A5037019694- Alzheimer's disease research and treatments
- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Parkinson's Disease Mechanisms and Treatments
- Advanced biosensing and bioanalysis techniques
- Organometallic Complex Synthesis and Catalysis
- Ion-surface interactions and analysis
- X-ray Diffraction in Crystallography
- Metabolomics and Mass Spectrometry Studies
- Crystallization and Solubility Studies
- Ferrocene Chemistry and Applications
- Crystallography and molecular interactions
- Advanced Proteomics Techniques and Applications
- Plant Water Relations and Carbon Dynamics
- Experimental Learning in Engineering
- Prion Diseases and Protein Misfolding
- Metal complexes synthesis and properties
- Innovative Teaching Methods
- RNA Interference and Gene Delivery
- Lanthanide and Transition Metal Complexes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Enzyme Structure and Function
- Science Education and Pedagogy
- Cholinesterase and Neurodegenerative Diseases
- Rangeland and Wildlife Management
Sheffield Hallam University
2015-2024
Children's Hospital of Philadelphia
2024
Saybrook University
2020
University of North Carolina at Chapel Hill
2018
Agricultural Research Service
2010-2017
University of Hull
2017
University of Chicago
2005-2017
Lawrence Berkeley National Laboratory
1995-2014
Dublin City University
2011-2013
Morehead State University
2012-2013
The energy distributions of positive ions ejected from polycrystalline Mo and Ni by He+, Ne+, Ar+ primary in the range 0.5 to 3.0 keV have been found consist a low-energy distribution characteristic sputtered sharp peak at higher energy. These high-energy are shown be which experienced single two-body collisions with surface atoms. Multiply-charged were not detected. It was that, approximately, monolayer adsorbed gas sufficient prevent detection incident scattered metal target He+ used...
Detailed knowledge of the tertiary and quaternary structure proteins protein complexes is immense importance in understanding their functionality. Similarly, variations conformational states form underlying mechanisms behind many biomolecular processes, numerous which are disease-related. Thus, availability reliable accurate biophysical techniques that can provide detailed information concerning these issues paramount importance. Ion mobility spectrometry (IMS) coupled to mass (MS) offers a...
Dopamine (DA) and alpha-synuclein (alpha-SN) are two key molecules associated with Parkinson's disease (PD). We have identified a novel action of DA in the initial phase alpha-SN aggregation demonstrate that induces to form soluble, SDS-resistant oligomers. The DA:alpha-SN oligomeric species not amyloidogenic as they do react thioflavin T lack typical amyloid fibril structures visualized electron microscopy. Circular dichroism studies indicate presence lipid membranes interacts alpha-SN,...
Oscillations in the low-energy noble-gas ion-scattering cross sections versus primary ion energy have been observed for a number of solid surfaces. This behavior is ascribed to resonant electron exchange between electronic states surface atom and ion. The oscillations are sensitive particular levels chemical environment atom.
Amelyoid-beta peptide (Abeta) is a major causative agent responsible for Alzheimer's disease (AD). Abeta contains high affinity metal binding site that modulates aggregation and toxicity. Therefore, identifying molecules targeting this represents valid therapeutic strategy. To test hypothesis, range of L-PtCl(2) (L = 1,10-phenanthroline derivatives) complexes were examined shown to bind Abeta, inhibit neurotoxicity rescue Abeta-induced synaptotoxicity in mouse hippocampal slices....
The Amyloid beta peptide (Abeta) of Alzheimer's diseases (AD) is closely linked to the progressive cognitive decline associated with disease. Cu2+ ions can induce de novo aggregation Abeta into non-amyloidogenic aggregates and production a toxic species. mechanism by which mediates change from amyloid material toward induced poorly defined. Here we demonstrate that state Abeta1-42 at neutral pH governed Cu2+:peptide molar ratio. By probing content total aggregation, observed distinct...
Dementia with Lewy bodies (DLB) is pathologically characterized by the presence of α-synuclein-containing within neocortical, limbic, and paralimbic regions. Like Alzheimer's disease (AD), Aβ plaques are also present in most DLB cases. The contribution to development unclear. [ 11 C]-Pittsburgh compound B ([ C]-PIB) a thioflavin-T derivative that has allowed vivo burden be quantified using positron emission tomography (PET). C]-PIB PET studies have shown similar high cortical binding AD...
Abstract Net ecosystem productivity ( NEP ) was measured on shortgrass steppe SGS vegetation at the USDA C entral P lains E xperimental R ange in northeastern olorado from 2001 to 2003. Large year‐to‐year differences were observed annual , with >95% of net carbon uptake occurring during M ay and J une. Low precipitation 2002 A pril une time period greatly reduced uptake. events (>10 mm day −1 promoted uptake, while small (<10 enhanced heterotrophic respiration resulted a loss...
The key to understanding amyloid disease is the characterization of oligomeric species formed during early stages fibril assembly. Here we have used electrospray ionisation-ion mobility spectrometry-mass spectrometry identify and structurally characterize oligomers assembly from β 2 -microglobulin ( m). m are shown collision cross-sections consistent with monomeric units arranged in elongated assemblies prior formation. Direct observation, separation, quantification transient reveals that...
Abstract Outcomes following SARS-CoV-2 infection are variable; whilst the majority of patients recover without serious complications, a subset develop prolonged illness termed Long COVID or post-acute sequelae (PASC). The pathophysiology underlying remains unclear but appears to involve multiple mechanisms including persistent inflammation, coagulopathy, autoimmunity, and organ damage. Studies suggest that microclots, also known as fibrinaloids, play role in COVID. In this context, we...
Dissociation of human β-2-microglobulin (β 2 m) from the heavy chain class I HLA complex is a critical first step in formation amyloid fibrils this protein. As consequence renal failure, concentration circulating monomeric β m increases, ultimately leading to deposition protein into and development disorder, dialysis-related amyloidosis. Here we present crystal structure form determined at 1.8-Å resolution that reveals remarkable structural changes relative HLA-bound These involve...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBioorganometallic chemistry. 1. Synthesis, structure, and selectivity in reactions of a nucleobase several nucleosides with (.eta.5-pentamethylcyclopentadienyl)rhodium aqua complexDavid P. Smith, Eduardo Baralt, Bruce Morales, Marilyn M. Olmstead, Marcos F. Maestre, Richard H. FishCite this: J. Am. Chem. Soc. 1992, 114, 26, 10647–10649Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December...
The precise mechanism of protein folding remains elusive and there is a deficiency biophysical techniques that are capable monitoring the individual behavior copopulated conformers during process. Herein, an ion mobility spectrometry (IMS) device integrated with electrospray ionization mass (ESI-MS) has been used to successfully separate analyze differing in cross section and/or charge state. In initial test, ensemble folded partially cytochrome c was separated. A detailed study undertaken...
Mass spectrometry imaging (MSI) is an established analytical tool capable of defining and understanding complex tissues by determining the spatial distribution biological molecules. Three-dimensional (3D) cell culture models mimic pathophysiological environment in vivo tumors are rapidly emerging as a valuable research tool. Here, multimodal MSI techniques were employed to characterize novel aggregated 3D lung adenocarcinoma model, developed group tissue. Regions tumor heterogeneity hypoxic...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRegioselective carbon-nitrogen bond cleavage in an .eta.2(N,C)-coordinated pyridine and .eta.1(N) .fwdarw. .eta.2(N,C) bonding rearrangment coordinated quinoline: models for hydrodenitrogenation catalysisSteven D. Gray, David P. Smith, Michael A. Bruck, E. WigleyCite this: J. Am. Chem. Soc. 1992, 114, 13, 5462–5463Publication Date (Print):June 1, 1992Publication History Published online1 May 2002Published inissue 1 June...