A5063171142- Muscle Physiology and Disorders
- Neurogenetic and Muscular Disorders Research
- Cardiomyopathy and Myosin Studies
- Immune Cell Function and Interaction
- Ion channel regulation and function
- Mesenchymal stem cell research
- Exercise and Physiological Responses
- Muscle activation and electromyography studies
- IL-33, ST2, and ILC Pathways
- Adipose Tissue and Metabolism
- Species Distribution and Climate Change
- Knee injuries and reconstruction techniques
- Genetic Neurodegenerative Diseases
- Pleistocene-Era Hominins and Archaeology
- Adipokines, Inflammation, and Metabolic Diseases
- Evolution and Paleontology Studies
John Brown University
2020-2025
Brown University
2020-2024
Providence College
2024
University of California, Irvine
2023
Macrophages are essential for skeletal muscle homeostasis, but how their dysregulation contributes to the development of fibrosis in disease remains unclear. Here, we used single-cell transcriptomics determine molecular attributes dystrophic and healthy macrophages. We identified six clusters unexpectedly found that none corresponded traditional definitions M1 or M2 Rather, predominant macrophage signature was characterized by high expression fibrotic factors, galectin-3 (gal-3) osteopontin...
Myofiber size regulation is critical in health, disease, and aging. MuSK (muscle-specific kinase) a BMP (bone morphogenetic protein) co-receptor that promotes shapes signaling. expressed at all neuromuscular junctions also present extrasynaptically the mouse soleus, whose predominantly oxidative fiber composition akin to of human muscle. To investigate role MuSK-BMP pathway vivo, we generated mice lacking BMP-binding Ig3 domain. These ∆Ig3-MuSK are viable fertile with innervation levels...
The liver harbors two main innate lymphoid cell (ILC) populations: conventional NK (cNK) cells and tissue-resident (trNK) cells. Using the MCMV model of infection, we find that, in contrast to cNK cells, trNK initially undergo a contraction phase followed by recovery homeostatic levels. is independent because similar phenotype observed following poly(I:C)/CpG or α-GalCer injection. rapid due apoptosis, whereas occurs via proliferation situ. Interestingly, apoptosis not mediated fratricide...
The neuromuscular junction (NMJ) is the linchpin of nerve-evoked muscle contraction. Broadly, function NMJ to transduce nerve action potentials into fiber (MFAPs). Efficient transmission requires both cholinergic signaling, responsible for generation endplate (EPPs), and excitation, amplification EPP by postsynaptic voltage-gated sodium channels (Nav1.4) generate MFAP. In contrast component, signaling pathways that organize Nav1.4 mediate excitability are poorly characterized....
SUMMARY A central question in adult stem cell biology is elucidating the signaling pathways regulating their dynamics and function diverse physiological age-related contexts. Muscle cells adults (Satellite Cells; SCs) are generally quiescent but can activate contribute to muscle repair growth. Here we tested role of MuSK-BMP pathway SC quiescence by deletion BMP-binding MuSK Ig3 domain (‘ΔIg3-MuSK’). At 3 months age myonuclei numbers myofiber size were comparable WT. However, at 5 density...
The monocytic/macrophage system is essential for skeletal muscle homeostasis, but its dysregulation contributes to the pathogenesis of degenerative disorders. Despite our increasing knowledge role macrophages in disease, it still remains unclear how contribute fibrosis. Here, we used single-cell transcriptomics determine molecular attributes dystrophic and healthy macrophages. We identified six novel clusters. Unexpectedly, none corresponded traditional definitions M1 or M2 macrophage...
The neuromuscular junction (NMJ) is the linchpin of nerve-evoked muscle contraction. Broadly considered, function NMJ to transduce a nerve action potential into fiber (MFAP). Efficient information transfer requires both cholinergic signaling, responsible for generation endplate potentials (EPPs), and excitation, activation postsynaptic voltage-gated sodium channels (Nav1.4) trigger MFAPs. In contrast apparatus, signaling pathways that organize Nav1.4 excitability are poorly characterized....
A central question in adult stem cell biology is elucidating the signaling pathways regulating their dynamics and function diverse physiological age-related contexts. Muscle cells adults (Satellite Cells; SCs) are generally quiescent but can activate contribute to muscle repair growth. Here we tested role of MuSK-BMP pathway SC quiescence by deletion BMP-binding MuSK Ig3 domain (‘ΔIg3-MuSK’). At 3 months age myonuclei numbers myofiber size were comparable WT. However, at 5 density was...
A central question in adult stem cell biology is elucidating the signaling pathways regulating their dynamics and function diverse physiological age-related contexts. Muscle cells adults (Satellite Cells; SCs) are generally quiescent but can activate contribute to muscle repair growth. Here we tested role of MuSK-BMP pathway SC quiescence by deletion BMP-binding MuSK Ig3 domain (‘ΔIg3-MuSK’). At 3 months age myonuclei numbers myofiber size were comparable WT. However, at 5 density was...
Abstract Myofiber size regulation is critical in health, disease, and aging. MuSK (muscle-specific kinase) a BMP (bone morphogenetic protein) co-receptor that promotes shapes signaling. expressed at all neuromuscular junctions also present extrasynaptically the slow soleus muscle. To investigate role of MuSK-BMP pathway vivo we generated mice lacking BMP-binding Ig3 domain. These ∆Ig3-MuSKmice are viable fertile with innervation levels comparable to wild type. In 3-month-old myofibers...
Abstract The liver harbors two main innate lymphoid cell (ILC) populations, conventional NK (cNK) cells and tissue-resident (trNK) also called ILC1s. While the antiviral functions of cNK have been well established, trNK are only beginning to be uncovered. Using MCMV model infection, we found that in contrast cells, population initially undergoes a contraction phase prior recovery homeostatic levels. rapid is due apoptosis, while occurs via proliferation situ. Liver apoptosis not mediated by...
ABSTRACT Myofiber size regulation is critical in health, disease, and aging. MuSK (muscle-specific kinase) a BMP (bone morphogenetic protein) co-receptor that promotes shapes signaling. expressed at all neuromuscular junctions also present extrasynaptically the slow soleus muscle. To investigate role of MuSK-BMP pathway vivo we generated mice lacking BMP-binding Ig3 domain. These ΔIg3-MuSK are viable fertile with innervation levels comparable to wild type. In 3-month-old myofibers smaller...