A5015517692- Prostate Cancer Treatment and Research
- Cell Adhesion Molecules Research
- Prostate Cancer Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Metabolomics and Mass Spectrometry Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- TGF-β signaling in diseases
- Hormonal and reproductive studies
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- Liver physiology and pathology
- Radiopharmaceutical Chemistry and Applications
- FOXO transcription factor regulation
- Angiogenesis and VEGF in Cancer
- Anesthesia and Neurotoxicity Research
- Protease and Inhibitor Mechanisms
- Cellular transport and secretion
- Caveolin-1 and cellular processes
University of Florida
2019-2023
Jacksonville College
2023
Armstrong Center for Medicine & Health
2022
Jacksonville University
2020
Louisiana State University Health Sciences Center New Orleans
2004-2019
Roswell Park Comprehensive Cancer Center
2011-2018
Cancer Genetics (United States)
2011-2018
University of Kentucky
2014
George Washington University
2012
Tulane University
2012
Accumulating evidence suggests that codeletion of the tumor suppressor genes Pten and p53 plays a crucial role in development castration-resistant prostate cancer vivo. However, molecular mechanism underlying Pten-/p53-deficiency-driven tumorigenesis remains incompletely understood. Building upon insights gained from our studies with Pten-/p53-deficient mouse embryonic fibroblasts (MEFs), we report here hexokinase 2 (HK2) is selectively upregulated by combined loss cells. Mechanistically,...
Mutations in Met have been identified human papillary renal carcinomas. We shown previously that these mutations deregulate the enzymatic activity of and NIH 3T3 cells expressing mutationally activated are transformed vitro tumorigenic vivo . In present investigation, we find mutant induces motility Madin-Darby canine kidney experimental metastasis , Ras-Raf-MEK-ERK signaling pathway, which has implicated cellular metastasis, is constitutively by mutants. also report transgenic mice...
Loss of function in the von Hippel-Lindau (VHL) tumor suppressor gene occurs familial and most sporadic renal cell carcinomas (RCCs). VHL has been linked to regulation cycle cessation (G(0)) control expression various mRNAs such as for vascular endothelial growth factor. RCC cells express Met receptor tyrosine kinase, mediates invasion branching morphogenesis many types response hepatocyte factor/scatter factor (HGF/SF). We examined HGF/SF responsiveness containing endogenous mutated (mut)...
Abstract The role of NF-κB in the expression inflammatory genes and its participation overall process chronic diseases acute tissue injury are well established. We others have demonstrated a critical involvement poly(ADP-ribose) polymerase (PARP)-1 during inflammation, part, through relationship with NF-κB. However, mechanism by which PARP-1 affects activation has been elusive. In this study, we show that inhibition gene knockout, knockdown, or pharmacologic blockade prevented p65 nuclear...
During glutaminolysis, glutamine is catabolized to glutamate and incorporated into citric acid cycle lipogenesis. Serum levels were measured in patients with primary prostate cancer or metastatic castrate-resistant (mCRPCa) establish clinical relevance. The effect of deprivation blockade by metabotropic receptor 1 (GRM1) antagonists was investigated on cells' growth, migration, invasion biologic relevance.Serum normal men (n = 60) 197) mCRPCa 109). GRM1 expression prostatic tissues examined...
Cofilin (CFL) is an F-actin-severing protein required for the cytoskeleton reorganization and filopodia formation, which drives cell migration. CFL binding severing of F-actin controlled by Ser3 phosphorylation, but contributions this step to migration during invasion metastasis cancer cells are unclear. In study, we addressed question in prostate cells, including response TGF-β, a critical regulator expressing wild-type CFL, TGF-β treatment increased LIMK-2 activity cofilin decreasing...
Prostate cancer (PCa) is the most prevalent cancer, a significant contributor to morbidity and leading cause of cancer-related death in men Western industrialized countries. In contrast genetic changes that vary among individual cases, somatic epigenetic alterations are early highly consistent events. Epigenetics encompasses several different phenomena, such as DNA methylation, histone modifications, RNA interference, genomic imprinting. Epigenetic processes regulate gene expression can...
Abstract The mechanisms underlying the invasive properties of glio‐mas, major form intrinsic brain tumours in humans, are poorly understood. We have reported that CD44 plays an important role this behaviour vitro . In present work, we investigated its ligand, hyaluronic acid (HA), invasion 8 human glioma cell lines. found HA mediates detachment via interaction with high affinity receptor, CD44H. Using μm porosity polycarbonate filter transwells, demonstrate strongly stimulates migration all...
Lethal factor is a protease, one component of Bacillus anthracis exotoxin, which cleaves many the mitogen-activated protein kinase kinases (MEKs). Given importance MEK signaling in tumorigenesis, we assessed effects anthrax lethal toxin (LeTx) on tumor cells. LeTx was very effective inhibiting activation V12 H-ras-transformed NIH 3T3 In vitro , treatment transformed cells with caused them to revert nontransformed morphology, and inhibited their abilities form colonies soft agar invade...
// Prashant K. Singh 1 , Leah Preus 2 Qiang Hu 3 Li Yan Mark D. Long Carl Morrison 4 Mary Nesline Candace S. Johnson Shahriar Koochekpour 5 Manish Kohli 6 Song Liu Donald L. Trump 7 Lara E Sucheston-Campbell 2,* and Moray J. Campbell 1,* Department of Pharmacology & Therapeutics, Roswell Park Cancer Institute, Buffalo, NY Prevention Control, Biostatistics Bioinformatics, Pathology, Genetics, Medical Oncology, Mayo Clinic, Rochester, MN Medicine, * The authors share position as senior...
Reduction or depletion of mitochondrial DNA (mtDNA) has been associated with cancer progression. Although imbalanced mtDNA content is known to occur in prostate cancer, differences between African American (AA) and Caucasian (CA) men are not defined. We provide the first evidence that tumors AA possess reduced level compared CA men. The median tumor was also normal tissues men, suggesting a possible predisposition benign prostatic hyperplasia (BPH) tissue from Tumor BPH patients ≥ 60 years...
Abstract Polycyclic aromatic hydrocarbons (PAHs) are the products of incomplete combustion organic materials, which present in cigarette smoke, deep‐fried food, and natural crude oil. Since PAH‐metabolites form DNA adducts cause oxidative damage, we asked if these environmental carcinogens could affect transforming potential human Polyomavirus JC oncoprotein, T‐antigen (JCV T‐antigen). We extracted DMSO soluble PAHs from Deepwater Horizon oil spill Gulf Mexico (oil‐PAHs), detected several...
High-risk populations exhibit early transformation of localized prostate cancer (CaP) disease to metastasis which results in the mortality such patients. The paucity knowledge about molecular mechanism involved acquiring metastatic behavior by primary tumor cells and non-availability reliable phenotype-discriminating biomarkers are stumbling blocks management CaP disease. Here, we determine role translational relevance ROBO1 (an organogenesis-associated gene) human CaP. Employing...
African–American (AA) patients with prostate cancer (PCa) respond poorly to current therapy compared Caucasian American (CA) PCa patients. Although underlying mechanisms are not defined, mitochondrial dysfunction is a key reason for this disparity. Cell death, cell cycle, and function/stress were analysed by flow cytometry or Seahorse XF24 analyzer. Expression of cellular proteins was determined using immunoblotting real-time PCR analyses. survival/motility evaluated clonogenic, migration,...