Phytoestrogens, polyphenolic compounds derived from plants, are more and common constituents of human animal diets. In most the cases, these chemicals much less potent than endogenous estrogens but exert their biological effects via similar mechanisms action. The source phytoestrogen exposure to humans as well ruminants is soybean-derived foods that rich in isoflavones genistein daidzein being metabolized digestive tract even metabolites— para -ethyl-phenol equol. Phytoestrogens have...

The aim of the study was to assess granulocyte colony-stimulating factor (G-CSF) effects on unresponsive thin (<7 mm) endometrium in women undergoing vitro fertilization (IVF). We included thirty-seven subjects who had day triggering ovulation. These patients also failed achieve an adequate endometrial thickness at least one their previous IVF cycles. In all time infusion G-CSF, 6,74 ± 1,75 mm, and, after infusion, it increased significantly 8,42 1,73 mm. When we divided group into two...

Background: The introduction of the vaccination against SARS-CoV-2 infection creates need for precise tools quality control procedures, detection poor humoral response, and estimation achieved protection disease. Thus, study aimed to compare results anti-SARS-CoV-2 tests evaluate application WHO standard unitage (the binding antibody units; BAU/mL) a measurement response vaccination. Methods: Patients undergoing with Pfizer/BioNTech BNT162b2 (BNT162b2) (n = 79), referred prior 21 days after...

Progesterone is suggested to be a suppressor of apoptosis in bovine luteal cells. Fas antigen (Fas) cell surface receptor that triggers sensitive Furthermore, known controlled by the bcl-2 gene/protein family and caspases. This study was undertaken determine whether intraluteal progesterone (P4) involved L-mediated death corpus luteum (CL) vitro. Moreover, we studied an antagonist P4 influences gene expression caspase-3 activity CL. Luteal cells obtained from cows midluteal phase estrous...

In the cow, lysophosphatidic acid (LPA) acts as an auto-/paracrine factor, through its receptors LPAR1-4, on oocytes and cumulus cells during in vitro maturation (IVM). The aim of present work was to determine effect LPA IVM bovine on: 1) oocyte maturation; 2) apoptosis COCs; 3) expression genes involved developmental competence subsequent blastocysts; 4) expansion ovulatory cascade cells; 5) glucose metabolism utilization 6) cleavage blastocyst rates Day 2 7 culture, respectively....

Abstract Embryo implantation in the mare occurs just over one month after fertilization, coinciding with production of chorionic gonadotropin. The factors that regulate this late mare, and whether they are unique to horses or shared more invasive embryo other species, remain poorly understood. This study aimed determine compare transcriptome subpopulations endometrial cells before horse. Single-cell RNA sequencing was used characterize nearly 97,000 collected from biopsies endometrium at...

Phytoestrogens acting as endocrine disruptors may induce various pathologies in the female reproductive tract. The purpose of this study was to determine whether phytoestrogens present soybean and/or their metabolites are detectable plasma cows fed a diet rich soy and these influence efficiency prostaglandin (PG) synthesis during estrous cycle early pregnancy bovine endometrium. In vivo Experiment 1, we found significant levels daidzein genistein fodder (equol p-ethyl-phenol) serum urine....

We have suggested in a previous vitro study that tumor necrosis factor-α (TNFα) plays role the initiation of luteolysis cattle. The aim present was to examine influence different doses TNFα on estrous cycle cattle by observing standing behavior and measuring peripheral concentrations progesterone (P4) during cycle. Moreover, we evaluated secretion P4, oxytocin (OT), nitric oxide (NO), luteolytic (prostaglandin F2α [PGF2α] leukotriene C4 [LTC4]) luteotropic (PGE2) metabolites arachidonic acid...

We previously showed in vivo and vitro studies that nitric oxide (NO) is engaged luteolysis cattle. Nitric produced locally the bovine corpus luteum (CL) inhibits progesterone (P4) synthesis suggested to be a component of luteolytic cascade induced by uterine prostaglandin (PG) F2α. In present study, molecular mechanisms NO action during structural were studied cultured luteal cells (Days 15-17 estrous cycle). The effects donor (NONOate; 10-4M) on DNA fragmentation, cell viability, P4...

The aim of this study was to examine whether active metabolites phytoestrogens (equol and para-ethyl-phenol) inhibit sensitivity bovine corpus luteum (CL) luteinizing hormone (LH) auto/paracrine luteotropic factors (prostaglandin E2-PGE2 prostaglandin F2α-PGF2α), they influence pulsatile progesterone (P4) secretion by the CL. In in vivo experiments, high levels equol para-ethyl-phenol were found plasma CL tissue heifers cows fed a soy bean diet (2.5 kg/animal/day), along with lower...

Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 mice may lead to embryo mortality. In present study, we examined role bovine uterus. We confirmed that is locally produced and released from Moreover, there are enzymes involved (phospholipase (PL) D(2) PLA2G1B) endometrium during estrous cycle early pregnancy. Expression for (LPAR1) was positively correlated with expression PGE(2) synthase...

We have previously documented synthesis of lysophosphatidic acid (LPA) in the bovine endometrium and increased presence LPA receptor mRNA expression during pregnancy. Therefore, could contribute to early pregnancy establishment cow. In present study, we investigated effect intravaginally administered on rates plasma levels progesterone (P4) prostaglandins (PGs) heifers. Animals were inseminated from day 15 18 after estrus treated with saline, (1 mg) or blocker (VPC32183; 1 mg). Blood samples...

Lysophosphatidic acid (LPA) is a known cell signaling lipid mediator in reproductive tissues. In the cow, LPA involved luteal and early pregnancy maintenance. Here, we evaluated presence role of bovine embryonic development. relevant aspects, embryos reflect more closely scenario occurring human than mouse model. Transcription mRNA protein expression enzymes synthesis (ATX c PLA 2 ) receptors (LPAR1–4) were detected Days 5 8 vitro produced embryos. Embryonic production into culture medium...

We studied the secretory function of corpus luteum (CL) in cows following different estrus synchronization protocols. Estrus was synchronized using one (n=4) or two injections (n=5) prostaglandin F2α (PGF2α; dinoprost), analogues PGF2α (aPGF2α), luprostiol and cloprostenol (n=5), at eleven-day intervals, a gestagen implant (norgestomet, n=5, for 10 days) norgestomet together with subsequent dinoprost injection on day removal (n=5). CL samples were collected by ovariectomy Day 7-8 estrous...

Lysophosphatidic acid (LPA) has been shown to be a potent modulator of prostaglandin (PG) secretion during the luteal phase estrous cycle in bovine endometrium vivo. The aims present study were determine cell types (epithelial or stromal cells) responsible for PGs response LPA, cellular, receptor, intracellular, and enzymatic mechanisms LPA action. Cultured epithelial cells exposed (10 −5 –10 −9 M), tumor necrosis factor α (TNFα; 10 ng/mL) oxytocin (OT; −7 M) 24 h. treatment resulted...

We examined whether the CL is a site for lysophosphatidic acid (LPA) synthesis and/or target LPA action in bovine reproductive tract. concentrations tissue increased towards end of cycle and were stable during early pregnancy. No changes expression receptors (LPARs) occurred estrous cycle. The expressions LPAR2 LPAR4 on days 17–19 pregnancy higher than those respective 8–10 stimulated P4 via 3βHSD stimulation but did not modulate interferon–tau (IFNτ) influence steroidogenic cells. Moreover,...

In the present study we examined whether LPA can be synthesized and act during in vitro maturation of bovine cumulus oocyte complexes (COCs). We found transcription genes coding for enzymes synthesis pathway ( ATX PLA2 ) receptors LPAR 1–4 oocytes cells, following maturation. COCs were matured presence or absence (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...

The aim of study was to correlate tumour necrosis factor-alpha (TNF) infused doses used with the TNF concentrations achieved and secretory function both ovary uterus in cows. We evaluated progesterone (P4), prostaglandin (PG)F(2alpha), PGE(2) nitric oxide (NO) jugular vein vena cava caudalis as parameters exogenous action on female reproductive tract. Aortae abdominalis cows (n = 18) were saline or two (luteolytic--1 microg luteotrophic--10 microg). In peripheral blood, 1 within range 30-45...