A5072916100- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Insect Resistance and Genetics
- Sirtuins and Resveratrol in Medicine
- Biochemical effects in animals
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Phytochemical compounds biological activities
- Pharmacological Effects of Natural Compounds
- Metal Extraction and Bioleaching
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Chromium effects and bioremediation
- Circular RNAs in diseases
- Environmental remediation with nanomaterials
- Cancer-related molecular mechanisms research
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Mosquito-borne diseases and control
- Plant Micronutrient Interactions and Effects
- Protein Hydrolysis and Bioactive Peptides
Osmania University
2015-2023
University of Hyderabad
2015-2022
Centurion University of Technology and Management
2021-2022
Roswell Park Comprehensive Cancer Center
2011-2019
Therapeutics Clinical Research
2011
Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of protein (XIAP)-mediated translocation Bax mitochondria where it underwent oligomerization initiate apoptosis. Resveratrol treatment promoted interaction between and XIAP cytosol on mitochondria, suggesting plays critical role activation mitochondria....
Previous studies have demonstrated that curcumin induces mitochondria-mediated apoptosis. However, understanding of the molecular mechanisms underlying curcumin-induced cell death remains limited. In this study, we demonstrate treatment cancer cells caused dose- and time-dependent caspase-3 activation, which is required for apoptosis as confirmed using pan caspase inhibitor, z-VAD. Knockdown experiments knockout excluded a role caspase-8 in activation. contrast, Apaf-1 deficiency or...
Azadirachta indica, commonly known as neem, has a wide range of medicinal properties. Neem extracts and its purified products have been examined for induction apoptosis in multiple cancer cell types; however, underlying mechanisms remain undefined. We show that neem oil (i.e., neem), which contains majority limonoids including azadirachtin, induced apoptotic autophagic death. Gene silencing demonstrated caspase cascade was initiated by the activation caspase-9, whereas caspase-8 also...
In the intrinsic death pathway, cytochrome C (CC) released from mitochondria to cytosol triggers Apaf-1 apoptosome formation and subsequent caspase activation. This process can be recapitulated using recombinant CC in presence of nucleotides ATP or dATP [(d)ATP] fresh without need exogenous nucleotides. Surprisingly, we found that stored cytosols failed support CC-initiated Storage at different temperatures led loss all (deoxy)nucleotides including (d)ATP. Addition (d)ATP such partially...
MiRNAs are 20-22 nucleotide long single-stranded non-coding RNA sequences, which can regulate post transcriptional activity of mRNA by binding with it at 3’UTR region (untranslated region). Thus deregulation miRNA expression is responsible for dysregulating function contributes in developing various diseases as well cancerous phenotypes. Alteration single sequence one the reasons behind expression. The most frequent carcinoma current day breast cancer causes a high mortality among...
Abstract Proapoptotic BH3-only protein Bim plays an important role in Bax/Bak-mediated cytochrome c release and apoptosis. Here we provide evidence for a novel prosurvival function of cancer cells. was constitutively overexpressed multiple prostate breast cells as well primary tumor Quantitative real time PCR analysis showed that transcriptionally upregulated. We have identified eight endogenous E2F1-binding sites on the promoter using silico analysis. Luciferase assay demonstrated...
Proapoptotic BH3‐only protein Bim plays an important role in Bax/Bak‐mediated cytochrome c release and apoptosis. Here we provide evidence for a novel prosurvival function of cancer cells. was constitutively overexpressed multiple prostate breast cells as well primary tumor Quantitative real time PCR analysis showed that transcriptionally upregulated. We have identified eight endogenous E2F1‐binding sites on the promoter using silico analysis. Luciferase assay demonstrated expression...
FAD Synthetase (FADS) [EC 2.7.7.2], the second enzyme in flavin cofactor biosynthetic pathway converts FMN to FAD, plays an important role many redox reactions. Neurospora crassa FADS (NcFADS) was cloned and overexpressed E. coli cells. Recombinant NcFADS purified high yields of ∼8 mg per liter bacterial culture using a single step glutathione sepharose affinity chromatography. SDS-PAGE MALDI-MS revealed that has molecular mass ∼31 kDa. Enzyme kinetic analysis monitored by reverse phase HPLC...