A5007254109- Liver Disease Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Parkinson's Disease Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Metabolism, Diabetes, and Cancer
- Liver Diseases and Immunity
- Pancreatic function and diabetes
- Magnolia and Illicium research
- Muscle Physiology and Disorders
- Hepatitis C virus research
- Liver Disease and Transplantation
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Neurological disorders and treatments
- RNA Interference and Gene Delivery
- Biochemical effects in animals
- Cancer-related Molecular Pathways
- Adipose Tissue and Metabolism
- Pharmacological Effects of Natural Compounds
- Diet, Metabolism, and Disease
- Pancreatic and Hepatic Oncology Research
- Neurotransmitter Receptor Influence on Behavior
- Coenzyme Q10 studies and effects
Saiseikai Suita Hospital
2016-2024
Hokuriku University
2007-2019
Kyoto first Red Cross hospital
2014
Nara City Hospital
2014
Kyoto Prefectural University of Medicine
1999-2012
Otsuka (Japan)
1998-2006
Otsuka Pharmaceutical (Spain)
2005
Tokushima Bunri University
2001-2004
Institute for Neurodegenerative Disorders
2001
Tohoku University
1998
Unlike glucose transport, where translocation of the insulin-responsive transporter (GLUT4) from an intracellular compartment to plasma membrane is principal mechanism underlying insulin stimulation, no consensus exists presently for by which activates Na+/K(+)-ATPase. We have investigated (i) subunit isoforms expressed and (ii) effect on subcellular distribution alpha beta Na+/K(+)-ATPase in membranes (PM) internal (IM) rat skeletal muscle. Western blot analysis, using isoform-specific...
Background and Purpose —Glial cell line–derived neurotrophic factor (GDNF) plays important roles in the survival recovery of some mature neurons under pathological conditions. However, effect GDNF ameliorating ischemic brain injury has not been well documented. Therefore, we investigated a possible on changes infarct size, edema, DNA fragmentation, immunoreactivities for caspases after permanent middle cerebral artery occlusion (MCAO) rats. Methods —For estimation injury, calculated size MCA...
The genetic factors affecting the natural history of nonalcoholic fatty liver disease (NAFLD), including development steatohepatitis (NASH) and NASH-derived hepatocellular carcinoma (NASH-HCC), are still unknown. In current study, we sought to identify related NAFLD, NASH, NASH-HCC, establish risk-estimation models for them. For these purposes, 936 histologically proven NAFLD patients were recruited, genome-wide association (GWA) studies conducted 902, 476 NASH 58 NASH-HCC patients, against...
PNPLA3 rs738409 has been associated with an increased risk of liver-related events in patients non-alcoholic fatty liver disease (NAFLD). In this study, we investigated the epidemiology NAFLD and impact on prognosis Japan.A longitudinal multicentre cohort JAGUAR includes 1550 biopsy-proven Japan. We performed genetic testing evaluated outcomes from cohort. Liver-related were defined as hepatocellular carcinoma (HCC) decompensated cirrhosis events.During follow-up (median [range], 7.1...
L6 myoblasts spontaneously undergo differentiation and cell fusion into myotubes. These cells express both GLUT1 GLUT4 glucose transporters, but their expression varies during myogenesis. We now report that the subcellular distribution protein processing by glycosylation of transporter isoforms also change Crude plasma membrane light microsome fractions were isolated from either or myotubes characterized presence two functional proteins, Na+/K(+)-ATPase dihydropyridine receptor (DHPR)....
Background: Factors associated with response to lenvatinib have not been clarified in patients hepatocellular carcinoma (HCC). Patients and Methods: This study retrospectively analyzed 50 treated as first-line therapy between March 2018 2019. were divided into two groups by the Modified Response Evaluation Criteria Solid Tumours (mRECIST) (responders non-responders, whose best overall responses complete (CR)/partial (PR) stable (SD)/progressive disease (PD), respectively). assessed,...
Abstract Background/Aims Statins, which are inhibitors of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase and inhibit endogenous cholesterol synthesis, possess pleiotropic activities, such as anti‐inflammatory, anti‐oxidative antifibrotic effects. Here, we investigated whether statins ameliorate steatohepatitis using a high‐fat high‐cholesterol ( HFHC ) diet‐induced rat model. Methods Eight‐week‐old male Sprague–Dawley rats were fed control chow or diet. Half the diet‐fed orally administered...
Abstract Aim We aimed to develop a novel noninvasive test using an artificial intelligence (AI)/neural network (NN) system (named nonalcoholic steatohepatitis [NASH]‐Scope) screen fatty liver disease (NAFLD) and NASH. Methods enrolled 324 74 patients histologically diagnosed with NAFLD for training validation studies, respectively. Two independent pathologists study. Additionally, 48 subjects who underwent medical health checkup did not show ultrasonographically had normal serum...
To examine a possible protective effect of exogenous glial cell line-derived neurotrophic factor (GDNF) gene expression against ischemic brain injury, replication-defective adenoviral vector containing GDNF (Ad-GDNF) was directly injected into the cerebral cortex at 1 day before 90 minutes transient middle artery occlusion (MCAO) in rats. 2,3,5-Triphenyltetrazolium chloride staining showed that infarct volume Ad-GDNF-injected group 24 hours after MCAO significantly smaller than vehicle- or...
Recent studies have revealed that copper is an important cofactor for several angiogenic agents. We examined the antiangiogenic effect against hepatocellular carcinoma (HCC) of chelator trientine, especially focusing on relationship between and interleukin-8 (IL-8), a potent factor produced by hepatoma cells. HuH-7 cells were transplanted into nude mice growth xenograft tumors was compared to without administration trientine. Using resected tumor, microvessel density, apoptotic potential...
Several reports have noted epidemiological differences in the prevalence or prognostic significance of p53 mutants with arginine (R) proline (P) at codon 72 polymorphism (R72/P72) certain cancer types, but biological these variants is unclear. The ability to interact and inactivate homolog p73 was recently reported depend on conformational state protein residue 72. Since conformation may influence their transdominantly inhibit wild-type p53, we tested whether there a correlation between...
Non-alcoholic fatty liver disease (NAFLD) can progress to non-alcoholic (NAFL) or steatohepatitis (NASH). We investigated the association among serum type IV collagen level, histology, and other fibrosis markers in NAFLD progression.We evaluated 184 patients diagnosed with following biopsy, including 89 males 95 females an average age of 52.6 62.6 years, respectively. was classified as NAFL NASH using Matteoni's classification, grade stage were assessed Brunt's classification. Serum measured...
This study aimed to determine the safety and efficacy of atezolizumab + bevacizumab therapy in hepatocellular carcinoma patients receiving anti-platelet agents or anticoagulants.
The effects of insulin and IGF‐I on the cell surface quantities GLUT1, GLUT3 GLUT4 glucose transporters in L6 myotubes were determined with exofacial bis‐mannose phololabel (ATB‐BMPA). In basal cells, an equal molar quantity each transporter isoform was found at surface. Insulin stimulated translocation all three isoforms to plasma membrane fraction from light microsome fraction, resulting a similar isoforms, augmented by increase as assessed ATB‐BMPA.
Molecular studies have identified a family of synaptic vesicle-associated membrane proteins (VAMPs, also known as synaptobrevins) which been implicated in vesicle docking and/or fusion with plasma proteins. Here we demonstrate the expression two members this family, VAMP-2/synaptobrevin II and cellubrevin, skeletal muscle, tissue both constitutive regulated traffic. The 18 kDa VAMP-2 polypeptide was detected purified fractions from adult muscle L6 myotubes culture, demonstrating that...