A5101726102- Toxoplasma gondii Research Studies
- Lymphoma Diagnosis and Treatment
- Parasitic Infections and Diagnostics
- Cytomegalovirus and herpesvirus research
- Soft tissue tumor case studies
- Foot and Ankle Surgery
- Diabetes Management and Research
- Trypanosoma species research and implications
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cardiovascular Health and Disease Prevention
- Crystallization and Solubility Studies
- Calpain Protease Function and Regulation
- Chronic Lymphocytic Leukemia Research
- Synthesis and Biological Evaluation
- Synthesis and Characterization of Heterocyclic Compounds
- X-ray Diffraction in Crystallography
- Herpesvirus Infections and Treatments
- Polyamine Metabolism and Applications
- Dupuytren's Contracture and Treatments
- Tendon Structure and Treatment
- Lung Cancer Treatments and Mutations
- Economic and Financial Impacts of Cancer
- Cancer and Skin Lesions
- Autophagy in Disease and Therapy
- Research on Leishmaniasis Studies
University of Georgia
2002-2024
Stanford University
2021-2022
Princess Alexandra Hospital
2021
Fiona Stanley Fremantle Hospitals Group
2021
Beth Israel Deaconess Medical Center
2020-2021
Fiona Stanley Hospital
2019-2021
Fremantle Hospital
2021
University of Utah
2018-2020
George E. Wahlen Department of VA Medical Center
2020
Veterans Health Administration
2018-2020
Nucleotide metabolic pathways provide numerous successful targets for antiparasitic chemotherapy, but the human pathogen Cryptosporidium parvum thus far has proved extraordinarily refractory to classical treatments. Given importance of this protist as an opportunistic afflicting immunosuppressed individuals, effective treatments are urgently needed. The genome sequence C. is approaching completion, and we have used resource critically assess nucleotide biosynthesis a target in . Genomic...
A high-throughput growth assay for the protozoan parasite Toxoplasma gondii was developed based on a highly fluorescent transgenic line. These parasites are stably transfected with tandem yellow protein (YFP) and 1,000 times more than wild type. Parasites were inoculated in optical-bottom 384-well culture plates containing confluent monolayer of host cells, monitored by using fluorescence plate reader. The signal linearly correlated numbers over wide array. Direct comparison YFP...
ABSTRACT We have identified two novel proteins that colocalize with the s ub p ellicular m icrotubules in protozoan parasite Toxoplasma gondii and named these SPM1 SPM2. These basic isoelectric points both homologs other apicomplexan parasites. contains six tandem copies of a 32-amino-acid repeat, whereas SPM2 lacks defined protein signatures. Alignment apparent Plasmodium indicates greatest degree conservation lies carboxy-terminal half protein. Analysis while central repeats expanded to...
The asexually proliferating stages of apicomplexan parasites cause acute symptoms diseases such as malaria, cryptosporidiosis and toxoplasmosis. These are characterized by the presence two independent microtubule organizing centers (MTOCs). Centrioles found at poles intranuclear spindle. apical polar ring (APR), a MTOC unique to apicomplexans, organizes subpellicular microtubules which impose cell shape polarity on these protozoa. Here we describe characteristics novel protein that localizes...
Abstract Calcium ions regulate a diversity of cellular functions in all eukaryotes. The cytosolic Ca 2+ concentration is tightly regulated at the physiological 50–100 n m . Toxoplasma gondii genome predicts presence several genes encoding potential channels, pumps, and transporters. Many these are weakly expressed likely due to their impact physiology cell. Endogenous tagging has been widely used localize proteins T. but low level expression many them makes visualization tags difficult...
Abstract Dinitroanilines (oryzalin, trifluralin, ethafluralin) disrupt microtubules in protozoa but not vertebrate cells, causing selective death of intracellular Toxoplasma gondii parasites without affecting host cells. Parasites containing α1-tubulin point mutations are dinitroaniline resistant show increased rates aberrant replication relative to wild-type parasites. T. bearing the F52Y mutation were previously demonstrated spontaneously acquire two intragenic that decrease both...
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The contracted tissues from clubfeet resemble other fibroproliferative disorders such as palmar fibromatosis. Beta-catenin-mediated signaling is a crucial pathway controlling the response in many disorders. To determine if beta-catenin plays role clubfoot, and less were studied using Western analysis to protein level of beta-catenin. Primary cell cultures established these tissues, they treated with either lithium increase or Dickkopf-1 inhibit RNA was extracted cells analyzed how regulates...
ABSTRACT The human parasite Toxoplasma gondii is sensitive to dinitroaniline compounds which selectively disrupt microtubules in diverse protozoa but have no detectable effect on vertebrate host cell or other functions. Replication of wild-type T. inhibited by 0.5 2.5 μM oryzalin, mutant parasites harboring amino acid substitutions the predicted binding site confer resistance up 40 oryzalin. However, precise interaction between dinitroanilines and α-tubulin remains unclear. We investigated...
Select soft tissues in clubfeet are contracted, resulting stiffness. These contracted share ultrastructural characteristics with palmar fibromatosis (Dupuytren contracture), which the growth factors transforming factor-β (TGF-β) and platelet-derived factor (PDGF) expressed play a role regulating cell behavior. More tissue (medial side of foot) less (lateral from 20 were studied using reverse transcription-polymerase chain reaction western analysis for expression TGF-β PDGF (along collagen...
Based on crystallographic data of the complexes 2-alkyl(amino)ethyl-1,1-bisphosphonates-Trypanosoma cruzi farnesyl diphosphate synthase, some linear 1,1-bisphosphonic acids and other closely related derivatives were designed, synthesized biologically evaluated against T. cruzi, responsible agent Chagas disease Toxoplasma gondii, etiologic toxoplasmosis also towards target enzymes pyrophosphate synthase (TcFPPS) T gondii (TgFPPS), respectively. The isoprenoid-containing 1,1-bisphosphonates...
Abstract Two obligate intracellular parasites, Trypanosoma cruzi , the agent of Chagas disease, and Toxoplasma gondii an toxoplasmosis, upregulate mevalonate pathway their host cells upon infection, which suggests that this could be a potential drug target. In work, number compounds structurally related to WC‐9 (4‐phenoxyphenoxyethyl thiocyanate), known squalene synthase inhibitor, were designed, synthesized, evaluated for effect on T. growth in tissue culture cells. fluorine‐containing...
Key Points Circulating endothelial cell-specific molecule-1 (Esm-1) inversely correlates with diabetic kidney disease. Addition of Esm-1 in susceptible mice reduces albuminuria, and deletion resistant mildly worsens albuminuria. attenuates podocyte injury select IFN signaling, highlighting innate immunity as a potential mechanism Background Diabetic disease (DKD) is the most common cause failure world, novel predictive biomarkers molecular mechanisms are needed. Endothelial secreted...
Ca 2+ signaling in cells begins with the opening of channels either plasma membrane (PM) or endoplasmic reticulum (ER) and results a dramatic increase physiologically low (<100 nM) cytosolic level. The temporal spatial levels are well regulated to enable precise specific activation critical biological processes. regulates pathogenic features apicomplexan parasites like Toxoplasma gondii which infects approximately one-third world’s population. T. relies on signals stimulate traits its...
Ca 2+ signaling in cells begins with the opening of channels either plasma membrane (PM) or endoplasmic reticulum (ER) and results a dramatic increase physiologically low (<100 nM) cytosolic level. The temporal spatial levels are well regulated to enable precise specific activation critical biological processes. regulates pathogenic features apicomplexan parasites like Toxoplasma gondii which infects approximately one-third world’s population. T. relies on signals stimulate traits its...