A5045672589- Aortic aneurysm repair treatments
- PARP inhibition in cancer therapy
- Aortic Disease and Treatment Approaches
- Asthma and respiratory diseases
- Coronary Interventions and Diagnostics
- Macrophage Migration Inhibitory Factor
- Lipoproteins and Cardiovascular Health
- Ion Channels and Receptors
- Immune cells in cancer
- Cardiac Valve Diseases and Treatments
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- IL-33, ST2, and ILC Pathways
- Infectious Aortic and Vascular Conditions
- Mangiferin and Mango Extracts
- NF-κB Signaling Pathways
- Connective tissue disorders research
- Antimicrobial Resistance in Staphylococcus
- Magnesium in Health and Disease
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Muscle Physiology and Disorders
- Pulmonary Hypertension Research and Treatments
University of Missouri
2017-2023
Nationwide Children's Hospital
2011-2020
Harry S. Truman Memorial Veterans' Hospital
2019
The Ohio State University
2009-2017
Geisinger Medical Center
2012
Louisiana State University Health Sciences Center New Orleans
2006-2010
Mie University
2010
Centre Hospitalier Régional et Universitaire de Nancy
2010
Institute of Pharmacology
2009
Louisiana State University
2007
Journal Article Bayesian lasso regression Get access Chris Hans Department of Statistics, The Ohio State University, Columbus, 43210, U.S.A.hans@stat.osu.edu Search for other works by this author on: Oxford Academic Google Scholar Biometrika, Volume 96, Issue 4, December 2009, Pages 835–845, https://doi.org/10.1093/biomet/asp047 Published: 24 September 2009 history Received: 01 July 2008 Revision received: March
Macrophages are essential for skeletal muscle homeostasis, but how their dysregulation contributes to the development of fibrosis in disease remains unclear. Here, we used single-cell transcriptomics determine molecular attributes dystrophic and healthy macrophages. We identified six clusters unexpectedly found that none corresponded traditional definitions M1 or M2 Rather, predominant macrophage signature was characterized by high expression fibrotic factors, galectin-3 (gal-3) osteopontin...
Several clinical studies have shown the benefits of renin-angiotensin system (RAS) blockade in development diabetes, and a local RAS has been identified pancreatic islets. Angiotensin I-converting enzyme (ACE)2, new component RAS, pancreas, but its role β-cell function remains unknown. Using 8- 16-week-old obese db/db mice, we examined ability ACE2 to alter thereby modulate hyperglycemia.Both nondiabetic lean control (db/m) mice were infected with an adenovirus expressing human...
Abstract The role of NF-κB in the expression inflammatory genes and its participation overall process chronic diseases acute tissue injury are well established. We others have demonstrated a critical involvement poly(ADP-ribose) polymerase (PARP)-1 during inflammation, part, through relationship with NF-κB. However, mechanism by which PARP-1 affects activation has been elusive. In this study, we show that inhibition gene knockout, knockdown, or pharmacologic blockade prevented p65 nuclear...
Aortic valve calcification is the most common form of valvular heart disease, but mechanisms calcific aortic disease (CAVD) are unknown. NOTCH1 mutations associated with malformations and adult-onset in families inherited disease. The Notch signaling pathway critical for multiple cell differentiation processes, its role development CAVD not well understood. aim this study was to investigate molecular changes that occur inhibition valve. members expressed adult cusps, examination diseased...
MicroRNA miR145 has been implicated in vascular smooth muscle cell differentiation, but its mechanisms of action and downstream targets have not fully defined.
Men with castration-resistant prostate cancer (CRPC) face poor prognosis and increased risk of treatment-incurred adverse effects resulting in one the highest mortalities among patient population globally. Immune cells act as double-edged sword depending on tumor microenvironment, which leads to infiltration pro-tumor (M2) macrophages. Development new immunomodulatory therapeutic agents capable targeting hence orchestrating transformation M2 macrophages anti-tumor M1, would substantially...
Poly(ADP-ribose) polymerase (PARP) was suggested to play a role in endothelial dysfunction that is associated with number of cardiovascular diseases. We hypothesized PARP may an important atherogenesis and its inhibition attenuate atherosclerotic plaque development experimental model atherosclerosis.Using mouse (apolipoprotein E [ApoE](-/-)) high-fat diet-induced atherosclerosis, we demonstrate association between cell death oxidative stress-associated DNA damage activation within plaques....
Activation of inflammatory pathways plays a critical role in the development abdominal aortic aneurysms (AAA). Notch1 signaling is significant regulator response; however, its AAA unknown.In an angiotensin II-induced mouse model AAA, activation was observed aneurysmal tissue Apoe(-/-) mice, and similar humans undergoing repair. haploinsufficiency significantly reduced incidence mice response to II. Reconstitution bone marrow-derived cells from Notch1(+/-);Apoe(-/-) (donor) lethally...
Background The progression of abdominal aortic aneurysm ( AAA ) involves a sustained influx proinflammatory macrophages, which exacerbate tissue injury by releasing cytokines, chemokines, and matrix metalloproteinases. Previously, we showed that Notch deficiency reduces the development in angiotensin II –induced mouse model preventing infiltration macrophages. Here, examined whether inhibition this prevents small these effects are associated with altered macrophage differentiation. Methods...
Abstract We recently used a murine model of allergic airway inflammation to show that poly(ADP-ribose) polymerase-1 (PARP-1) plays an important role in the pathogenesis asthma-related lung inflammation. In this study, we PARP-1 inhibition, by novel inhibitor (TIQ-A) or gene deletion, prevented eosinophilic infiltration into airways OVA-challenged mice. Such impairment eosinophil recruitment appeared take place after IgE production. OVA challenge wild-type mice resulted significant increase...
Abstract The role of inducible NO synthase (iNOS) in allergic airway inflammation remains elusive. We tested the hypothesis that iNOS plays different roles during acute versus chronic inflammation. Acute and mouse models OVA-induced were used to conduct study. showed deletion was associated with a reduction eosinophilia, mucus hypersecretion, IL-5 IL-13 production upon protocol. Such protection completely abolished Interestingly, pulmonary fibrosis observed wild-type mice under protocol...
Aims Infiltration of macrophages and apoptosis vascular smooth muscle cells (VSMCs) promote the development abdominal aortic aneurysm (AAA). Previously, we demonstrated that global Notch1 deficiency prevents formation AAA in a mouse model. Herein, sought to explore cell-specific roles development. Methods results Cell-specific haploinsufficient mice, generated on Apoe-/- background using Cre-lox technology, were infused with angiotensin II (1000 ng/min/kg) for 28 days. haploinsufficiency...
Objective— Abdominal aortic aneurysm is caused by the accumulation of inflammatory cells in wall. Our recent studies demonstrated that inhibition Notch signaling attenuates abdominal formation shifting macrophage balance towards anti-inflammatory (M2) phenotype. Using IL12p40 −/− (interleukin 12 p40) mice, we investigated effects M2-predominant macrophages on development aneurysm. Approach and Results— Male (8–10 week-old) wild-type mice (n=15) C57BL/6 background were infused with Ang II...
Angiotensin-converting enzyme type 2 (ACE2) has been shown to be an important member of the renin angiotensin system. Previously, we observed that central ACE2 reduces development hypertension following chronic II (Ang-II) infusion in syn-hACE2 transgenic (SA) mice, which human transgene is selectively targeted neurons. To study physiological consequences over-expression on cardiac function and hypertrophy, SA non-transgenic (NT) mice were infused with Ang-II (600 ng/kg/min, sc) for 14 days,...
The aim of this study was to take a combination animal and cell culture approaches examine the individual responses vascular cells varying inflammatory factors in order gain insights on mechanism(s) by which poly(ADP-ribose) polymerase (PARP) inhibition promotes plaque stability. Apolipoprotein (ApoE−/−) mice fed high-fat diet were used as model atherosclerosis. Primary endothelial cells, smooth muscle (SMCs), ex-vivo generated foam (FCs) our vitro studies. PARP significantly decreased...
We recently showed that poly(ADP-ribose) polymerase (PARP) is activated within atherosclerotic plaques in an animal model of atherosclerosis. Pharmacological inhibition PARP or reduced expression heterozygous animals interferes with atherogenesis and may promote factors plaque stability, possibly reflecting changes inflammatory cellular consistent stability. The current study addresses the hypothesis pharmacological promotes regression. Using a high-fat diet-induced atherosclerosis...
Abstract Abdominal aortic aneurysm (AAA) is characterized by transmural infiltration of myeloid cells at the vascular injury site. Previously, we reported preventive effects Notch deficiency on development AAA reduction infiltrating cells. In this study, examined if inhibition attenuates progression pre-established and potential implications. Pharmacological inhibitor (N-[N-(3,5-difluorophenacetyl)-L-alanyl]-(S)-phenylglycine t-butyl ester; DAPT) was administered subcutaneously three times a...
Naïve macrophages (Mφ) polarize in response to various environmental cues a spectrum of cells that have distinct biological functions. The extreme ends the are classified as M1 and M2 macrophages. Previously, we demonstrated Notch1 deficiency promotes Tgf-β2 dependent M2-polarization mouse model abdominal aortic aneurysm. present studies aimed characterize unique set genes regulated by signaling macrophage polarization. Bone marrow derived isolated from WT or