Staphylococcus infection-associated GN (SAGN) is a well recognized disease entity, particularly because of the frequent IgA-dominant glomerular immunoglobulin staining on kidney biopsy. Biopsy features can resemble two other entities - primary IgA nephropathy and Henoch-Schönlein purpura nephritis posing diagnostic pitfall. This clinically relevant crucial difference in therapeutic approach. The diagnosis SAGN further complicated by variability degree (and C3) staining, extent electron dense...

<i>Background/Aims:</i> We previously reported that patients with chronic kidney disease (CKD) receiving warfarin therapy and whose international normalized ratio increases to >3.0 may develop acute injury (AKI) as a result of glomerular hemorrhage formation obstructive red blood cell (RBC) casts. named this condition warfarin-related nephropathy (WRN). also excessive anticoagulation brodifacoum (superwarfarin) induces AKI in 5/6 nephrectomy (5/6NE) rats. Limitations the model...

Anticoagulant-related nephropathy (ARN) was initially described in patients on warfarin (as warfarin-related nephropathy) and recently those using dabigatran. Herein, we report clinical history kidney biopsy findings a patient apixaban (Eliquis). Initiation of treatment with resulted aggravation preexisting mild acute injury (AKI). A few days after therapy, the became oligoanuric, showed severe tubular necrosis numerous occlusive red blood cell casts. Only one out 68 glomeruli open capillary...

Background Chronic kidney disease (CKD) is a leading cause of death, its progression driven by glomerular podocyte injury and loss, manifesting as proteinuria. Proteinuria includes loss coagulation zymogens, cofactors, inhibitors resulting in hypercoagulable state characterized enhanced thrombin generation. Both CKD proteinuria significantly increase the risk thromboembolic disease. Meanwhile, anticoagulant medications (which antagonize thereby prevent thromboembolism) have been shown to...

The purpose of the study was to test hypothesis that neutrophils can injure cultured skeletal myotubes. Human myotubes were grown and then with human blood neutrophils. Myotube injury quantitatively qualitatively determined using a cytotoxicity ( 51 Cr) assay electron microscopy, respectively. For Cr assay, neutrophils, under non-in vitro-stimulated N-formylmethionyl-leucyl-phenylalanine (FMLP)-stimulated conditions, at effector-to-target cell (E:T) ratios 10, 30, 50 for 6 h. Statistical...

Alveolar type II (ATII) epithelial cells are the primary site of influenza virus replication in distal lung. Development acute respiratory distress syndrome influenza-infected mice correlates with significant alterations ATII cell function. However, impact infection on surfactant lipid metabolism has not been explored. C57BL/6 were inoculated intranasally A/WSN/33 (H1N1) (10,000 plaque-forming units/mouse) or mock-infected diluent. isolated by a standard lung digestion protocol at 2 and 6...

We investigated human calicivirus (HCV)-associated diarrheain children attending day care centers by using stool specimens collected in 1981–1983. used a screening enzyme linked immunosorbent assay (ELISA) derived from reagents prepared against the Sapporostrain ofHCV and confirmed positive results with blocking ELISA an dimmunosorbent electron microscopy. HCV was detected 11 (2.9%) of 375 diarrheal stools none 86 asymptomatic contacts. This incidence ratewas half that noted for rotaviruses...

Cutaneous leishmaniasis (CL) manifests as localized skin lesions, which lead to significant tissue destruction and disfigurement. In the Yucatan Peninsula, Mayan traditional healers use Pentalinon andrieuxii Muell.‐Arg. (Apocynaceae) roots for topical treatment of CL. Here, we studied effect P. root hexane extract (PARE) on parasites host cells in vitro examined its efficacy CL caused by Leishmania mexicana . PARE exhibited potent antiparasitic activity against promastigotes well amastigotes...

Technical parameters of electron microscope staining procedures (type stain, pH and time staining) influence particle integrity for three groups rotaviruses. Simian rotavirus SA11 (group A), Chinese adult diarrhea porcine rotavirus-like agent B), pararotavirus C) were tested. All strains quite stable in uranyl acetate phosphotungstic acid at 4.5 relatively ammonium molybdate. However, with higher values increased yielded a reduction the number particles that broken or degraded to...

Abstract NK dendritic cells (DCs; NKDCs) appear to emerge as a distinct DC subset in humans and rodents, which have the functions of DCs. However, developmental relationship NKDCs (CD11c+NK1.1+) CD11c+NK1.1– DCs has not been addressed. Herein, we show that exist exclusively compartment CD11c+MHC II– steady state express variable levels markers, such IFN-producing killer marker B220, tissue-dependent manner. They can differentiate into NK1.1– DCs, is accompanied by up-regulation MHC Class II...

Abstract The quantity and quality of collagen fibrils in the extracellular matrix (ECM) have a pivotal role dictating biological processes. Several collagen-binding proteins (CBPs) are known to modulate deposition fibril diameter. However, limited studies exist on alterations ultrastructure by CBPs. In this study, we elucidate how receptor, discoidin domain receptor 1 (DDR1) regulates content adventitia DDR1 knock-out (KO) mice. KO mice exhibit increased as observed using Masson’s trichrome....

One thousand and eighty patients, having prolonged bleeding times, frequent epistaxis, menorrhagia or easy bruising other manifestations, excluding those with von Willebrand’s disease, were evaluated for platelet dense granule deficiency. The mean diameter of granules was determined all patients using image analysis. Four hundred ninety-nine had “classic” (delta) storage pool deficiency (δ-SPD). Five eighty-one individuals (53.8%) found to have a normal number granules, but some these the...

Monoclonal gammopathies can cause renal tubular epithelial damage through multiple mechanisms, the most common manifestation being myeloma cast nephropathy. Amyloid light chain amyloidosis rarely affects epithelium directly and usually causes glomerular injury. deposition also be seen within vessel walls in tubulointerstitium. Herein, we describe a unique pattern of κ amyloid involving proximal tubule patient with myeloma, characterized by intracellular globule formation concomitant...

Spironolactone is often used to treat hypertension caused by hyperaldosteronism, and as a result, can form concentrically laminated electron dense spironolactone body inclusions within the adrenal gland. bodies have not been investigated in contemporary cohort or patients treated with more recently approved aldosterone antagonist, eplerenone.Spironolactone were retrospectively for hyperaldosteronism (n=15) from 2012-2013 that underwent subsequent adrenalectomy.Inclusions identified 33% of...

Members of the Rab GTPase family regulate intracellular protein trafficking, but specific function Rab24 remains unknown. Several attributes distinguish this from other members family, including a low intrinsic activity. The functions proteins have been defined through use dominant-negative mutants with amino acid substitutions in conserved N(T)KxD nucleotide binding motif. Surprisingly, when such constructs were expressed cultured cells, they accumulated nuclear inclusions which disrupted...

Humoral immunity contributes significantly to lung graft dysfunction. Recognizing a role of ultrastructural studies in the evaluation and diagnosis chronic humoral allograft rejection kidney, authors sought explore its utility as diagnostic adjunct biopsies. Ultrastructural were conducted on 44 biopsies from 26 transplant recipients. Endothelial cell activation necrosis seen setting acute rejection. Septal vasculopathic changes thickening lamellation basement membrane zone (BMZ) BMZ collagen...

Abstract Context.—Consensus exists among renal pathologists that, in biopsies with immune complex glomerulonephritis, even a single glomerulus open capillary loops may be sufficient for immunofluorescence and/or electron microscopy evaluation because deposition is diffuse phenomenon. However, we have encountered focal absence of complexes glomeruli on either or examination despite presence glomerular loops. Objective.—To evaluate and look any subtle unusual morphologic changes the (and...

Fibrillary glomerulonephritis (FGN) is characterized by the deposition of IgG-positive, randomly arranged, nonbranching, non-Congophilic fibrils in glomeruli. The possibility multiorgan involvement, as amyloidosis, has been raised. authors report first detailed autopsy study on a patient with FGN, thorough examination organs electron microscopy, colloidal gold immunoelectron and immunofluorescence staining. Thin, wavy (extracellular matrix filaments) ranging from 6 to 17 nm were seen all...