A5049485412- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Biotin and Related Studies
- Neuroscience and Neuropharmacology Research
- 14-3-3 protein interactions
- Cellular transport and secretion
- Signaling Pathways in Disease
- Microtubule and mitosis dynamics
- Cholesterol and Lipid Metabolism
- Diet, Metabolism, and Disease
- Fungal and yeast genetics research
- Biochemical Analysis and Sensing Techniques
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Nuclear Structure and Function
- Biochemical effects in animals
- Tryptophan and brain disorders
- Peroxisome Proliferator-Activated Receptors
- Cannabis and Cannabinoid Research
- Genetics and Neurodevelopmental Disorders
- Adenosine and Purinergic Signaling
- Lysosomal Storage Disorders Research
- Nuclear Receptors and Signaling
- Retinoids in leukemia and cellular processes
- Liver Disease Diagnosis and Treatment
University of Padua
2020-2024
IRCCS Humanitas Research Hospital
2023-2024
National Research Council
2023-2024
University of Naples Federico II
2017-2020
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2019
Mutations in LRRK2 cause familial Parkinson’s disease and common variants increase risk. kinase activity cellular localization are tightly regulated by phosphorylation of key residues, primarily Ser1292 Ser935, which impacts downstream its substrates, among Rab10. A comprehensive characterization brain as a function age mutations is missing. Here, we monitored Ser935 midbrain, striatum, cortex 1, 6, 12 months-old mice carrying G2019S R1441C or murine bacterial artificial chromosome...
In the central nervous system, cholesterol is critical to maintain membrane plasticity, cellular function, and synaptic integrity. recent years, much attention was focused on role of in brain since a breakdown metabolism has been associated with different diseases. Brain‐derived neurotrophic factor (BDNF) previously reported elicit biosynthesis promote accumulation presynaptic proteins cholesterol‐rich lipid rafts, but no data are available its ability modulate physiological mechanisms...
Abstract Inflammation and oxidative stress play an important role in the pathogenesis of depressive disorders nuclear erythroid related factor 2 (Nrf2), a regulator RedOx homeostasis inflammation, is promising target for depression prevention/treatment. As fish oil (FO) conjugated linoleic acid (CLA) are known Nrf2 inducers, their protective ability comparatively evaluated murine model (MRL/MpJ‐Fas lpr ). Oxidative stress, fatty acids content, critical factors reflecting brain...
Objectives: A strong rise of the fructose content in human diet occurred last decade, as corn syrup is widely used a sweetener for beverages and processed food. Since young people make widespread consumption added sugars, we evaluated effects two weeks fructose-rich on brain redox homeostasis, autophagy synaptic plasticity cortex adults rats, order to highlight early risks which exposed.Methods Results: Short-term feeding was associated with an imbalance lower amount Nuclear factor...
Parkinson’s disease (PD) is a multisystemic disorder that manifests through motor and non-motor symptoms. Motor dysfunction the most debilitating it caused by degeneration of dopamine-producing neurons in substantia nigra pars compacta (SNpc). A body evidence indicates synapse demise precedes years neuronal death. Still, early synaptic dysfunctions PD are poorly deciphered.Here we combined literature metanalysis, proteomics phosphoproteomics with biochemical, imaging electrophysiological...
Background: Parkinson’s disease is a progressive neurodegenerative disorder mainly distinguished by sporadic etiology, although genetic component also well established. Variants in the LRRK2 gene are associated with both familiar and disease. We have previously shown that PAK6 14-3-3γ protein interact regulate activity of LRRK2. Objective: The aim this study to quantify plasma as reliable biomarkers for diagnosis LRRK2-linked Methods: After an initial quantification expression means Western...
P21 activated kinase 6 (PAK6) is a serine-threonine with physiological expression enriched in the brain and overexpressed number of human tumors. While role PAK6 cancer cells has been extensively investigated, function context poorly understood. Our previous work uncovered link between Parkinson's disease (PD)-associated LRRK2, controlling LRRK2 activity subcellular localization via phosphorylation 14-3-3 proteins. Here, to gain more insights into function, we performed protein-protein...
Scope Cholesterol homeostasis is crucial for brain functioning. Unhealthy nutrition can influence cerebral physiology, but the effect of western diets on cholesterol homeostasis, particularly at middle age, unknown. Given link between alteration and beta amyloid production, aim to evaluate whether a diet rich in fat fructose affects protein network implicated synthesis shuttling glial cells neurons, as well markers metabolism. Methods results Middle aged rats are fed high fat–high (HFF) or...
Mutations in leucine-rich repeat kinase 2 (LRRK2) are a common cause of inherited and sporadic Parkinson’s disease (PD) previous work suggests that dephosphorylation LRRK2 at cluster heterologous phosphosites is associated to disease. We have previously reported subunits the PP1 PP2A classes phosphatases as well PAK6 regulators dephosphorylation. therefore hypothesized may functional link with LRRK2’s phosphatases. To investigate this, we used PhosTag gel electrophoresis purified proteins...
Pathological mutations in leucine-rich repeat kinase 2 (LRRK2) gene are the major genetic cause of Parkinson's disease (PD). Multiple lines evidence link LRRK2 to control vesicle dynamics through phosphorylation a subset RAB proteins. However, molecular mechanisms underlying these processes not fully elucidated. We have previously demonstrated that increases exocyst complex assembly by Sec8 interaction, one eight members complex, and over-expression mitigates pathological effect PC12 cells....
Abstract Parkinson’s disease (PD) is a multisystemic disorder that manifests through motor and non-motor symptoms. Motor dysfunction the most debilitating it caused by degeneration of dopamine-producing neurons in substantia nigra pars compacta (SNpc). A body evidence indicates synapse demise precedes years neuronal death. Still, early synaptic dysfunctions PD are poorly deciphered. Here we combined literature metanalysis, proteomics phosphoproteomics with biochemical, imaging...
Parkinson’s disease (PD) is a multisystemic disorder that manifests through motor and non-motor symptoms. Motor dysfunction the most debilitating it caused by degeneration of dopamine-producing neurons in substantia nigra pars compacta (SNpc). A body evidence indicates synapse demise precedes years neuronal death. Still, early synaptic dysfunctions PD are poorly deciphered.Here we combined literature metanalysis, proteomics phosphoproteomics with biochemical, imaging electrophysiological...
P21 activated kinase 6 (PAK6) is a serine-threonine with physiological expression enriched in the brain and overexpressed number of human tumors. While role PAK6 cancer cells has been extensively investigated, function context poorly understood. Our previous work uncovered link between Parkinson's disease (PD)-associated LRRK2, controlling LRRK2 activity subcellular localization via phosphorylation 14-3-3 proteins. Here, to gain more insights into function, we performed protein-protein...
<title>Abstract</title> P21 activated kinase 6 (PAK6) is a serine-threonine with physiological expression enriched in the brain and overexpressed number of human tumors. While role PAK6 cancer cells has been extensively investigated, function context poorly understood. Our previous work uncovered link between Parkinson’s disease (PD)-associated LRRK2, controlling LRRK2 activity subcellular localization <italic>via</italic> phosphorylation 14-3-3 proteins. Here, to gain more insights into...
Autophagy is a highly conserved homeostatic process essential for the bulk degradation of cytoplasmic components and aggregated proteins. Multiple evidence indicates that impairment (macro)autophagy leads to neurodegeneration, such as Parkinson disease (PD). Our previous work showed p21 activated kinase 6 (PAK6) interacts with PD-associated leucine-rich repeat (LRRK2) promote neurite outgrowth in mouse striatum; still function PAK6 brain largely unknown. Here, we found downregulation...
2. Abstract Background Parkinson’s disease is a progressive neurodegenerative disorder mainly distinguished by sporadic aetiology, although genetic component also well established. Variants in the LRRK2 gene are associated with both familiar and disease. We have previously shown that PAK6 14-3-3γ protein interact regulate activity of LRRK2. Objectives The aim this study to quantify plasma as reliable biomarker strategy for diagnosis LRRK2-linked Methods After an initial quantification...
Abstract Parkinson’s disease (PD) is a progressive neurodegenerative disorder mainly distinguished by sporadic aetiology, although genetic component also well established. Variants in the LRRK2 gene are associated with both familiar and forms of disease. We have previously shown that PAK6 14-3-3γ interact regulate activity LRRK2. In this study, we quantify total amount post-mortem tissue plasma samples to explore their potential as novel biomarkers for PD. The significantly different PD...