A5091781696- Malaria Research and Control
- Drug Transport and Resistance Mechanisms
- Mosquito-borne diseases and control
- Toxoplasma gondii Research Studies
- HIV/AIDS drug development and treatment
- Autophagy in Disease and Therapy
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Drug-Induced Hepatotoxicity and Protection
Georgetown University
2017-2020
Toxoplasma gondii is an apicomplexan parasite with the ability to use foodborne, zoonotic, and congenital routes of transmission that causes severe disease in immunocompromised patients. The parasites harbor a lysosome-like organelle, termed "Vacuolar Compartment/Plant-Like Vacuole" (VAC/PLV), which plays important role maintaining lytic cycle virulence T. gondii. VAC supplies proteolytic enzymes contribute maturation invasion effectors digest autophagosomes endocytosed host proteins....
The Plasmodium falciparum malarial parasite genome appears to encode one and only phosphatidylinositol 3'-kinase (PI3K), sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as possible druggable target potentially key of artemisinin-based antimalarials. In this study, we optimize gene for heterologous expression in yeast, purify protein homogeneity, use recently validated quantitative assay 3'-phosphate production from ( Hassett...
Patterns of multiple amino acid substitutions in the Plasmodium falciparum chloroquine resistance transporter (PfCRT, UniProtKB Q8IBZ9) have previously been shown to mediate P. malarial parasites. Recent reports suggest that novel mutations PfCRT may piperaquine (PPQ), which is used extensively as a partner drug one prominent artemisinin combination therapy. How these isoforms might PPQ (PPQR) not known. Using codon optimization and other perfected methods for analysis yeast, we expressed...
Chloroquine (CQ) resistance (CQR) in Plasmodium falciparum malaria is widespread and has limited the use of CQ many regions globe. Malaria caused by related human parasite P. vivax as been treated with extensively falciparum, suggesting that parasites have selected profoundly parasites. Indeed, a growing number clinical reports presented data increased CQR. Cytostatic (growth inhibitory) CQR for chloroquine transporter (PfCRT) mutations, it proposed mutations PvCRT orthologue may simliarly...
Abstract Toxoplasma gondii is an apicomplexan parasite with the ability to use foodborne, zoonotic, and congenital routes of transmission that causes severe disease in immunocompromised patients. The parasites harbor a lysosome-like digestive vacuole, termed “Vacuolar Compartment/Plant-Like Vacuole” (VAC/PLV), which plays important role maintaining lytic cycle virulence T. . VAC supplies proteolytic enzymes are required mature parasite’s invasion effectors digest autophagosomes endocytosed...