A5031488979- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- 14-3-3 protein interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Biochemical Analysis and Sensing Techniques
- GABA and Rice Research
- Developmental Biology and Gene Regulation
- Marine Invertebrate Physiology and Ecology
- Marine Ecology and Invasive Species
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Botulinum Toxin and Related Neurological Disorders
- Neurological diseases and metabolism
- Nuclear Receptors and Signaling
- Genetic Neurodegenerative Diseases
IRCCS San Camillo Hospital
2023-2024
University of Padua
2020-2023
Parkinson's disease (PD) is a neurodegenerative, progressive without cure. To prevent PD onset or at least limit neurodegeneration, better understanding of the underlying cellular and molecular mechanisms crucial. Mutations in leucine-rich repeat kinase 2 (LRRK2) gene represent one most common causes familial PD. In addition, LRRK2 variants are risk factors for sporadic PD, making an attractive therapeutic target. have been linked to impaired alpha-synuclein (α-syn) degradation neurons....
Abstract The Excitatory Amino Acid Transporter 2 (EAAT2) accounts for 80% of brain glutamate clearance and is mainly expressed in astrocytic perisynaptic processes. EAAT2 function finely regulated by endocytic events, recycling to the plasma membrane degradation. Noteworthy, deficits have been associated with neuronal excitotoxicity neurodegeneration. In this study, we show that trafficking impaired leucine-rich repeat kinase (LRRK2) pathogenic variant G2019S, a common cause late-onset...
Background: Parkinson’s disease is a progressive neurodegenerative disorder mainly distinguished by sporadic etiology, although genetic component also well established. Variants in the LRRK2 gene are associated with both familiar and disease. We have previously shown that PAK6 14-3-3γ protein interact regulate activity of LRRK2. Objective: The aim this study to quantify plasma as reliable biomarkers for diagnosis LRRK2-linked Methods: After an initial quantification expression means Western...
Abstract Astrocytes participate in the clearance of obsolete or unwanted neuronal synapses. However, molecular machinery recruited for recognition synapses is not fully clarified, especially pathological conditions. Here, we investigated phagocytic process through individual gene silencing using a druggable library. Our study demonstrates that astrocyte-mediated synapse engulfment regulated by Atypical chemokine receptor 3 (Ackr3). Mechanistically, have shown Ackr3 recognizes...
Human neuronal loss occurs through different cellular mechanisms, mainly studied in vitro. Here, we characterized death B. schlosseri, a marine colonial tunicate that shares substantial genomic homology with mammals and has life history which controlled neurodegeneration happens simultaneously the brains of adult zooids during cyclical phase named takeover. Using an ultrastructural transcriptomic approach, described forms before takeover while comparing their buds where are refining...
Mutations in SPG11, encoding spatacsin, constitute the major cause of autosomal recessive Hereditary Spastic Paraplegia (HSP) with thinning corpus callosum. Previous studies showed that spatacsin orchestrates cellular traffic events through formation a coat-like complex and its loss function results lysosomal axonal transport impairments. However, upstream mechanisms regulate trafficking are unknown. Here, using proteomics CRISPR/Cas9-mediated tagging endogenous we identified subset 14-3-3...
Abstract BackgroundParkinson’s disease (PD) is a neurodegenerative, progressive without cure. To prevent PD onset or at least limit neurodegeneration, better understanding of the underlying cellular and molecular mechanisms crucial. Mutations in leucine rich repeat kinase 2 ( LRRK2 ) gene represent one most common causes familial PD. In addition, variants are risk factors for sporadic PD, making an attractive therapeutic target. M utations has been linked to impaired alpha-synuclein (α-syn)...
2. Abstract Background Parkinson’s disease is a progressive neurodegenerative disorder mainly distinguished by sporadic aetiology, although genetic component also well established. Variants in the LRRK2 gene are associated with both familiar and disease. We have previously shown that PAK6 14-3-3γ protein interact regulate activity of LRRK2. Objectives The aim this study to quantify plasma as reliable biomarker strategy for diagnosis LRRK2-linked Methods After an initial quantification...
Abstract Mutations in SPG11 , encoding spatacsin, constitute the major cause of autosomal recessive Hereditary Spastic Paraplegia (HSP) with thinning corpus callosum. Previous studies showed that spatacsin orchestrates cellular traffic events through formation a coat-like complex and its loss function results lysosomal axonal transport impairments. However, upstream mechanisms regulate trafficking are unknown. Here, using proteomics CRISPR/Cas9-mediated tagging endogenous we identified...
Abstract The Excitatory Amino Acid Transporter 2 (EAAT2) accounts for 80 % of brain glutamate clearance and is mainly expressed in astrocytic perisynaptic processes. EAAT2 function finely regulated by endocytic events, recycling to the plasma membrane degradation. Noteworthy, deficits have been associated with neuronal excitotoxicity neurodegeneration. In this study, we show that trafficking impaired leucine-rich repeat kinase (LRRK2) pathogenic variant G2019S, a common cause late-onset...
Abstract Parkinson’s disease (PD) is a progressive neurodegenerative disorder mainly distinguished by sporadic aetiology, although genetic component also well established. Variants in the LRRK2 gene are associated with both familiar and forms of disease. We have previously shown that PAK6 14-3-3γ interact regulate activity LRRK2. In this study, we quantify total amount post-mortem tissue plasma samples to explore their potential as novel biomarkers for PD. The significantly different PD...
Abstract Microglia, the central nervous system resident immune cells, are now recognized to critically impact homeostasis maintenance and contribute outcomes of various pathological conditions including Parkinson’s disease (PD). Microglia heterogenous, with a variety states recently identified in aging neurodegenerative models, ‘disease-associated microglia’ (DAM) which present selective enrichment CLEC7A encoding or DECTIN1 protein, ‘dark (DM) displaying markers cellular stress at...