A5052761981- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Animal Behavior and Welfare Studies
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Enterobacteriaceae and Cronobacter Research
- Reproductive tract infections research
- Vibrio bacteria research studies
- Microbial Metabolism and Applications
- COVID-19 Clinical Research Studies
- Animal Nutrition and Physiology
- Viral-associated cancers and disorders
- Molecular Biology Techniques and Applications
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
Vrije Universiteit Brussel
2020-2024
Universitair Ziekenhuis Brussel
2020-2024
Liverpool School of Tropical Medicine
2016-2023
Huisarts en Wetenschap
2021
Veterinary and Agrochemical Research Centre
2011-2015
Ghent University
2011-2015
Instituut voor Landbouw en Visserijonderzoek
2012
From the mid-2000s on, numerous studies have shown that methicillin-resistant Staphylococcus aureus (MRSA), renowned as human pathogen, has a reservoir in pigs and other livestock. In Europe North America, clonal complex (CC) 398 appears to be predominant lineage involved. Especially worrisome is its capacity contaminate humans close contact with affected animals. Indeed, typical multi-resistant phenotype of MRSA CC398 observed ability easily acquiring genetic material suggests strains an...
Abstract Whole genome sequencing (WGS) enables detailed characterization of bacteria at single nucleotide resolution. It provides data about acquired resistance genes and mutations leading to resistance. Although WGS is becoming an essential tool predict patterns accurately, comparing genotype phenotype with still in its infancy. Additional validation are needed. In this retrospective study, we analysed 234 E. coli isolates from positive blood cultures using as well microdilution for 11...
The prevalence, distribution, and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) in Belgian pig farms has been investigated. To that end, nasal samples were collected from 1,500 pigs on 50 randomly selected over Belgium. Both closed (breeding or farrow-to-finish) open (fattening) included. Within different age groups A total number 663 (44%) belonging to 34 (68%) carried MRSA. According their management practice, MRSA was detected 94% the 56% farms....
Whole genome sequencing (WGS) enables complete characterization of bacterial pathogenic isolates at single nucleotide resolution, making it the ultimate tool for routine surveillance and outbreak investigation. The lack standardization, variation regarding bioinformatics workflows parameters, however, complicates interoperability among (inter)national laboratories. We present a validation strategy applied to workflow Illumina data that performs Shiga toxin-producing Escherichia coli (STEC)...
During the last few years, methicillin-resistant Staphylococcus aureus (MRSA) ST398 has been isolated frequently from livestock, especially pigs and to a lesser extent cattle poultry. To gain insight into distribution of this bacterium in pig farms versus multispecies farms, 30 Belgian (10 pig, 10 pig/poultry pig/cattle farms) were screened for presence MRSA. On each farm, nasal swabs taken pigs. When present, (n=10) sampled nares poultry nares, earlobes cloaca. A selection obtained isolates...
This study aimed at investigating bovine non-Staphylococcus aureus staphylococci for their role as a potential reservoir methicillin resistance. Nasal swab samples were collected from 150 veal calves on 15 farms, 100 dairy cows 10 farms and beef farms. Suspected staphylococcal isolates investigated by PCR the presence of classic mecA mecALGA251. Methicillin-resistant non-S. (MRNAS) genotypically identified characterized broth microdilution antimicrobial susceptibility testing cassette...
Malaria hotspots, areas with consistently higher than average transmission, may become increasingly common as malaria declines. This phenomenon, currently observed in The Gambia, be caused by several factors, including some related to the local vectors, whose contribution is poorly understood. Using WHO susceptibility bioassays, insecticide resistance status was determined vector populations sampled from six pairs of villages across each pair contained a low and high prevalence village....
Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, National Reference Laboratories of smaller countries often show slower uptake WGS, mainly because significant investments required to generate and analyze data a limited amount samples. To facilitate this process incite policy makers support its implementation, Shiga toxin-producing Escherichia coli (STEC) O157:H7 (stx1+, stx2+, eae+)...
BackgroundHospital-acquired infections caused by VIM-encoded metallo-β-lactamase-positive Pseudomonas aeruginosa are a major problem in intensive care units (ICUs) worldwide. A previous study conducted the UZ Brussel hospital revealed that sink drains of ICU were possible source various multidrug-resistant pathogenic bacteria.AimTo investigate presence and persistence VIM P. four adult ICUs their role nosocomial infections, emphasizing sink-to-patient transmission.MethodsThirty-six sinks...
It is generally accepted that microorganisms can colonize a non-pathological endometrium. However, in clinical setting, endometrial samples are always collected by passing through the vaginal-cervical route. As such, vaginal and cervical microbiomes easily cross-contaminate samples, resulting biased representation of microbiome. This makes it difficult to demonstrate microbiome not merely reflection contamination originating from sampling. Therefore, we investigated what extent corresponds...
Escherichia coli O157 is a Shiga toxin–producing E. causing disease in humans. Cattle are the primary reservoir of pathogen. Information regarding contribution cattle to diarrheal illnesses humans through consumption contaminated beef scarce Ethiopia. We collected samples from 240 cattle, 127 beef, and 216 diarrheic patients Bishoftu town Ethiopia assess occurrence determine virulence genes, genetic relatedness, antimicrobial resistance O157. was detected 7.1% rectal content slaughterhouses,...
A transmission experiment was performed to evaluate the spread of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in nursery piglets. Reproduction ratios (R(0)) three experimental groups were found vary between 3.92 and 52.54, indicating that after introduction, MRSA will easily among weaned piglets, with a tendency become established.
The purpose of this work was to identify and genetically characterize enterohemorrhagic Escherichia coli (EHEC) enteropathogenic E. (EPEC) O80:H2 from diarrhoeic septicaemic calves in Belgium comparing them with human EHEC after whole genome sequencing. Ten 21 EPEC O80 identified by PCR between 2009 2018 faeces, intestinal content a kidney or were sequenced compared 19 2008 2019. They all belonged the serotype ST301, harboured eaeξ gene, 23 29 contained stx2d gene. Phylogenetically, they...
The microbiome of the reproductive tract has been associated with (sub)fertility and it suggested that dysbiosis reduces success rates pregnancy outcomes. endometrial is particular interest given potential impact on embryo implantation. To date, all studies have applied a metagenomics approach. A sequencing-based technique, however, its limitations, more specifically in adequately exploring low-biomass settings, such as intra-uterine/endometrial samples. In this proof-of-concept study, we...
Abstract Background Salmonella and E. coli O157 are common causes of foodborne diseases. Evisceration de-hiding steps can lead to carcass contamination during slaughter operation. In Ethiopia, information on the association between presence these pathogens in rectal content and/or hide cattle their is lacking. Methods The aim this study was assess sources beef with slaughter. Rectal contents hide- carcass-swabs (from three sites: foreleg, brisket hind leg) were collected from 70 at two small...
Shiga toxin-producing Escherichia coli (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise outbreak prevention and confinement, broadening epidemiology contributing to risk assessment source attribution. However, despite international recommendations, WGS often restricted assist investigation not yet fully implemented food safety across all European countries, contrast for example the United States.In this study,...