A5028477575- Immune Cell Function and Interaction
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Mycobacterium research and diagnosis
- T-cell and B-cell Immunology
- Histone Deacetylase Inhibitors Research
- Amino Acid Enzymes and Metabolism
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Cancer Cells and Metastasis
- Inflammasome and immune disorders
- Renal cell carcinoma treatment
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Reproductive tract infections research
- Renal Diseases and Glomerulopathies
- PARP inhibition in cancer therapy
- Cervical Cancer and HPV Research
- Cytokine Signaling Pathways and Interactions
- Advanced Breast Cancer Therapies
- Cancer, Lipids, and Metabolism
- Cytomegalovirus and herpesvirus research
Louisiana State University Health Sciences Center New Orleans
2013-2024
Louisiana State University
1997-2024
Louisiana Cancer Research Center
2007-2021
Tulane University
1989-2018
University of North Carolina at Chapel Hill
2018
Stanley Foundation
2003-2013
Bipar
2013
Children's Hospital of New Orleans
2007
Institute of Neuroimmunology of the Slovak Academy of Sciences
2005
University Medical Center New Orleans
1998-2000
Abstract T cells infiltrating tumors have a decreased expression of signal transduction proteins, diminished ability to proliferate, and production cytokines. The mechanisms causing these changes remained unclear. We demonstrated recently that peritoneal macrophages stimulated with interleukin 4 + 13 produce arginase I, which decreases the T-cell receptor CD3ζ chain impairs responses. Using 3LL murine lung carcinoma model we tested whether I was produced in tumor microenvironment could...
Immune dysfunction reported in renal cell carcinoma (RCC) patients may contribute to tumor progression. Myeloid-derived suppressor cells (MDSC) represent one mechanism by which tumors induce T-cell suppression. Several factors pivotal the accumulation of MDSC are targeted tyrosine kinase inhibitor, sunitinib. The effect sunitinib on MDSC-mediated immunosuppression RCC has been investigated.Patient peripheral blood levels and regulatory (Treg) production IFN-gamma were evaluated before after...
Abstract Myeloid suppressor cells with high arginase activity are found in tumors and spleen of mice colon lung cancer. These cells, described as macrophages or immature dendritic deplete arginine impair T cell proliferation cytokine production. Although has been cancer patients, it is thought to originate from tumor metabolizing ornithine needed sustain rapid proliferation. The goal this study was determine whether myeloid producing existed renal carcinoma patients. Peripheral blood...
l-Arg plays a central role in the normal function of several organ systems including immune system. can be depleted by arginase I produced macrophages and hepatocytes disease states such as trauma sepsis following liver transplantation. The decrease inl-Arg levels induces profound T cell through mechanisms that have remained unclear. data presented here demonstrate Jurkat cells cultured medium withoutl-Arg (l-Arg-free RPMI) rapid expression antigen receptor ζ chain (CD3ζ), principal signal...
Abstract l-Arginine plays a central role in the normal function of several organs including immune system. It is metabolized macrophages by inducible nitric oxide synthase to produce oxide, important cytotoxic mechanisms, and arginase I (ASE I) II II) synthesize l-ornithine urea, first being precursor for production polyamines needed cell proliferation. availability can modulate T function. Human cells stimulated cultured absence l-arginine lose expression TCR ζ-chain (CD3ζ) have an impaired...
In vitro models of Chlamydia trachomatis growth have long been studied to predict in vivo. Alternative or persistent modes shown occur under the influence numerous stressors but not Here, we report development methods for sampling human infections from endocervix a manner that permits multifaceted analysis bacteria, host and endocervical environment. Our approach evaluating total bacterial load, transcriptional patterns, morphology by immunofluorescence electron microscopy, levels cytokines...
Abstract Helicobacter pylori infects approximately half the human population. The outcomes of infection range from gastritis to gastric cancer and appear be associated with immunity H. pylori. Patients developing nonatrophic present a Th1 response without protective immunity, suggesting that this bacterium may have mechanisms evade immune host. Several proteins can impair macrophage T cell function in vitro through are poorly understood. We tested effect extracts live on Jurkat cells freshly...
"Didelphis marsupialis, an Important Reservoir of Trypanosoma (Schizotrypanum) cruzi and Leishmania (Leishmania) chagasi in Colombia" published on May 1994 by The American Society Tropical Medicine Hygiene.
ABSTRACT Advanced stages of mycobacterial diseases such as leprosy and tuberculosis are characterized by a loss T-cell function. The basis this dysfunction is not well understood. present report demonstrates major alterations in the expression signal transduction molecules T cells patients. These were most frequently observed lepromatous (LL) Of 29 LL patients, 69% had decreased receptor ζ-chain expression, 48% p56 lck tyrosine kinase, 63% nuclear transcription factor NF-κB p65. An...
Background.The protective immune response against Mycobacterium tuberculosis relies both on antigenpresenting cells and T lymphocytes.In patients with different forms of tuberculosis, varying degrees cell function-ranging from positive delayed-type hypersensitivity, in asymptomatic infected healthy individuals, to the absence response, miliary or pulmonary (PTB)-have been reported.The decreased expression CD3z reported either cancer leprosy has provided possible explanations for altered...
ABSTRACT Gamma interferon (IFN-γ) induces expression of the tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase (IDO1) in human epithelial cells, permissive cells for obligate intracellular bacterium Chlamydia trachomatis . IDO1 depletes tryptophan by catabolizing it to kynurenine with consequences C. , which is a auxotroph. In vitro studies reveal that depletion can result formation persistent (viable but noncultivable) chlamydial forms. Here, we tested effects inhibitor,...
Abstract Polycyclic aromatic hydrocarbons (PAHs) are the products of incomplete combustion organic materials, which present in cigarette smoke, deep‐fried food, and natural crude oil. Since PAH‐metabolites form DNA adducts cause oxidative damage, we asked if these environmental carcinogens could affect transforming potential human Polyomavirus JC oncoprotein, T‐antigen (JCV T‐antigen). We extracted DMSO soluble PAHs from Deepwater Horizon oil spill Gulf Mexico (oil‐PAHs), detected several...
Antagonists to the N-methyl-D-aspartate (NMDA) receptor bind various extraneuronal tissues. We therefore assessed expression of main NMDA subunit, NR1, in demonstrate that NR1 appears be most abundant rat kidney and heart. is present total kidney, cortex, medulla. Of NR2 subunits, only NR2C subunit protein kidney. The abundance increases with development. Both are opossum Madin-Darby canine LLC-PK(1) cells. Immunohistochemistry studies show renal proximal tubule. atrium ventricle but also...
Our laboratory established a role for poly(ADP-ribose)polymerase (PARP) in asthma. To increase the clinical significance of our studies, it is imperative to demonstrate that PARP actually activated human asthma, examine whether inhibitor approved testing such as olaparib blocks already-established chronic asthma traits response house dust mite (HDM), true allergen, mice and drug modulates cluster differentiation type 4 (CD4+) T-cell function. conduct study, lung specimens peripheral blood...
Low selenium levels have been linked to a higher incidence of cancer and other diseases, including Keshan, Chagas, Kashin-Beck, insulin resistance. Additionally, muscle cardiovascular disorders, immune dysfunction, cancer, neurological endocrine function associated with mutations in genes encoding for selenoproteins. Selenium biology is complex, systems approach study global metabolomics, genomics, and/or proteomics may provide important clues examining selenium-responsive markers...