A5036737477- Tuberculosis Research and Epidemiology
- Cancer therapeutics and mechanisms
- Glycosylation and Glycoproteins Research
- Antibiotic Resistance in Bacteria
- Congenital heart defects research
- RNA modifications and cancer
- Mycobacterium research and diagnosis
- Galectins and Cancer Biology
- Genomic variations and chromosomal abnormalities
- Congenital Diaphragmatic Hernia Studies
- Immunodeficiency and Autoimmune Disorders
- Tracheal and airway disorders
- Antibiotics Pharmacokinetics and Efficacy
- Congenital Heart Disease Studies
- Inflammatory Bowel Disease
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Genomics and Rare Diseases
- Monoclonal and Polyclonal Antibodies Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Gene expression and cancer classification
- dental development and anomalies
- Carbohydrate Chemistry and Synthesis
- Cancer-related gene regulation
- Single-cell and spatial transcriptomics
Université de Lille
2016-2024
Centre Hospitalier Universitaire de Lille
2012-2024
Maladies RAres du DEveloppement embryonnaire et du MEtabolisme
2016-2024
Inserm
2011-2022
Centre National de la Recherche Scientifique
2004-2022
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2021
Hôpital Jeanne de Flandre
2017
Lille’s Cardiology Hospital
2017
Université de Bordeaux
2015
Institut Européen de Chimie et Biologie
2015
The mucin MUC4 and its membrane partner the ErbB2 oncogenic receptor are potential interacting partners in human pancreatic tumour development. However, way they function is still largely unknown. In this work, we aimed to identify cellular mechanisms intracellular signalling pathways under control of both a adenocarcinomatous cell line. Using co-immunoprecipitation GST pull-down, show that interact line CAPAN-2 via EGF domains MUC4. Stable clones were generated which either or knocked down...
Abstract Pancreatic cancer cells express high levels of MUC1, MUC4 and MUC16 mRNAs that encode membrane-bound mucins. These share unusual features such as a long half-life. However, it remains unknown how mucin mRNA stability is regulated. Galectin-3 (Gal-3) an endogenous lectin playing important biological functions in epithelial cells. Gal-3 encoded by LGALS3 which up-regulated pancreatic cancer. Despite the absence RNA-recognition motif, interacts indirectly with pre-mRNAs nucleus...
In an attempt to investigate the molecular basis of pyrazinamide hydrolysis by PncA protein from Mycobacterium tuberculosis, we determined pyrazinamidase activity nine mutants bearing a single amino acid substitution. Among them, three (D8G, K96T and S104R) had virtually no (< or =0.004 unit/mg), five (F13S, T61P, P69L, Y103S A146V) retained low level (0.06-0.25 unit/mg) one (T167L) exhibited wild-type (1.51 units/mg). The possible structural effects these substitutions were assessed...
Ethionamide is an antituberculous drug for the treatment of multidrug-resistant Mycobacterium tuberculosis. This antibiotic requires activation by monooxygenase EthA to exert its activity. Production controlled transcriptional repressor EthR, a member TetR family. The sensitivity M. tuberculosis ethionamide can be artificially enhanced using synthetic ligands EthR that allosterically inactivate DNA-binding Comparison several structures co-crystallized with various suggested structural...
The NOD2 gene, involved in innate immune responses to bacterial peptidoglycan, has been found be closely associated with Crohn's Disease (CD), an Odds Ratio ranging from 3⁻36. Families three or more CD-affected members were related a high frequency of gene variations, such as R702W, G908R, and 1007fs, reported the EPIMAD Registry. However, some rare CD multiplex families described without identification common linked-to-disease variations. In order identify new genetic variation(s) linked...
Objective We previously described a family in which predisposition to pheochromocytoma (PCC) segregates with germline heterozygous KIF1B nucleotide variant (c.4442G>A, p.Ser1481Asn) three generations. During the clinical follow-up, one proband’s brother, negative for variant, developed bilateral PCC at 31 years. This prompted us reconsider genetic analysis. Design and methods Germline DNA was analyzed by next-generation sequencing (NGS) using multi-gene panel plus MLPA or whole exome...
The aim of the present study was to understand context and psychological impact for patients diagnosed with hereditary hemorrhagic telangiectasia (HHT). Semi-structured interviews were conducted 9 affected by HHT, transcripts analyzed using interpretative phenomenological analysis. results this allowed us propose a new hypothesis explain delay in diagnosis: trivialization symptoms associated HHT. Moreover, showed that genetic diagnosis HHT emotional shock, uncertainty about future, worry...
The HER2 receptor and its MUC4 mucin partner form an oncogenic complex via extracellular region of encompassing three EGF domains that promotes tumor progression pancreatic cancer (PC) cells. However, the molecular mechanism interaction remains poorly understood. Herein, we decipher at level role impact MUC4EGF in mediation binding affinities with PC cell tumorigenicity. We used integrative approach combining vitro bioinformatic, biophysical, biochemical, biological approaches, as well vivo...
Ribosome biogenesis in eukaryotes is a complex and highly orchestrated process involving more than 200 accessory factors addition to ribosomal RNAs proteins. Among the many involved, Sqt1p has been reported specifically bind uL16 act as chaperone. The crystal structure of full-length from yeast Saccharomyces cerevisiae solved at 3.35 Å resolution. A SAD experiment Se K edge an S-SAD on same selenomethionine-substituted protein allowed unambiguous positioning selenomethionine Cys residues. On...
Abstract Topologically Associating Domains (TAD)-boundaries induce spatial constraints, allowing interaction between regulatory elements and promoters only within their TAD. Their disruption could lead to disease, through gene-expression deregulation. This mechanism has been shown in a relatively low number of diseases proportion patients, raising the possibility TAD boundary without phenotypical consequence. We investigated, therefore, occurrence boundaries general population. Coordinates...
ABSTRACT Congenital diaphragmatic hernia (CDH) is a relatively common and genetically heterogeneous structural birth defect associated with high mortality morbidity. We describe eight unrelated families novel X-linked condition characterized by diaphragm defects, variable anterior body wall anomalies, and/or facial dysmorphism. Using linkage analysis whole exome or genome sequencing, we identified missense variants in the actin binding domains of plastin 3 ( PLS3 ), gene encoding an bundling...