Von Willebrand disease (vWD) is a common bleeding disorder with different subtypes. Laboratory diagnosis challenging, involving several expensive and complex assays. The von factor (vWF) collagen binding assay (VWF:CB) has been described to improve the of vWD, but there lack consensus its implementation into guidelines diagnostic algorithms incomplete. A cohort 88 patients inherited vWD 10 investigated for due phenotype were recruited underwent analysis vWF multimers, antigen (VWF:Ag)...

Background Chronic kidney disease (CKD) is a leading cause of death, its progression driven by glomerular podocyte injury and loss, manifesting as proteinuria. Proteinuria includes loss coagulation zymogens, cofactors, inhibitors resulting in hypercoagulable state characterized enhanced thrombin generation. Both CKD proteinuria significantly increase the risk thromboembolic disease. Meanwhile, anticoagulant medications (which antagonize thereby prevent thromboembolism) have been shown to...

For patients with hemophilia A and high-titer inhibitors treated bypassing agents there are no reliable methods to assess treatment effect. We investigated the utility of global hemostatic in assessing (rFVIIa or activated prothrombin complex [aPCC]). All followed at Coagulation Unit Pediatric Karolinska University Hospital aged 6 years above were eligible for this noninterventional study. Baseline plasma samples spiked increasing concentrations (aPCC 50 U/kg, 100 150 rFVIIa 90 μg/kg 270...

Abstract Introduction Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced pre‐eclampsia (P‐EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties women with investigate their association maternal foetal outcomes. Methods Forty‐six pregnant diagnosed were included the study, blood sampling done on morning following admission hospital, as well after delivery (mean duration 4.8 days). Two global...

This study was aimed to assess hemostatic disturbances in female patients with established rheumatoid arthritis (RA) relation menopausal status and disease activity.Ninety women were included the study, 42 48 age-matched healthy controls. There no differences between investigated groups regarding presence of traditional cardiovascular risk factors. Two global assays employed, namely endogenous thrombin potential (ETP) overall hemostasis (OHP). The parameters ETP assay (ETP, C-max, t-lag,...

Thrombosis is a major global disease burden with almost 60% of cases related to underlying heredity and most still idiopathic. Synonymous single nucleotide polymorphisms (sSNPs) are considered silent phenotypically neutral. Our previous study revealed novel synonymous FII c.1824C>T variant as potential risk factor for pregnancy loss, but it has not yet been associated thrombotic diseases.To determine the frequency we have sequenced patients' DNA. Prothrombin RNA expression was measured by...

Abstract Circulating microparticles (MPs) are procoagulant due to the surface containing phosphatidylserine (PS), which facilitates coagulation. We investigated if MPs improve hemostasis in HA plasma models. isolated from pooled normal human were added severe, moderate and mild models (0%, 2.5%, 20% FVIII). The MPs’ effect on was evaluated by calibrated automated thrombogram (CAT) overall potential (OHP) assays, while fibrin structure imaged standard confocal, stimulated emission depletion...

This study sought to identify different subpopulations of extracellular vesicles (EVs) in plasma from female patients with established rheumatoid arthritis (RA) relation the activation coagulation and fibrin formation these patients. Forty women were included study, 20 age-matched healthy controls. The mean disease duration was 13.0 (5.0–25.0) years, medium high activity despite ongoing treatment low-dose prednisolone methotrexate. There no differences between investigated groups regarding...

Recurrent fetal loss (RFL) is common health problem affecting up to 5% of women reproductive age. It has been shown that plasma thrombin-activatable fibrinolysis inhibitor (TAFI) concentrations increase during pregnancy and return baseline levels soon after delivery. The +1040C/T single nucleotide polymorphism in coding region TAFI gene associated with blood levels. aim our study was investigate the relationship between idiopathic RFL. Study carried out a group 120 (61 controls 59 RFL)....

Abstract Development of inhibitors to factor VIII (FVIII) occurs in approximately 30% severe hemophilia A (HA) patients. These patients are treated with bypassing agents (activated prothrombin complex concentrate [aPCC] and recombinant activated FVII-rFVIIa). Recently, a bispecific FIX/FIXa- FX/FXa-directed antibody (emicizumab) has been approved for the treatment HA inhibitors. However, data from clinical studies imply that coadministration emicizumab agents, especially aPCC, could have...

Summary Background: Plasminogen activator inhibitor 1 (PAI-1) is an of fibrinolysis. The PAI-1 4G/5G polymorphism associated with elevated plasma levels PAI-1. Over- expression and impaired fibrinolysis in homozygous carriers the 4G/4G PAI may lead to abnor- mal placental formation increased risk fetal loss (FL). aim our study was determine frequency this patients FL a Serbian population. Methods: carried out group 203 women (91 controls 112 FL). presence detected by PCR-RFLP analysis....

Deep vein thrombosis (DVT) is a multifactorial disease that occurs with frequency of 1/1000 per year. The FV Leiden, FII G20210A and MTHFR C677T mutations represent genetic factors for the occurrence thrombosis. goal this study was to determine these in patients DVT upper lower limbs. encompassed 119 divided two groups. group limbs included 77 patients, while 42 patients. presence detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. In limbs,...

Prothrombin (FII) A19911G and C20221T gene variants are associated with increased prothrombin levels potentially represent thrombotic risk factors.To determine the frequency of FII in patients disorders women who have experienced pregnancy loss (PL).We determined these 133 deep venous thrombosis (DVT), 80 isolated pulmonary embolism (PE), 101 idiopathic PL, 180 control individuals.The variant was more prevalent DVT PL compared controls; however, differences were not statistically...

Objective. To assess thrombin generation, fibrin formation, and structure together with the fibrinolytic status in patients systemic sclerosis (SSc) relation to occurrence of digital ulcers (DUs) during course disease. Methods. We studied variables endothelial dysfunction, overall hemostatic potential, clot turbidity plasma from 58 SSc (39 DU history 19 DU-naïve) 46 matched healthy controls (HCs). Fibrin was visualized using scanning electron microscopy (SEM). Finally, 39 a DUs were followed...

Introduction: Plasminogen activator inhibitor 1 (PAI-1) has a major role in inhibition of firinolysis and normal haemostasis. The presence the PAI-1 4G/4G genotype leads to increased expression PAI-1. High blood level is associated with many diseases such as thrombosis, cerebral insult, myocardial infarction, pregnancy loss, preeclampsia, insulin resistance, type 2 diabetes, breast cancer asthma. In this study, prevalence 4G/5G gene variant was determined healthy subjects from Serbian...

Abstract Introduction Prothrombin Belgrade mutation is the result of c.1787G>A substitution in prothrombin gene. It located antithrombin and sodium binding site leads to impaired inactivation thrombin by antithrombin, resulting resistance thrombotic disorders. However, it negatively affects may have hypocoagulant effects. Considering that mechanism still not fully elucidated important for affinity towards fibrinogen, our aim was determine whether this fibrin clot formation lysis. Methods...

Abstract Introduction For acute venous thromboembolism (VTE), a biomarker with higher specificity than D-dimer would be of great clinical use. Thrombin generation and overall hemostatic potential (OHP) reflect the balance by globally assessing multiple coagulation factors inhibitors. These tests discriminate between healthy controls patients prothrombotic tendency but have yet to established as biomarkers VTE. Objective This study compares endogenous thrombin (ETP) OHP fibrin monomers (FM)...

Thrombophilia is a multifactorial disorder that arises from the interaction of acquired and genetic risk factors. Despite significant efforts made to understand etiology this disease, there are still certain number patients suffering idiopathic thrombophilia. The aim study was screen 3' end prothrombin (FII) gene, which susceptible gain-of-function mutations due its non canonical architecture, in with thrombophilia determine eventual role pathogenesis This carried out 100 healthy controls....

Recurrent pregnancy loss (RPL) is a health problem affecting up to 5% of women reproductive age. Several thrombophilic risk factors might contribute RPL.To investigate relationship between novel C20068T gene variant in the 3` end prothrombin and RPL, we tested 153 with RPL 111 controls for presence this variant. In patients, have detected four heterozygous (2.61%) no homozygous carriers. controls, carriers were detected. Our results indicate higher prevalence but difference was not...

AbstractObjective Pulmonary embolism is usually considered as a complication of deep vein thrombosis, but there are still number cases isolated pulmonary embolism. We aimed to investigate whether prothrombin 3' end gene variants might play signifi cant role in the pathogenesis embolism.Methods and results In this study 100 patients with controls were screened by DNA sequencing. Screening included last intron, exon, 3'UTR part 3'FR region gene. Our have shown that heterozygous carriers FII...

The link between thrombotic disorders and cancer has been known for over 150 years, although the precise mechanism of this relationship not yet resolved. Current data show that thrombin a significant role in metabolism, invasiveness, adhesion survival. However, regarding expression precursor prothrombin various cell lines are scarce. Therefore, it was our objective to determine whether common cancer-derived (Caco-2, MCF-7, SK-BR-3, U-87 U-251) express prothrombin. RNA level assessed by qPCR,...