A5059379203- Pregnancy-related medical research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Sperm and Testicular Function
- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Injury Epidemiology and Prevention
- Alzheimer's disease research and treatments
- RNA regulation and disease
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive System and Pregnancy
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Reproductive Biology and Fertility
- Uterine Myomas and Treatments
- Genetics and Neurodevelopmental Disorders
- Endometriosis Research and Treatment
- Extracellular vesicles in disease
- Amyotrophic Lateral Sclerosis Research
- Reproductive biology and impacts on aquatic species
- Neurological diseases and metabolism
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
University of Naples Federico II
2014-2024
Institute of Protein Biochemistry
2022
National Research Council
2022
Institute of Genetics and Biophysics
2011-2013
Ceinge Biotecnologie Avanzate (Italy)
2004
Stazione Zoologica Anton Dohrn
2003
Abstract Endometriosis is a common benign pathology, characterised by the presence of endometrial tissue outside cavity with prevalence 10–15% in reproductive‐aged women. The pathogenesis not completely understood, and several theories have been proposed to explain aetiology. Our group has recently described ectopic endometrium consistent number human female foetuses analysed autopsy, reinforcing hypothesis that endometriosis may be generated defects during organogenesis reproductive trait....
Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved various aspects of the pathogenesis. However, these suffer from several limits drawbacks and, date, information about rare, even though relevant, RNA species such as long small non-coding RNAs, is completely missing. Indeed, none published works has still described whole transcriptional landscape syndrome. Although recent advances high-throughput sequencing revealed complexity...
In the central nervous system, cholesterol is critical to maintain membrane plasticity, cellular function, and synaptic integrity. recent years, much attention was focused on role of in brain since a breakdown metabolism has been associated with different diseases. Brain‐derived neurotrophic factor (BDNF) previously reported elicit biosynthesis promote accumulation presynaptic proteins cholesterol‐rich lipid rafts, but no data are available its ability modulate physiological mechanisms...
The CD33 gene encodes for a member of the sialic-acid-binding immunoglobulin-type lectin (Siglec) family, and is one top-ranked Alzheimer’s disease (AD) risk genes identified by genome-wide association studies (GWAS). Many polymorphisms are associated with an increased AD, but function potential mechanism many single-nucleotide (SNPs) in promoting AD have yet to be elucidated. We recently SNP rs2455069-A>G (R69G) familial form dementia. Here, we demonstrate between G allele rs2455069 variant...
Background Malignant mesothelioma (MM) is a rare, highly aggressive tumor, associated to asbestos exposure. To date no chemotherapy regimen for MM has proven be definitively curative, and new therapies treatment need developed. We have previously shown in vivo that piroxicam/cisplatin combined MM, specifically acts on cell cycle regulation triggering apoptosis, with survival increase. Methodology/Principal Findings analyzed, at molecular level, the apoptotic increase caused by lines. By...
Abstract We examined the spatial expression of relaxin ‐3 gene in developing zebrafish brain, one vertebrate model systems which this has been identified. Until pharyngula stage, is expressed diffusely where, starting at about 40 hpf, transcripts appear restricted a midbrain cell cluster periaqueductal gray. Later, 72 are still evident that and distributed larger number; also posteriorly, smaller group that, as we report for first time, could be homologous to mammalian nucleus incertus. The...
Dishevelled‐associated activator of morphogenesis 1 (DAAM1) is a formin‐family protein involved in nucleation unbranched actin filaments and cytoskeletal organization through Wnt‐Dishevelled PCP pathway, which participates essential biological processes, such as cell polarity, movement, adhesion during organogenesis. While its role has been investigated development somatic cells, potential association with the germinal compartment reproduction still unexplored. In this work, we assessed...
Relaxin-3 (Rln3) is thought to function as a neurotransmitter mainly produced in the mammalian nucleus incertus and involved different neural processes; among them, stress response food intake. Here, we report expression pattern of duplicated zebrafish rln3b gene compare it previously analyszd spatial rln3a gene. Both genes, during embryogenesis adult fish, are active show relevant differences their patterns. diffusely expressed brain until pharyngula period, when, at 48 h postfertilization...
Brain-derived neurotrophic factor (BDNF) is a neurotrophin family member that highly expressed and widely distributed in the brain. BDNF critical for neural survival plasticity both during development adulthood, dysfunction its signaling may contribute to number of neurodegenerative disorders. Deep understanding BDNF-activated molecular cascade thus help find new biomarkers therapeutic targets. One interesting direction related early phase BDNF-dependent gene expression regulation, which...
Prothymosin α (PTMA) is a highly acidic, intrinsically disordered protein, which widely expressed and conserved throughout evolution; its uncommon features are reflected by involvement in variety of processes, including chromatin remodelling, transcriptional regulation, cell proliferation death, immunity. PTMA has also been implicated spermatogenesis: during vertebrate germ progression the testis protein meiotic post-meiotic stages, it associated with acrosome system differentiating...
Prothymosin alpha (PTMA) is a highly acidic small polypeptide, that is, widely distributed and conserved among mammals. Its possible involvement in male gametogenesis has been mentioned but not clarified yet; particular, it suggested that, non-mammalian vertebrates, could play role during GC meiosis differentiation. In the present work we investigated association between PTMA meiotic post-meiotic phases of mammalian spermatogenesis. Three different time points postnatal development rat...
The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis vertebrates. Reasoning that zebrafish genes uncx4.1 and uncx are orthologs mouse , we studied their genomic environment developmental expression. Evolutionary analyses indicate the as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mount situ mRNA hybridization transcripts embryos reveals novel expression domains, confirms those previously known, suggests...
Maternal stress during pregnancy adversely affects developmental fetal programming. Glucocorticoid excess is one of those conditions that underlie the prenatal and can lead to many pathological disorders later in life. Beyond obvious use mammalian model organisms uncover different mechanisms at basis effects, zebrafish represents a complementary fruitful for this research field. Here we demonstrated application an experimental paradigm, which simulates by exposing embryos cortisol excess,...
Abstract We show that ptma , a single copy gene found in all organisms investigated so far, is duplicated zebrafish. The two genes, and b, are individually controlled as indicated by their different expression patterns during embryonic development. Only the b transcript observed at 4 8 hpf of development cells, whereas both genes expressed later stages revealed situ hybridization studies. In most cases, same territories, but only was trigeminal ganglion endodermal pouches. eye, 72 hpf,...
Insulin-like peptide 3 (INSL3) is a biomarker for Leydig cells in the testes of vertebrates, and it principally involved spermatogenesis through specific binding with RXFP2 receptor. This study reports insl3 gene transcript Insl3 prepropeptide expression both non-reproductive reproductive tissues Danio rerio. An immunohistochemistry analysis shows that hormone present at low level germ all stages rerio testis differentiation. Considering transcribed cells, our results highlight an autocrine...
Frontotemporal dementia (FTD) is the second most common cause of early-onset after Alzheimer disease (AD). Efforts in field mainly focus on familial forms (fFTDs), while studies genetic etiology sporadic FTD (sFTD) have been less common. In current work, we analyzed 4,685 sFTD cases and 15,308 controls looking for determinants sFTD. We found a cluster variants at MAPT (rs199443; p = 2.5 × 10-12, OR 1.27) APOE (rs6857; 1.31 loci candidate locus chromosome 3 (rs1009966; 2.41 10-8, 1.16)...
We report the gene characterization, cDNA cloning and temporal spatial expression pattern of relaxin receptor rxfp1 in zebrafish Danio rerio. The has same syntenic genomic organization, a similar exon-intron structure to homologue human gene. Furthermore, deduced Rxfp1 protein sequence shows high degree amino acid similarity when compared with conservation all identity necessary for binding relaxin. Our results show that is active either during embryogenesis or adult organism, showing wide...
In mammals, the RXFP3 is cognate receptor of relaxin-3 peptide (RLN3). teleosts, many different orthologue genes for are present. particular, two paralogue genes, rxfp3-2a and rxfp3-2b, likely encode receptors Rln3a peptide. The transcription these rxfp3 differentially regulated early during zebrafish embryogenesis. Indeed, reverse transcription-polymerase chain reaction analyses show that rxfp3-2b transcript always present embryo development, while detectable only at larval stage. By in...