A5004692201- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Heme Oxygenase-1 and Carbon Monoxide
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Enzyme Structure and Function
- Porphyrin Metabolism and Disorders
- Autophagy in Disease and Therapy
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Trace Elements in Health
- Neonatal Health and Biochemistry
- Aquaculture disease management and microbiota
- Polyamine Metabolism and Applications
- Protein Structure and Dynamics
- HIV Research and Treatment
- Microbial infections and disease research
- Malaria Research and Control
- Calpain Protease Function and Regulation
- Enzyme function and inhibition
- HIV/AIDS drug development and treatment
- Biotin and Related Studies
- Vector-borne infectious diseases
- RNA Interference and Gene Delivery
Clemson University
2018-2024
University of Michigan–Ann Arbor
2011-2022
University of Glasgow
2022
Argonne National Laboratory
2022
Harding University Main Campus
2022
University of Southern Mississippi
2008-2015
The widely accepted models for the role of carboxysomes in carbon-concentrating mechanism autotrophic bacteria predict carboxysomal carbonic anhydrase to be a crucial component. enzyme is thought dehydrate abundant cytosolic bicarbonate and provide ribulose 1.5-bisphosphate carboxylase/oxygenase (RubisCO) sequestered within carboxysome with sufficiently high concentrations its substrate, CO(2), permit efficient fixation onto 1,5-bisphosphate. In this study, structure function purified from...
ABSTRACT The protozoan parasite Toxoplasma gondii resides within a nonfusogenic vacuole during intracellular replication. Although the limiting membrane of this provides protective barrier to acidification and degradation by lysosomal hydrolases, it also physically segregates from host cytosol. Accordingly, has been suggested that T. acquires material via channels or transporters. ability internalize macromolecules endocytosis replication not tested. Here, we show ingests cytosolic proteins...
The marine Synechococcus and Prochlorococcus are the numerically dominant cyanobacteria in ocean important global carbon fixation. They have evolved a CO2-concentrating-mechanism, of which central component is carboxysome, self-assembling proteinaceous organelle. Two types α β, encapsulating form IA IB d-ribulose-1,5-bisphosphate carboxylase/oxygenase, respectively, differ gene organization associated proteins. In contrast to β-carboxysome, assembly process α-carboxysome enigmatic. Moreover,...
Abstract Iron is essential to cells as a cofactor in enzymes of respiration and replication, however without correct storage, iron leads the formation dangerous oxygen radicals. In yeast plants, transported into membrane-bound vacuole by vacuolar transporter (VIT). This conserved apicomplexan family obligate intracellular parasites, including Toxoplasma gondii . Here, we assess role VIT storage T. By deleting VIT, find slight growth defect vitro, hypersensitivity, confirming its parasite...
The vacuolar proton pyrophosphatase (H(+) -PPase) of Toxoplasma gondii (TgVP1), a membrane pump, localizes to acidocalcisomes and novel lysosome-like compartment termed plant-like vacuole (PLV) or (VAC). We report the characterization T. null mutant for TgVP1 gene. Propagation these mutants decreased significantly because deficient attachment invasion host cells, which correlated with microneme secretion. Processing cathepsin L (CPL) in was only when parasites were incubated presence low...
Background The carboxysome is a bacterial microcompartment that consists of polyhedral protein shell filled with ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO), the enzyme catalyzes first step CO2 fixation via Calvin-Benson-Bassham cycle. Methodology/Principal Findings To analyze role RubisCO in biogenesis vivo we have created series Halothiobacillus neapolitanus mutants. We identified large subunit as an important determinant for its sequestration into α-carboxysomes and found...
Heme, an iron-containing organic ring, is essential for virtually all living organisms by serving as a prosthetic group in proteins that function diverse cellular activities ranging from diatomic gas transport and sensing, to mitochondrial respiration, detoxification. Cellular heme levels microbial pathogens can be composite of endogenous de novo synthesis or exogenous uptake intermediates. Intracellular pathogenic microbes switch routes supply when availability fluctuates their replicative...
Toxoplasma gondii is an apicomplexan parasite with the ability to use foodborne, zoonotic, and congenital routes of transmission that causes severe disease in immunocompromised patients. The parasites harbor a lysosome-like organelle, termed "Vacuolar Compartment/Plant-Like Vacuole" (VAC/PLV), which plays important role maintaining lytic cycle virulence T. gondii. VAC supplies proteolytic enzymes contribute maturation invasion effectors digest autophagosomes endocytosed host proteins....
<i>Toxoplasma gondii</i> is the model parasite of phylum Apicomplexa, which contains obligate intracellular parasites medical and veterinary importance. Apicomplexans invade host cells by a multistep process involving secretion adhesive microneme protein (MIC) complexes. The subtilisin protease TgSUB1 trims several MICs on surface to activate gliding motility invasion. Although previous study showed that expression secretory TgMIC5 suppresses activity, mechanism was unknown. Here, we solve...
ABSTRACT Mutations in the Plasmodium falciparum chloroquine resistance transporter ( Pf CRT) protein confer to antimalarial drug chloroquine. CRT localizes parasite digestive vacuole, site of action, where it mediates by transporting out vacuole. belongs a family proteins called family. are found throughout Apicomplexa, some protists, and plants. Despite importance resistance, little is known about evolution or native function proteins. The apicomplexan Toxoplasma gondii contains one...
Roughly one-third of the human population is chronically infected with intracellular single-celled parasite Toxoplasma gondii , but little known about how this organism persists inside people. Previous research suggested that a proteolytic enzyme, termed cathepsin protease L, important for persistence; however, it remained possible other associated enzymes could also be involved in long-term survival during infection. Here, we show two L play dispensable roles and are dependent on to reach...
Toxoplasma gondii is a widespread intracellular protozoan pathogen infecting virtually all warm-blooded animals. This parasite acquires host-derived resources to support its replication inside membrane-bound parasitophorous vacuole within infected host cells. Previous research has discovered that actively endocytoses proteins and transports them lysosome-equivalent structure for digestion. However, few molecular determinants required trafficking of material the were known. A recent study...
ABSTRACT Toxoplasma gondii is an apicomplexan parasite that the cause of toxoplasmosis, a potentially lethal disease for immunocompromised individuals. During in vivo infection, parasites encounter various growth environments, such as hypoxia. Therefore, metabolic enzymes must adapt to changes fulfill their nutritional requirements. can de novo biosynthesize some nutrients, heme. The heavily rely on own heme production intracellular survival. Notably, antepenultimate step within this pathway...
Toxoplasma gondii is a protozoan pathogen that widely affects the human population. The current antibiotics used for treating clinical toxoplasmosis are limited. In addition, they exhibit adverse side effects in certain groups of people. Therefore, discovery novel therapeutics imperative. first step antibiotic development to identify chemical compounds showing high efficacy inhibition parasite growth using throughput screening strategy. As an obligate intracellular pathogen, can only...
Abstract Iron is essential to living cells, acting as a cofactor in number of enzymes metabolism; however, iron requires proper storage or it can be dangerous the cell. In both yeast and plants, stored vacuole through action vacuolar transporter (VIT). This conserved apicomplexan family obligate intracellular parasites, including Toxoplasma gondii , pathogen medical veterinary importance. Here, we assess role VIT T. . We show that deletion causes slight growth defect vitro however leads...
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ABSTRACT Toxoplasma gondii is an apicomplexan parasite that the cause of toxoplasmosis, a potentially lethal disease for immunocompromised individuals. During in vivo infection, parasites encounter various growth environments, such as hypoxia. Therefore, metabolic enzymes must adapt to changes fulfill their nutritional requirements. can de novo biosynthesize some nutrients, heme. The heavily rely on own heme production intracellular survival. Notably, antepenultimate step within this pathway...
Abstract Iron is essential to living cells, acting as a cofactor in number of important enzymes metabolism; however the absence correct storage iron forms dangerous oxygen radicals. In both yeast and plants, stored membrane-bound vacuole through action vacuolar transporter (VIT). This conserved apicomplexan family obligate intracellular parasites, including Toxoplasma gondii , pathogen medical veterinary importance. Here, we assess role VIT, storage, T. gondii. We show that restricted...
Most commercial products cannot be used for clearance of