The parasite species complex Anisakis simplex sensu lato ( stricto ; A. s.s .), pegreffii , C ) is the main cause of severe anisakiasis (allergy) worldwide and now an important health matter. In this study, relationship their allergenic capacities assessed by studying differences between two most frequent ., hybrid haplotype active L3 larvae parasiting Merluccius merluccius . They were compared 2D gel electrophoresis parallel Western blot (2DE gels hybridized with pools sera from patients)....

Abstract Forficula auricularia (the European earwig) is possibly a complex of cryptic species. To test this hypothesis, we performed: (1) phylogeographic study based on fragments the mitochondrial COI and nuclear ITS2 markers wide geographic sampling, (2) morphometric analyses lineages present in Spain (3) niche overlap analyses. We recovered five reciprocally monophyletic ancient phylogroups with unique historical patterns distribution, climatic niches diversification. External morphology...

Anisakis simplex sensu stricto and pegreffii are sibling species of nematodes parasitic on marine mammals. Zoonotic human infection with third stage infective larvae causes anisakiasis, a debilitating potentially fatal disease. These 2 show evidence hybridisation in geographical areas where they sympatric. How the their hybrids differ is still poorly understood.Third s.s., were sampled from Merluccius merluccius (Teleosti) hosts captured waters FAO 27 area. Specimens each distinguished...

A common strategy to limit the negative impact of biological invasions is control through release specialized alien natural enemies. However, plans are not without risks, which include parasitism native hosts and hybridization with related species, particularly those that potential enemies invasive species. Here, we evaluate these risks resulting from introduction parasitoid wasp Torymus sinensis (Hymenoptera: Torymidae) in Europe Asian chestnut gall Dryocosmus kuriphilus Cynipidae). We...

Abstract Flightless, phoretic insects of the tribe Meloini include several widespread species able to inhabit both mainland and oceanic continental islands. Relationships between non‐phoretic are unclear, precluding broad evolutionary biogeographic analyses within group. We provide a robust molecular phylogenetic framework for Meloini, with finer sampling in Western Palaearctic, by analysing two nuclear ( Wg , 18S) mtDNA cox1 16S) gene fragments 24 representing eight subgenera Meloe...

Exoskeletons of beetles and their associated morphological characteristics can serve many different functions, including thermoregulation. We study the thermal role exoskeleton in 13 Geotrupidae dung beetle species using heating experiments under controlled conditions. The main purpose was to measure influence sources (solar radiance vs. infrared), animal position (dorsal exposure ventral exposure), identity, phylogenetic relationships on internal asymptotic temperatures rates. response...

Abstract The molecular variability has been analysed at the mitochondrial level in 288 honeybee colonies from 28 localities distributed along four Catalonian provinces. In total sixteen different haplotypes belonging to three evolutionary lineages were found. west European M lineage found a high frequency provinces and is fixed Lérida, whereas African A shows cline of distribution being more frequent northern Catalonia. east C (one Gerona two Barcelona). These results are most likely...

Abstract The host range of parasitoids varies greatly among species: Some only parasitize one to a few hosts (specialists), while others multiple species or variety types (generalists). direction most shifts in parasitoid groups, that is from generalist specialist or, alternatively, generalist, unknown. To explore the origin shifts, we studied clade within genus Torymus (Hymenoptera, Chalcidoidea) includes both and Cynipidae (Hymenoptera). We analysed phylogenetic relationships on basis two...

Male cerci variability was used as the main source of information to separate taxonomic units in genus Pseudochelidura. Based on these data it considered that three species Pseudochelidura coexisted Pyrenees: P. sinuata, minor and montuosa. However, our phylogeographic phylogenetic analyses based molecular (mitochondrial cytb nuclear ITS2) do not support those conclusions. Combining with morphological studies we concluded that: 1) Pyrenees are inhabited by a single evolutionary unit: sinuata...

Since the description of Eulithinus analis (Rambur, 1838) taxonomic position this Sierra Nevada endemic earwig has been controversial. It subdivided in different taxa, assigned to various genera or transferred subfamilies. With aim clarifying its systematics, we performed a mitochondrial phylogeographic analysis using specimens from localities representing diverse phenotypes treated as differentiated taxa until now, and phylogenetic including representatives apparently distant, but...

SUMMARYThe mitochondrial DNA of 260 honey bee (Apis mellifera) colonies located in eastern Spain was characterized through the RFLP analysis intergenic tRNAleu-COII region. In total, 16 different haplotypes were found, eight which belong to African evolutionary lineage and west European. The results indicate a high level genetic diversity this haplotype frequency is congruent with north-east-south-west gradient extending across whole Iberian peninsula. Several beekeeping techniques...

Abstract Changes in life history traits are often considered speciation triggers and can have dramatic effects on the evolutionary of a lineage. Here, we examine consequences changes two traits, host‐type phoresy, hypermetamorphic blister beetles, Meloidae. Subfamilies Nemognathinae Meloinae exhibit complex cycle involving multiple metamorphoses parasitoidism. Most genera tribes bee‐parasitoids, include phoretic or nonphoretic species, while feed grasshopper eggs. These different strategies...

Abstract Anostraca are known by their ability for long-distance dispersal, but the existence in several species of deep, geographically structured mtDNA lineages suggests populations subjected to allopatric differentiation, isolation, and prevalence local scale dispersion. Tanymastix stagnalis is one most widespread previous studies revealed an unclear geographical pattern genetic diversity. Here, we analyze from Iberian Italian Peninsulas, Central Europe, Scandinavia, with aim characterize...

The phylogenetic placement of Jekelius brullei (Jekel, 1866) and J. punctulatus (Coleoptera: Geotrupidae) was assessed using mitochondrial nuclear molecular data to discern contrasting nomenclatural views provided by López-Colón (1996) the Catalogue Palaearctic Coleoptera (Löbl et al. 2006). Our results support both monophyletic classification status genus López-Colón, 1989; splitting into subgenera 1989 Reitterius 1996. basal these two species also suggests an oriental origin for within...

Abstract Aim Discerning the relative role of geographical and ecological factors in promoting diversification is central to our understanding origin maintenance biodiversity. We explore roles geology tolerance a group wingless beetles with low dispersal potential. Location Western Mediterranean (Iberian Peninsula North Africa). Taxon Darkling (Tenebrionidae: Misolampus ). Methods sequenced nine gene fragments from mitochondrial nuclear genomes all extant species reconstruct their phylogeny,...

The Pyrenees are inhabited by scattered populations of earwigs the genus Chelidura Latreille, 1825. There is some controversy about specific assignment these populations: while most authors assign them to C. pyrenaica (Gené, 1832), other consider that aptera (Mégerlé, 1825) also present in Pyrenees. main objective this work was revise identity and synonyms Pyrenean . Specimens from recent fieldwork collections (MNCN-CSIC) were used for morphological molecular studies (cytochrome oxidase 1)....

L3 larvae of anisakid nematodes are an important problem for the fisheries industry and pose a potential risk human health by acting as infectious agents causing allergies vectors pathogens microrganisms. In spite close bacteria–nematode relationship very little is known anisakids microbiota. Fresh fish could be contaminated bacteria vectored in cuticle or intestine when migrate through muscles. As consequence, bacterial inoculum will spread, with effects on quality fish, possible clinical...

Phylogeographic and paleoclimatic modelling studies have been combined to infer the role of Pleistocene climatic oscillations as drivers genetic structure distribution Mediterranean taxa. For European whip snake, Hierophis viridiflavus, previous based on depicted a low reliability in pattern past suitability across central Basin, which barely fits species’ structure. In this study, we phylogeographic tools improve our understanding biogeographic history H. particularly extending sampling...

Abstract Background : L3 larvae of anisakid nematodes are an important problem for the fisheries industry and pose a potential risk human health by acting as agents causing allergies vectors pathogens microrganisms. In spite close bacteria-nematode relationship very little is known Anisakids microbiote. Fresh fish could be contaminated bacteria vectored in cuticle or intestine when migrate through muscles. As consequence bacterial inoculum will spread, affecting quality fish, possible...

We report the discovery of blister beetle, Meloe coelatus Reiche, 1957, in Qatar. According to Catalogue Palaearctic Coleoptera there is no record for genus Qatar, although well represented other areas Arabian Peninsula. M. a rare species, that present along wide area Northern Africa and Western Asia. Our findings bridge current geographic gap known distribution species from Saudi Arabia Iran. believe fauna Qatar might be underestimated. A thorough field survey recommended order document...