A5044013990- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Drug-Induced Hepatotoxicity and Protection
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Dermatology and Skin Diseases
- Respiratory and Cough-Related Research
- Inhalation and Respiratory Drug Delivery
- Respiratory Support and Mechanisms
- Pharmaceutical Practices and Patient Outcomes
- Estrogen and related hormone effects
- Hormonal Regulation and Hypertension
- Congenital Diaphragmatic Hernia Studies
- Neonatal skin health care
- Computational Drug Discovery Methods
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Pharmaceutical studies and practices
- Pediatric health and respiratory diseases
- Pharmacovigilance and Adverse Drug Reactions
- Medical Malpractice and Liability Issues
- Advancements in Transdermal Drug Delivery
- Health Systems, Economic Evaluations, Quality of Life
- Patient Safety and Medication Errors
University of Oulu
2015-2024
Oulu University Hospital
2015-2024
Northern Ostrobothnia Hospital District
2020-2022
Borealis (Finland)
2020-2022
University of Tübingen
2008-2015
Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology
2008-2015
Helsinki University Hospital
2001-2011
ENT and Allergy
2006-2009
University of Turku
2005
University of Helsinki
1986-2001
HepaRG cells possess the unique property to differentiate in vitro and express various functions of mature hepatocytes, including major cytochromes P450 (P450s). In present study, we carefully analyzed mRNA expression activity P450s their responsiveness three prototypical inducers, phenobarbital, rifampicin, omeprazole, differentiated cell cultures over a 4-week period after low high seeding. Only minor differences were observed activities when measured by two cocktails probe substrates,...
Our objective was to study the effect of antiplatelet agents clopidogrel and ticlopidine on bupropion (INN, amfebutamone) hydroxylation, a probe reaction for cytochrome P450 (CYP) 2B6 activity.Twelve healthy male volunteers took single 150-mg oral dose either alone or after pretreatment with 75 mg once daily 250 twice 4 days. On day 4, administered. Plasma concentrations its CYP2B6-catalyzed metabolite, hydroxybupropion, were measured up 72 hours.The mean area under plasma concentration-time...
In order to study the determinants of respiratory system impedance and bronchodilator response in preschool children, a sample (n = 109) healthy children (age 2.1-7.0 years) attending kindergarten was measured by using impulse oscillometry. Their selection based on standardized questionnaire, negative skin prick test results clinical examination, sufficient cooperation. Triple measurements resistance (Rrs) reactance (Xrs) at 5, 10, 15 20 Hz, total (Zrs), resonance frequency (Fr) dependence...
The forced oscillation technique makes it possible to evaluate the mechanical properties of respiratory system with a minimum cooperation. method is therefore especially useful in children. Impulse oscillometry (IOS) commercially available version this technique. There is, as yet, limited information on reference values for IOS aim study was extend variables and their correlation height, weight age healthy A sample (n = 360) children (age 2.1-11.1 years) measured by using impulse (IOS;...
Aims: NADPH:CYP oxidoreductase (POR) is an essential component of several enzyme systems, including the microsomal CYP monooxygenases. We investigated genetic and nongenetic POR variability its impact on drug-oxidation activities in human liver microsomes. Material methods: mRNA, protein activity, as well ten major activities, were measured microsomes 150 Caucasian surgical samples. Matrix-assisted laser desorption/ionisation-time flight mass spectrometric assays established to determine...
Percutaneous absorption of hydrocortisone was studied in 18 children (aged from 6 weeks to 14½ years) with atopic or seborrhoeic dermatitis, by measuring their serum cortisol before and after application 1% cream. Endogenous secretion suppressed dexamethasone. A 24 h test performed on nine children. In six, percutaneous detected. The highest level reached within the first h. 4 developed basis test. This short children, eight them rise ranged 98 2669 nmol/1. 2 ACTH evaluate effect previous...
One major challenge in drug development is defining of the optimal animal species to serve as a model metabolism man. The study compared hepatic characteristics humans and six widely used experimental species. Classical vitro enzyme assays with known human cytochrome P450 (CYP) selectivity were employed optimized target CYP forms. profile activities best resembling was seen mouse followed by monkey, minipig, dog liver microsomes, rats displaying most divergent. widest interindividual...
<h3>Objective:</h3> To compare the effect of inhaled budesonide given daily or as-needed on mild persistent childhood asthma. <h3>Patients, design and interventions:</h3> 176 children aged 5–10 years with newly detected asthma were randomly assigned to three treatment groups: (1) continuous (400 μg twice for 1 month, 200 months 2–6, 100 7–18); (2) budesonide, identical group during 1–6, then exacerbations as needed 7–18; (3) disodium cromoglycate (DSCG) 10 mg times 1–18. Exacerbations...
The human drug metabolizing cytochrome P450 (CYP) 1A2, is one of the major isoforms contributing by about 5-20% to hepatic pool and catalyzing oxidative biotransformation up 10% clinically relevant drugs including clozapine caffeine. CYP1A2 activity interindividually highly variable although twin studies have suggested a high heritability, underlying genetic factors are still unknown. Here we adopted pathway-oriented approach using large liver bank (n=150) elucidate whether variants in...
Many carboxylic acid-containing drugs are associated with idiosyncratic drug toxicity (IDT), which may be caused by reactive acyl glucuronide metabolites. The rate of migration has been earlier suggested as a predictor reactivity. Additionally, Coenzyme A (CoA) conjugates known to reactive. Here, 13 acid moiety were incubated human liver microsomes produce for the determination half-lives and HepaRG cells monitor formation CoA conjugates, their further conjugate metabolites, trans-acylation...
Some inhibitory agents against CYP2B6 have been reported, but none of these has extensively characterized or compared with others, as to the potency and selectivity inhibition toward CYP2B6. The goal this work was find a selective potent chemical in vitro inhibitor using bupropion hydroxylation model reaction. At initial screening more than 30 substances, ticlopidine, triethylenethiophosphoramide (thioTEPA), metyrapone, xanthate C8, benzylisothiocyanate displayed IC<sub>50</sub> values...
The aim of this study was to determine the extent which bronchopulmonary dysplasia (BPD) affects diffusing properties lung tissue in childhood. Pulmonary function 31 prematurely born children (BW. < 1250 g) examined at ages 7–11 years. Twenty out met criteria for BPD. remaining 11 had milder forms neonatal disease. healthy same age and term served as a control group. capacity carbon monoxide (DLCO) measured by single breath method. Lung volumes were determined body plethysmograph expiratory...