar tests for 7 weeks post-injury. MRI morphometry spared white gray matter. All...
Computational fluid dynamics (CFD) has grown as a tool to help understand the hemodynamic properties related rupture of cerebral aneurysms. Few these studies deal specifically with aneurysm growth and most only use single time instance within history. The present retrospective study investigated four patient-specific aneurysms, once at initial diagnosis then follow-up, analyze morphological changes. Aneurysm geometries were segmented via medical image processing software Mimics. meshed...