A5082432453- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- Renal and related cancers
- Chemical Synthesis and Analysis
- Microfluidic and Bio-sensing Technologies
- Tissue Engineering and Regenerative Medicine
- Advanced oxidation water treatment
- Advanced battery technologies research
- Pluripotent Stem Cells Research
- Amino Acid Enzymes and Metabolism
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Fungal and yeast genetics research
- Environmental remediation with nanomaterials
- Music Technology and Sound Studies
- Electrochemical Analysis and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Modular Robots and Swarm Intelligence
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Pancreatic function and diabetes
- Enzyme Structure and Function
Guangzhou Institutes of Biomedicine and Health
2021-2024
Chinese Academy of Sciences
2023-2024
Guangzhou Medical University
2021-2023
East China University of Science and Technology
2016-2022
University of Georgia
2022
Chalmers University of Technology
2020
Technische Universität Berlin
2020
University of Science and Technology
2020
Acoustic tweezers, capable of complicated manipulation bioparticles by acoustic radiation forces using a noninvasive and noncontact approach, are an innovative technology for patterning assemble organoids. Hence, tweezers exhibit considerable potential forming programmable organoids with specific spatial structures. Furthermore, heterogeneous assembloids complex arrangement patterns can be built through sequential assembling culturing to explore polarized tissue development or disease...
Abstract Oxygen limitation is regarded as a useful strategy to improve enzyme production by mycelial fungus like Aspergillus niger . However, the intracellular metabolic response of A. oxygen still obscure. To address this, metabolism was studied using multi-omics integrated analysis based on latest GEMs (genome-scale model), including metabolomics, fluxomics and transcriptomics. Upon sharp reduction supply, shifted higher redox level status, well lower energy down-regulation genes for fatty...
Abstract Background Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis the filamentous fungal factory Aspergillus niger indicated that low availability NADPH might be a limiting factor for GlaA overproduction. Results We thus employed Design-Build-Test-Learn cycle metabolic engineering to identify prioritize...
The use of differentiating human induced pluripotent stem cells (hiPSCs) in mini-tissue organoids provides an invaluable resource for regenerative medicine applications, particularly the field disease modeling. However, most studies using a kidney organoid model, focused solely on transcriptomics and did not explore mechanisms regulating related to metabolic effects maturational phenotype. Here, we applied metabolomics coupled with investigate dynamics function during differentiation. Not...
Astrocytes are the most abundant and widespread glial cells in central nervous system. The heterogeneity of astrocytes plays an essential role spinal cord injury (SCI) repair. Decellularised matrix (DSCM) is advantageous for repairing SCI, but little known regarding exact mechanisms niche alterations. Here, we investigated DSCM regulatory mechanism neuro-glial-vascular unit using single-cell RNA sequencing. Our single cell sequencing, molecular biochemical experiments validated that...
Abstract Omics approaches have been applied to understand the boosted productivity of natural products by industrial high‐producing microorganisms. Here, with updated genome sequence and transcriptomic profiles derived from high‐throughput sequencing, we exploited comparative omics analysis further enhance biosynthesis erythromycin in an overproducer, Saccharopolyspora erythraea HL3168 E3. By comparing E3 wild type NRRL23338, identified fragment deletions inside 56 coding sequences 255...
Abstract Background Glucoamylase is one of the most industrially applied enzymes, produced by Aspergillus species, like niger . Compared to traditional ways process optimization, metabolic engineering strategies improve glucoamylase production are relatively scarce. Results In previous study combined multi-omics integrative analysis and amino acid supplementation experiment, we predicted four acids (alanine, glutamate, glycine aspartate) as limited precursors for in A. To further verify...
In the phase of oxygen limitation during Aspergillus niger fermentation, cell growth decreased, while yield glucoamylase increased continuously and significantly. Explanation on changes transcriptome profile this process may improve our understanding mechanisms adaption enzyme production in A. niger. The transcriptomic data from 4 time points a strain were analyzed. Hierarchical clustering all samples showed that strongest transcriptional response occurred between early middle stage...
Formation of chlorate (ClO3-) and perchlorate (ClO4-) as by-products in electrooxidation process has raised concern. In the present study, formation ClO3- ClO4- presence 1.0 mM Cl- on boron doped diamond (BDD) Magneli phase titanium suboxide (Ti4O7) anodes were evaluated. The was transformed to (temporal maximum 276.2 μM) first 0.5 h BDD with a constant current density 10 mA cm2, while approximately 1000 μM formed after 4.0 h. Ti4O7 anode slower, reaching temporary 350.6 h, also slower...
Acoustic Tweezer for Heterogenous Assembloids Patterning Heterogeneous assembloids with predesigned patterns can be constructed sequentially to explore polarized tissue development or disease metastasis in multi-organs. In article 2200288, Hairong Zheng, Xiao Zhang, Teng Ma, and co-workers present a multifunctional acoustic tweezer system that manipulates single organoid construct non-invasively free-label—this pioneering study demonstrates the possibility of bulk-wave application engineering.
Abstract Background: Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis the filamentous fungal factory Aspergillus niger indicated that low availability NADPH might be a limiting factor for GlaA overproduction. Results: We thus employed Design-Build-Test-Learn cycle metabolic engineering to identify prioritize...
Abstract Background: Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis the filamentous fungal factory Aspergillus niger indicated that low availability NADPH might be a limiting factor for GlaA overproduction. Results: We thus employed Design-Build-Test-Learn cycle metabolic engineering to identify prioritize...
Abstract Formation of chlorate (ClO 3 − ) and perchlorate 4 as by-products in electrooxidation process has raised concern. In the present study, formation ClO presence 1.0 mM Cl on boron doped diamond (BDD) Magneli phase titanium suboxide (Ti O 7 anodes were evaluated. The was transformed to (temporal maximum 276.2 µM) first 0.5 h BDD with a constant current density 10 mA cm 2 , while approximately 1000 µM formed after 4.0 h. Ti anode slower, reaching temporary 350.6 h, also slower anode,...
Omics approaches have been applied to understand the boosted productivity of natural products by industrial high-producing microorganisms. Here, with updated genome sequence and transcriptomic profiles derived from high-throughput sequencing, we exploited comparative omics analysis further enhance biosynthesis erythromycin in an overproducer, Saccharopolyspora erythraea HL3168 E3. By comparing E3 wild type NRRL23338, identified fragment deletions inside 56 coding sequences 255 single...