A5086870303- Toxoplasma gondii Research Studies
- Autophagy in Disease and Therapy
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Mosquito-borne diseases and control
- ATP Synthase and ATPases Research
- Escherichia coli research studies
- Vibrio bacteria research studies
- Fecal contamination and water quality
- Trace Elements in Health
- Vector-borne infectious diseases
- RNA regulation and disease
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Endoplasmic Reticulum Stress and Disease
- Herpesvirus Infections and Treatments
University of Georgia
2016-2023
Center for Biologics Evaluation and Research
2021
United States Food and Drug Administration
2021
University of Wisconsin–Madison
2012-2016
Vacuolar-proton ATPases (V-ATPases) are conserved complexes that couple the hydrolysis of ATP to pumping protons across membranes. V-ATPases known play diverse roles in cellular physiology. We studied Toxoplasma gondii V-ATPase complex and discovered a dual role pump protecting parasites against ionic stress maturation secretory proteins endosomal-like compartments. subunits localize plasma membrane acidic vesicles, characterization conditional mutants a1 subunit highlighted functionality at...
Abstract Calcium ions regulate a diversity of cellular functions in all eukaryotes. The cytosolic Ca 2+ concentration is tightly regulated at the physiological 50–100 n m . Toxoplasma gondii genome predicts presence several genes encoding potential channels, pumps, and transporters. Many these are weakly expressed likely due to their impact physiology cell. Endogenous tagging has been widely used localize proteins T. but low level expression many them makes visualization tags difficult...
Toxoplasma gondii is an intracellular pathogen of human and animals. T. pathogenesis associated with its lytic cycle, which involves invasion, replication, egress out the host cell, invasion a new one. must be able to tolerate abrupt changes in composition surrounding milieu as it progresses through cycle. We report characterization Zn 2+ transporter (TgZnT) that important for parasite growth. TgZnT restored tolerance yeast mutants were unable grow media high concentrations . propose plays...
Toxoplasma gondii is an apicomplexan parasite with the ability to use foodborne, zoonotic, and congenital routes of transmission that causes severe disease in immunocompromised patients. The parasites harbor a lysosome-like organelle, termed "Vacuolar Compartment/Plant-Like Vacuole" (VAC/PLV), which plays important role maintaining lytic cycle virulence T. gondii. VAC supplies proteolytic enzymes contribute maturation invasion effectors digest autophagosomes endocytosed host proteins....
Ca2+ is a universal intracellular signal that regulates many cellular functions. In Toxoplasma gondii, the controlled influx of extracellular and into cytosol initiates signaling cascade promotes pathogenic processes like tissue destruction dissemination. this work, we studied role proton transport in cytosolic homeostasis initiation signaling. We used T. gondii mutant V-H+ -ATPase, pump previously shown to protons medium, control pH membrane potential show gradients are important for...
A pen infection-transmission experiment was conducted to elucidate the role of pathogen strain and environmental contamination in transmission Escherichia coli O157:H7 (ECO157) cattle. Five steers were inoculated with a three-strain mixture ECO157 joined five susceptible each two experimental replicates. Faecal samples monitored for presence over 30 days. One did not spread. Transmission rates other strains estimated using generalized linear model developed based on modified...
The outer membrane of Salmonella enterica plays an important role in combating stress encountered the environment and hosts. transport insertion lipopolysaccharides (LPS) into involves lipopolysaccharide proteins (LptA-F) mutations genes encoding for these are often lethal or result atypical LPS that can alter tolerance bacteria. During studies heterogeneity bile salts tolerance, S. serovar Typhimurium E40 was segregated tolerant sensitive cells by screening growth TSB with 10% salts. An...
Microbial pathogens use proteases for their infections, such as digestion of proteins nutrients and activation virulence factors. As an obligate intracellular parasite, Toxoplasma gondii must invade host cells to establish its propagation. To facilitate invasion, the parasites secrete invasion effectors from microneme rhoptry, two unique organelles in apicomplexans. Previous work has shown that some micronemal experience a series proteolytic cleavages within parasite's secretion pathway...
ABSTRACT Microbial pathogens use proteases for their infections, such as digestion of proteins nutrients and activation virulence factors. As an obligate intracellular parasite, Toxoplasma gondii must invade host cells to establish its propagation. To facilitate invasion, the parasites secrete invasion effectors from microneme rhoptry, two unique organelles in apicomplexans. Previous work has shown that some micronemal experience a series proteolytic cleavages within parasite’s secretion...
Vacuolar-proton ATPases (V-H+-ATPases) are conserved complexes that couple the hydrolysis of ATP to pumping protons across membranes. V-H+-ATPases known play diverse roles in cellular physiology. We studied Toxoplasma gondii V-H+-ATPase complex and discovered a novel dual role pump protecting parasites against ionic stress maturation secretory proteins endosomal-like compartments. subunits localize plasma membrane acidic vesicles characterization conditional mutants a1 subunit highlighted...