The concentration of leukaemia inhibitory factor (LIF) was measured in uterine flushings obtained from normal fertile women, women with unexplained infertility and who suffered recurrent miscarriage. In LIF not detected on days luteinizing hormone (LH)+0 to LH+6 the cycle, but concentrations gradually increased day LH+7 a maximum at LH+12. amount significantly lower than those LH+10 (P < 0.05). production by cultured human epithelial stromal cells also investigated. detectable supernatants...

Previous studies indicate that eating behaviours and food cravings are associated with increased BMI obesity. However, the interaction between these other variables such as age, sex, genetics is complex. This study aimed to investigate relationships cravings, examine influence of body mass index (BMI) fat obesity-associated (FTO) genotype on relationships. A total 475 participants (252 female, 223 male, BMI: 25.82 ± 6.14 kg/m², age: 30.65 14.20 years) completed revised 18-question version...

Abstract Outcomes following SARS-CoV-2 infection are variable; whilst the majority of patients recover without serious complications, a subset develop prolonged illness termed Long COVID or post-acute sequelae (PASC). The pathophysiology underlying remains unclear but appears to involve multiple mechanisms including persistent inflammation, coagulopathy, autoimmunity, and organ damage. Studies suggest that microclots, also known as fibrinaloids, play role in COVID. In this context, we...

People with Long Covid (Post Covid-19 Condition) describe multiple symptoms which vary between and within individuals over relatively short time intervals. We aimed to the real-time associations different physical activity at individual patient level.Intensive longitudinal study of 82 adults self-reported (median duration 12-18 months). Data collection involved a smartphone app 5 daily entries 14 days continuous wearing wrist accelerometer. items included 7 (Visual Analog Scales) perceived...

The Post Covid-19 Condition (commonly known as Long Covid) has been defined by the World Health Organisation occurring in individuals with a history of probable or confirmed SARS CoV 2 infection, usually within 3 months from onset acute infection symptoms that last for at least two which cannot be explained an alternative diagnosis. Covid is associated over hundred recognised and affects tens millions people worldwide. Widely reported reductions quality life(QoL) functional status are caused...

Individual variability and inadequate response of negative symptoms are major limitations antipsychotic treatment in schizophrenia. A functional polymorphism, rs6295, the 5-HT1A-receptor gene (HTR1A) contributes to this symptom response. The DNA sequence containing rs6295 is rich cytosine methylation (CpG) sites; CpG an epigenetic factor that, like can modify transcriptional control. To investigate whether influences treatment, we determined at sites close from 82 Chinese subjects with a...

Abstract Importance Noncommunicable diseases are the leading cause of morbidity and mortality globally. Physical therapists working in private practice have an opportunity to embed health screening their support chronic disease prevention. However, it is not known if feasible conduct screenings physical therapist settings. Objective The aim this study was determine feasibility conducting client with point-of-care testing. Design This a study, comprising in-depth interviews descriptive...

Abstract: Huntington’s disease (HD) is associated with an expansion in the CAG repeat sequence of a gene on chromosome 4, resulting neurodegenerative process particularly affecting striatum and profound but selective changes content various neurotransmitters. Recently, transgenic mice expressing fragment human HD containing large have been generated; these exhibit progressive neurological phenotype that includes motor disturbances, as well neuronal deficits. To investigate their underlying...

The concentrations of endometrial proteins PP14 and CA-125 were measured in uterine flushings taken on days LH+10 LH+12 (10 12 after luteinizing hormone surge) the menstrual cycle from 15 normal, fertile women 49 who suffered recurrent miscarriage. concentration was significantly lower miscarriage patients than those controls both day (median: 1300, range: 3-10 300 ng/ml versus median: 13 933, 2174-40 404 ng/ml; P < 0.01) 1560, 820-12 100 14 047, range 1402-62 108 0.05). Similarly compared...

The Parkinson's disease-associated protein α-synuclein exhibits significant conformational heterogeneity. Bacterially expressed is known to bind copper, resulting in the formation of aggregation-prone compact conformations. However, vivo, undergoes acetylation at its N-terminus. Here effect this modification and pathological H50Q mutation on copper binding subsequent transitions were investigated by electrospray ionization-ion mobility spectrometry-mass spectrometry. We demonstrate that...

ABSTRACT Extensive loss of dopaminergic neurons and aggregation the protein α-synuclein into ubiquitin-positive Lewy bodies represents a major neuropathological hallmark Parkinson's disease (PD). At present, generation large nuclear-associated from endogenous wild-type α-synuclein, translationally regulated under its own promoter in human cell culture models, requires costly time-consuming protocols. Here, we demonstrate that fully differentiated SH-SY5Y neuroblastoma cells grown...

We investigated GRIN1 and GRIN2B promoter methylation in first-episode schizophrenia patients compared with siblings controls, testing for correlations between DNA methylation, cognitive performance clinical variables.Blood-derived from all groups underwent bisulfite conversion pyrosequencing to determine at CpG sites within the promoters results were measure of global LINE-1.We found hypomethylation among CpGs analyzed greater LINE-1 siblings. CpG4 was correlated a intellectual...

Misfolding and aggregation of α-synuclein (α-syn) into Lewy bodies is associated with a range neurological disorders, including Parkinson's disease (PD). The cell-to-cell transmission α-syn pathology has been linked to soluble amyloid oligomer populations that precede body formation. Oligomers produced in vitro under certain conditions have demonstrated induce intracellular cell culture models. In the present study, we characterize, by ESI-ion mobility spectrometry (IMS)-MS, specific...

Deficits of brain parvalbumin (PV) are a consistent finding in schizophrenia and models psychosis. We investigated whether this is associated with abnormal PV gene (PVALB) methylation the schizophrenia. Bisulfite pyrosequencing was used to determine cytosine (CpG) PVALB promoter sequence. Greater found hippocampus, while no differences were observed prefrontal cortex. LINE-1 methylation, measure global also elevated both regions schizophrenia, although change independent effect. These...

To determine the prevalence of metabolic syndrome in clients presenting for primary care physiotherapy within private practice settings, and factors that may be associated with syndrome. The secondary aim was to client's attitudes towards lifestyle change.

ABSTRACT Objective To determine the variation in concentration of endometrial protein PP14 uterine flushings throughout menstrual cycle comparing this to concentrations plasma samples. Design Precise timing all samples by luteinising hormone surge. Setting Jessop Hospital for Women, Sheffield. Subjects Twenty‐three regularly cycling, previously fertile volunteer women. Interventions Observational study; 10 ml physiological saline was used flush cavity once or serially study. Main outcome...

Journal Article The measurement of CA 125 and placental protein 14 in uterine flushings women with recurrent miscarriage; relation to endometrial morphology Get access C.F. Dalton, Dalton 5 1Biomedical Research Unit, Jessop Hospital for WomenLeavygreave Road, Sheffield S3 7RE 5To whom correspondence should be addressed Search other works by this author on: Oxford Academic PubMed Google Scholar S.M. Laird, Laird 2Division Biomedical Sciences/Health Institute, Hallam UniversityPond Street, SI...

Parkinson's disease is the second most common neurodegenerative disorder, in which both alpha-synuclein (α-syn) and dopamine (DA) have a critical role. α-Syn known to be natively unstructured equilibrium with subpopulations of more compact structures. It these structures that are thought linked amyloid formation. In presence DA, α-syn yields diverse range SDS-resistant, non-amyloid oligomers, however precursor state conformation has not been established. Here, three DA molecules observed...

Abstract Background Polycystic ovary syndrome (PCOS) affects up to 20% of women and is characterised by higher amounts visceral fat, obesity, insulin resistance, dyslipidemia reproductive cardiometabolic complications. Increased oxidised low-density lipoprotein (LDL) concentrations have been associated with an increased risk cardiovascular disease (CVD)-related events. Oxidised LDL rarely used as a marker for CVD in PCOS-related studies despite its widely accepted role atherogenesis the...