A5060963937- Urinary Tract Infections Management
- Urinary Bladder and Prostate Research
- Pediatric Urology and Nephrology Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Immune Response and Inflammation
- Electrospun Nanofibers in Biomedical Applications
- Parasites and Host Interactions
- Gut microbiota and health
- Aortic aneurysm repair treatments
- Macrophage Migration Inhibitory Factor
- Infectious Aortic and Vascular Conditions
- Immune Cell Function and Interaction
- Escherichia coli research studies
- Tissue Engineering and Regenerative Medicine
- Research on Leishmaniasis Studies
- Ion Channels and Receptors
- Inflammatory Bowel Disease
- Nitric Oxide and Endothelin Effects
- Inflammation biomarkers and pathways
- Bacterial biofilms and quorum sensing
- Diabetes Treatment and Management
- Galectins and Cancer Biology
- Cardiac and Coronary Surgery Techniques
- Bladder and Urothelial Cancer Treatments
Nationwide Children's Hospital
2016-2025
The Ohio State University
2023-2024
Tecnológico Nacional de México
2020
Universidad Nacional Autónoma de México
2010-2014
Autonomous University of Tlaxcala
2011
Classically (M1) and alternatively activated (M2) macrophages exhibit distinct phenotypes functions. It has been difficult to dissect macrophage in vivo, where a spectrum of exists, also vitro, low or non-selective M2 marker protein expression is observed. To provide foundation for the complexity vivo phenotypes, we performed comprehensive analysis transcriptional signature murine M0, M1 identified genes common exclusive either subset. We validated by real-time PCR an M1-exclusive pattern...
Cystic fibrosis (CF), one of the most common human genetic diseases worldwide, is caused by a defect in CF transmembrane conductance regulator (CFTR). Patients with are highly susceptible to infections opportunistic pathogens (including Burkholderia cenocepacia), which induce excessive lung inflammation and lead eventual loss pulmonary function. Abundant neutrophil recruitment into key characteristic bacterial patients. In response infection, inflammatory neutrophils release reactive oxygen...
With the emergence of antibiotic-resistant bacteria, innovative approaches are needed for treatment urinary tract infections. Boosting antimicrobial peptide expression may provide an alternative to antibiotics. Here, we developed reporter cell lines and performed a high-throughput screen clinically used drugs identify compounds that boost ribonuclease 4 7 (RNase 7), peptides have activity against uropathogens. This identified histone deacetylase (HDAC) inhibitors as effective RNase inducers....
(1) Background: Obesity and overweight are defined as an abnormal or excessive accumulation of fat that can be harmful to health. These conditions lead a lifetime diseases, including cancer, diabetes, heart disease. The diagnosis obesity in children adolescents depends on the international reference used. (2) Objectives: In this study aimed determine level concordance prevalence underweight, normal weight, overweight, were estimated with three references Mexican between 2 18 years age. (3)...
Obesity is a significant public health concern that associated with numerous risks. Infections are major complication of obesity, but the mechanisms responsible for increased infection risk poorly defined. Here, we use diet induced obesity mouse model and investigate how impacts urinary tract (UTI) susceptibility bladder immune defenses. Our results show high-fat fed female male mice exhibit to uropathogenic E. coli (UPEC) following experimental UTI. Transcriptomic analysis urothelial cells...
The aryl hydrocarbon receptor (AhR) is part of a signaling system that mainly triggered by xenobiotic agents. Increasing evidence suggests AhR may regulate immunity to infections. To determine the role in outcome toxoplasmosis, we used AhR-/- and wild-type (WT) mice. Following an intraperitoneal infection with Toxoplasma gondii ( T. ), mice succumbed significantly faster than WT displayed greater liver damage as well higher serum levels tumor necrosis factor (TNF)-<mml:math...
Abstract Helminths cause chronic infections and affect the immune response to unrelated inflammatory diseases. Although helminths have been used therapeutically ameliorate conditions, their anti-inflammatory properties are poorly understood. Alternatively activated macrophages (AAMϕs) suggested as effector cells during helminth infections. Here, we define origin of AAMϕs infection with Taenia crassiceps , disease-modulating activity on Experimental Autoimmune Encephalomyelitis (EAE). Our...
Macrophage migration inhibitory factor (MIF) mediates immunity against Toxoplasma gondii infection by inducing inflammatory cytokines required to control the parasite replication. However, role of this mediator in cell-mediated immune response is still poorly understood. Here, we used T. -infected WT and Mif −/− mice analyze MIF maturation CD11b + CD8 α dendritic cells (DCs). We found that promotes but not DCs, IL-12p70 production CD86 expression. Infected showed significantly lower numbers...
Stenosis is a critical problem in the long-term efficacy of tissue-engineered vascular grafts (TEVGs). We previously showed that host monocyte infiltration and activation within graft drives stenosis TGF-β receptor 1 (TGF-βR1) inhibition can prevent it, but latter effect was attributed primarily to mesenchymal cell expansion. In this study, we assessed effects TGF-βR1 on monocytes. Biodegradable TEVGs were implanted as inferior vena cava interposition conduits 2 groups C57BL/6 mice (n =...
Abstract Abdominal aortic aneurysm (AAA) is characterized by transmural infiltration of myeloid cells at the vascular injury site. Previously, we reported preventive effects Notch deficiency on development AAA reduction infiltrating cells. In this study, examined if inhibition attenuates progression pre-established and potential implications. Pharmacological inhibitor (N-[N-(3,5-difluorophenacetyl)-L-alanyl]-(S)-phenylglycine t-butyl ester; DAPT) was administered subcutaneously three times a...
During infection, phagocytic cells pursue homeostasis in the host via multiple mechanisms that control microbial invasion. Neutrophils respond to infection by exerting a variety of cellular processes, including chemotaxis, activation, phagocytosis, degranulation and generation reactive oxygen species (ROS). Calcium (Ca2+) signaling activation specific Ca2+ channels are required for most antimicrobial effector functions neutrophils. The transient receptor potential melastatin-2 (TRPM2) cation...
Aim: To characterize early events in neotissue formation during the first 2 weeks after vascular scaffold implantation. Materials & methods: Biodegradable polymeric scaffolds were implanted as abdominal inferior vena cava interposition grafts wild-type mice. Results: All explanted at day 1 contained a platelet-rich mural thrombus. Within few days, majority of cell infiltration appeared to be from myeloid cells peritoneal surface with modest along lumen. Host reaction graft was distinct...
Neutrophils, which constitute the most abundant leukocytes in human blood, emerge as crucial players induction of endothelial cell death and modulation responses under both physiological pathological conditions. The hallmark preeclampsia is dysfunction induced by systemic inflammation, neutrophils, particularly through formation neutrophil extracellular traps (NETs), play a pivotal role development perpetuation hypertensive state. Considering potential numerous pharmaceutical agents to...
Introduction Therapeutic antibodies have become a major strategy to treat oncologic diseases. For chronic lymphocytic leukemia, against CD20 are used target and elicit cytotoxic responses malignant B cells. However, efficacy is often compromised due suppressive microenvironment that interferes with cellular immune responses. To overcome this suppression, agonists of pattern recognition receptors been studied which promote direct cytotoxicity or anti-tumoral NOD2 an intracellular receptor...
Objective— Despite successful translation of bioresorbable vascular grafts for the repair congenital heart disease, stenosis remains primary cause graft failure. In this study, we investigated efficacy long-term treatment with antiplatelet drugs, aspirin and cilostazol, in preventing evaluated effect these drugs on acute phase inflammation tissue remodeling. Approach Results— C57BL/6 mice were fed a drug-mixed diet aspirin, or normal chow during course follow-up. Bioresorbable grafts,...
Significance Statement More than a half of urinary tract infections in infants spread from the bladder to kidneys and cause acute pyelonephritis, which may lead renal scarring, hypertension, subsequent loss kidney function. However, cellular mechanisms underlying permanent damage after an pyelonephritis episode are unknown. In this study, authors used preclinical rodent model pyelonephritis–mediated scarring evaluate contribution immune phagocytes resolution condition. The data demonstrated...
Mounting evidence suggests that antimicrobial peptides and proteins (AMPs) belonging to the RNase A superfamily have a critical role in defending bladder kidney from bacterial infection. 6 has been identified as potent, leukocyte-derived AMP, but its impact on urinary tract infection (UTI) vivo not demonstrated. To test functional of human 6, we generated <i>RNASE6</i> transgenic mice studied their susceptibility experimental UTI. In addition, bone marrow-derived macrophages...
ABSTRACT We previously developed a tissue‐engineered vascular graft (TEVG) made by seeding autologous cells onto biodegradable tubular scaffold, in an attempt to create living with growth potential for use children undergoing congenital heart surgery. Results of our clinical trial showed that the TEVG possesses capacity but its widespread is not yet advisable due high incidence stenosis. In animal models, stenosis caused increased monocytic cell recruitment and classic (“M1”) activation....
The C-type lectin receptor mMGL is expressed exclusively by myeloid antigen presenting cells (APC) such as dendritic (DC) and macrophages (Mφ), it mediates binding to glycoproteins carrying terminal galactose αor β-N-acetylgalactosamine (Gal/GalNAc) residues.Trypanosoma cruzi (T.cruzi) expresses large amounts of mucin (TcMUC)-like glycoproteins.Here, we show lectin-blot that moieties are also on the surface T. cruzi.Male knockout (-/-) wild-type (WT) C57BL/6 mice were infected...
The ribonuclease (RNase) A superfamily encodes cationic antimicrobial proteins with potent microbicidal activity toward uropathogenic bacteria. Ribonuclease 6 (RNase6) is an evolutionarily conserved, leukocyte-derived peptide Escherichia coli (UPEC), the most common cause of bacterial urinary tract infections (UTIs). In this study, we generated Rnase6-deficient mice to investigate hypothesis that endogenous RNase limits host susceptibility UTI.
Abstract Aim Sodium‐glucose co‐transporter‐2 (SGLT2) inhibitors have revolutionized clinical medicine, but their association with urinary tract infection (UTI) risk remains debated. This study investigates the influence of dapagliflozin on UTI outcomes, focusing kidney injury. Materials and Methods Female non‐diabetic C57BL/6J C3H/HeOuJ mice, along diabetic db / were orally administered (1 mg/kg or 10 mg/kg) for 7 days before transurethral uropathogenic Escherichia coli (UPEC) infection....
The precise role of neutrophil-derived reactive oxygen species (ROS) in combating bacterial uropathogens during urinary tract infections (UTI) remains largely unexplored. In this study, we elucidate the antimicrobial significance NADPH oxidase 2 (NOX2)-derived ROS, as opposed to mitochondrial facilitating neutrophil-mediated eradication uropathogenic Escherichia coli (UPEC), primary causative agent UTI. Furthermore, NOX2-derived ROS regulate NF-κB-mediated inflammatory responses neutrophils...
Nontypeable Haemophilus influenzae (NTHi) causes multiple diseases of the human airway and is a predominant bacterial pathogen acute otitis media in which treatment fails. NTHi utilizes system phase variable epigenetic regulation, termed phasevarion, to facilitate adaptation survival within sites host. The phasevarion influences numerous disease-relevant phenotypes such as biofilm formation, antibiotic resistance opsonization. We have previously identified an advantageous selection for...