A5042088140- Metabolomics and Mass Spectrometry Studies
- Medical Imaging Techniques and Applications
- Glioma Diagnosis and Treatment
- Chemical Reactions and Isotopes
- Gut microbiota and health
- Analytical Chemistry and Sensors
- Analytical Chemistry and Chromatography
- Molecular Junctions and Nanostructures
- Advanced MRI Techniques and Applications
- Electrochemical sensors and biosensors
- Radiomics and Machine Learning in Medical Imaging
- Advanced biosensing and bioanalysis techniques
- Chemical Reaction Mechanisms
- Spectroscopy and Chemometric Analyses
- Synthesis and Properties of Aromatic Compounds
- Molecular Sensors and Ion Detection
- Radiation Dose and Imaging
- Chemical Synthesis and Reactions
- Radical Photochemical Reactions
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Advanced Chemical Physics Studies
- Gene expression and cancer classification
- Cerebrovascular and Carotid Artery Diseases
- Mass Spectrometry Techniques and Applications
Kubota (Japan)
2019-2025
Institute of Brain and Blood Vessels
2019-2025
Nagoya University
2024
Research Institute for Brain and Blood Vessels Akita
2023
Osaka University
1993-2023
Hirosaki University
2021
Kagawa University
2014-2019
Kyoto University
1977-2019
Kurashiki Central Hospital
2015
Ajinomoto (Japan)
2015
Metabolic microenvironment of tumor cells is influenced by oncogenic signaling and tissue-specific metabolic demands, blood supply, enzyme expression. To elucidate tumor-specific metabolism, we compared the metabolomics normal tissues surgically resected pairwise from nine lung seven prostate cancer patients, using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Phosphorylation levels enzymes involved in central carbon metabolism were also quantified. Metabolomic...
Principal component analysis (PCA) has been widely used to visualize high-dimensional metabolomic data in a two- or three-dimensional subspace. In metabolomics, some metabolites (e.g., the top 10 metabolites) have subjectively selected when using factor loading PCA, and biological inferences are made for these metabolites. However, this approach may lead biased because not objectively with statistical criteria. We propose procedure that selects hypothesis testing of PCA makes about...
Background Plasma amino acid concentrations vary with various diseases. Although reference intervals are useful in daily clinical practice, no have been reported for plasma acids a large Japanese population. Methods Reference individuals were selected from 7685 subjects examined the Ningen Dock 2008. A total of 1890 based on exclusion criteria, and samples after outlier each concentration excluded. The lower limit was set at 2.5th percentile upper 97.5th percentile. Results By use nested...
PURPOSE: To evaluate the effectiveness of positron emission tomography (PET) with carbon-11 choline in brain tumor imaging. MATERIALS AND METHODS: A rat glioma cell line (C6) was incubated C-14 choline; time course uptake and metabolism determined vitro. C-11 injected intravenously tumor-bearing rats; distribution organs determined. also 20 patients (aged 6-86 years) tumors two volunteers 38 58 years); tracer Regional cerebral blood flow measured by using oxygen-15 water on same day....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReduction by a model of NAD(P)H. 30. Proof for the electron-proton-electron-transfer mechanismAtsuyoshi Ohno, Tohru Shio, Hiroyuki Yamamoto, and Shinzaburo OkaCite this: J. Am. Chem. Soc. 1981, 103, 8, 2045–2048Publication Date (Print):April 1, 1981Publication History Published online1 May 2002Published inissue 1 April 1981https://pubs.acs.org/doi/10.1021/ja00398a028https://doi.org/10.1021/ja00398a028research-articleACS PublicationsRequest reuse...
Abstract The driving force for the catalytic activity of metal ions on reduction α-keto esters with an NAD(P)H-model compound has been discussed. scope reaction and spectroscopic investigations as well molecular orbital consideration have revealed that transition state consists a ternary complex in analogy coenzyme–enzye–substrate enzymic system. It is concluded that, at state, one electron migrates from model to substrate through ion, which followed by transfer proton.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReduction by a model of NAD(P)H. 29. Kinetics and isotope effects for the reduction substituted trifluoroacetophenoneAtsuyoshi Ohno, Hiroyuki Yamamoto, Shinzaburo OkaCite this: J. Am. Chem. Soc. 1981, 103, 8, 2041–2045Publication Date (Print):April 1, 1981Publication History Published online1 May 2002Published inissue 1 April 1981https://doi.org/10.1021/ja00398a027RIGHTS & PERMISSIONSArticle Views238Altmetric-Citations68LEARN ABOUT THESE...
Metabolome analysis using capillary electrophoresis (CE) coupled with high-resolution mass spectrometry (HRMS) has the potential to improve coverage of metabolite detection because its high selectivity and sensitivity. Configuration interface between CE HRMS meet ground connection is essential for enabling independent regulation electrical currents in electrospray field. In present study, we applied an electrospray-ionization adapter equipped a grounded nebulizer CE-HRMS tested analytical...
This study sought to characterize the plasma metabolite profiling of patients with major depressive disorder (MDD).Psychiatric assessments were made Structured Clinical Interview for DSM-IV Axis I Disorders. In exploratory cohort, profiles 34 MDD and 31 mentally healthy controls compared using capillary electrophoresis-mass spectrometry. Among candidate metabolites, we focused on a showing largest difference. The absolute concentrations measured in two cohorts from psychiatric primary care...
Positron emission tomography (PET) with 15O-gas for quantifying cerebral blood flow (CBF) and oxygen metabolism is the gold standard assessing hemodynamics in ischemic cerebrovascular disease. However, conventional PET methods do not provide information on regional arrival timing, a hemodynamic parameter typically measured using computed (CT) perfusion contrast media. This study demonstrated that state-of-the-art clinical scanner optimized analysis can generate time maps. In this...
Glycyrrhiza uralensis, known for its diverse pharmacological effects, including immunoregulation, anti-tumor, and antioxidant properties, is a widely used medicinal plant found in more than 70% of traditional herbal medicines (Kampo) Japan. Although over 300 compounds have been discovered G. the molecular mechanisms underlying bioactivity remain largely unknown due to chemical diversity compounds. Here, we performed multiomics analysis incorporating untargeted hydrophilic metabolomics,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-Assembled Monolayers as Novel Biomembrane Mimetics. 1. Characterization of Cytochrome c Bound to Self-Assembled on Silver by Surface-Enhanced Resonance Raman SpectroscopyYasushi Maeda, Hiroyuki Yamamoto, and Hiromi KitanoCite this: J. Phys. Chem. 1995, 99, 13, 4837–4841Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March...
16S rRNA gene amplicon sequencing analysis (16S sequencing) has provided considerable information regarding the ecology of intestinal microbiome. Recently, metabolomics been used for investigating crosstalk between microbiome and host via metabolites. In present study, we determined accuracy with which data at different classification levels correspond to metabolome an in-depth understanding environment. Over 200 metabolites were identified using capillary electrophoresis time-of-flight mass...
Complexation of phthalic acid esters (PAEs) by a self-assembled monolayer (SAM) thiolated alpha-cyclodextrin (6-(2-mercaptoethylamino)-6-deoxy-alpha-cyclodextrin, MEA-alpha-CD) on gold electrode was examined cyclic voltammetry using hydroquinone (HQ) as probe. From the inhibitory effect phthalate inclusion HQ surface-confined cyclodextrin (CD), association constants (Kasn) with immobilized CD were estimated. For comparison, PAEs free alpha-CD spectrophotometrically methyl orange It concluded...
Self-assembled monolayer (SAM) of a thiolated cyclodextrin (CD) derivative was prepared, and stereoselective inclusional complexation optically active azo dyes (p- or o-methyl red conjugates with chiral 1-phenylethylamine, p- o-MR−PEA) both free CD the SAM on silver surface investigated by using resonance Raman (RR) surface-enhanced spectroscopy (SERRS), respectively. Characteristic molecular vibrations functional groups MR−PEA were used to describe complexation. To our knowledge, this is...
Abstract Kinetics for the reduction of methyl benzoylformate (1) with N-α-methylbenzyl-1-propyl-1,4-dihydronicotinamide (2) in presence magnesium perchlorate acetonitrile at 25 °C has been studied. The reaction follows first-order 1, 2, and zero-order ion when [2]<<[1], [Mg(ClO4)2]. When [2]= [Mg(ClO4)2]<<[1], kinetics is best explained by Eqs. 6 7. results indicate that 2 form a complex, which reacts 1 rate-determining step. It concluded activates to promote...
Abstract Background Osteoarthritis (OA) is one of the costliest and most disabling forms arthritis, it poses a major public health burden; however, its detailed etiology, pathophysiology, metabolism remain unclear. Therefore, purpose this study was to investigate key plasma metabolites metabolic pathways, especially focusing on radiographic OA severity synovitis, from large sample cohort study. Methods We recruited 596 female volunteers who participated in Iwaki Health Promotion Project...
Capillary electrophoresis coupled with tandem mass spectrometry (CE-MS/MS) offers advantages in peak capacity and sensitivity for metabolic profiling owing to the electroosmotic flow-based separation. However, utilization of data-independent MS/MS acquisition (DIA) is restricted due absence an optimal procedure analytical chemistry its related informatics framework. We assessed spectral quality using two DIA techniques, namely, all-ion fragmentation (AIF) variable (vDIA), isolate 60–800 Da...
Abstract Objective In quantitative positron emission tomography (PET) of the brain, partial volume effect due mainly to finite spatial resolution PET scanner (> 3 mm full width at half maximum [FWHM]) is a primary source error in measurement tracer uptake, especially small structures such as cerebral cortex (typically < thickness). The aim this study was evaluate correction (PVC) performance point spread function-incorporated reconstruction (PSF reconstruction) combination with latest...