A5067498884- Trace Elements in Health
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Mosquito-borne diseases and control
- Malaria Research and Control
- Antibiotic Resistance in Bacteria
- Parasitic Infections and Diagnostics
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- HIV/AIDS drug development and treatment
- Enzyme Structure and Function
- Corrosion Behavior and Inhibition
- Fungal and yeast genetics research
- Rabies epidemiology and control
- Protein Structure and Dynamics
- Arsenic contamination and mitigation
- Plant Micronutrient Interactions and Effects
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Infections and bacterial resistance
- Data Mining Algorithms and Applications
- Nitric Oxide and Endothelin Effects
- Cytomegalovirus and herpesvirus research
- Antibiotic Use and Resistance
University of Michigan–Ann Arbor
2017-2025
University of Glasgow
2022
Clemson University
2022
Argonne National Laboratory
2022
Harding University Main Campus
2022
Johns Hopkins University
2017-2019
University of Camagüey
2019
University of California, Irvine
2018
University of Virginia
2018
University of Massachusetts Amherst
2018
Abstract Iron is essential to cells as a cofactor in enzymes of respiration and replication, however without correct storage, iron leads the formation dangerous oxygen radicals. In yeast plants, transported into membrane-bound vacuole by vacuolar transporter (VIT). This conserved apicomplexan family obligate intracellular parasites, including Toxoplasma gondii . Here, we assess role VIT storage T. By deleting VIT, find slight growth defect vitro, hypersensitivity, confirming its parasite...
Streptococcus pneumoniae adhesin competence regulator (AdcR), the first metal-dependent member of multiple antibiotic resistance (MarR) family proteins, represses transcription a high-affinity zinc-specific uptake transporter, group surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD, and PhtE), an AdcA homologue (AdcAII). The 2.0 Å resolution structure Zn(II)-bound AdcR reveals highly helical two-fold-symmetric dimer with two distinct metal-binding sites per...
Acute virulence in Toxoplasma gondii is linked to an excessive proinflammatory cytokine cascade during laboratory murine infection. Previous work showed that T. secretes a pore forming protein, PLP1, required for efficient cytolytic egress from host cells. Deletion of the PLP1 gene results defective infected culture cells and marked reduction parasite virulence. The goal present study was gain insight into nature attenuated observed knockout compared wild type (WT) RH parasites. Using vivo...
A metal-mediated interprotomer hydrogen bond has been implicated in the allosteric mechanism of DNA operator binding several metal-sensing proteins. Using computational methods, we investigate energetics such zinc-mediated interactions members ArsR/SmtB family proteins (CzrA, SmtB, CadC, and NmtR) MarR zinc-uptake repressor AdcR, which feature similar interactions, but sites that differ widely their responsiveness. We provide novel structural insight into previously uncharacterized forms...
Abstract Secretory proteins are of particular importance to apicomplexan parasites and comprise over 15% the genomes human pathogens that cause diseases like malaria, toxoplasmosis babesiosis as well other agricultural significance. Here, we developed an approach allows us control trafficking destination secretory in malaria parasite Plasmodium falciparum . Based on unique structural requirements apicoplast transit peptides, designed three conditional localization domains (CLD1, 2 3) can be...
Intracellular pathogens must egress from the host cell to continue their infectious cycle. Apicomplexans are a phylum of intracellular protozoans that have evolved members membrane attack complex and perforin (MACPF) family pore forming proteins disrupt cellular membranes for traversing cells during tissue migration or replicative vacuole following reproduction. Previous work showed apicomplexan Toxoplasma gondii secretes perforin-like protein (TgPLP1) contains C-terminal Domain (CTD) which...
With roughly 2 billion people infected, the neurotropic protozoan Toxoplasma gondii remains one of most pervasive and infectious parasites. infection is second leading cause death due to foodborne illness in United States, causes severe disease immunocompromised patients, correlated with several cognitive neurological disorders. Currently, no therapies exist that are capable eliminating persistent central nervous system (CNS). In this study we report identification triazine nitrile...
Lipoate is an essential cofactor for enzymes that are important central metabolism and other processes. In malaria parasites, scavenged lipoate from the human host required survival. The Plasmodium falciparum mitochondrion contains two (PfLipL1 PfLipL2) responsible activating mitochondrial proteins through covalent attachment of (lipoylation). Lipoylation occurs via a novel redox-gated mechanism remains poorly understood. We show PfLipL1 functions as redox switch determines which downstream...
Heberprovac is a GnRH based vaccine candidate containing 2.4mg of the GnRHm1-TT peptide as main active principle; 245ug very small size proteoliposomes adjuvant (VSSP); and 350uL Montanide ISA 51 VG oil adjuvant. The aim this study was to assess in advanced prostate cancer patients safety tolerance Heberprovac, its capacity induce anti-GnRH antibodies, turn, effects on testosterone production, PSA values patient survival. Methods Eight with indication for hormonal therapy...
Glutamine is the preferred source of nitrogen Neurospora crassa. In its presence and that gene product MS5 (nmr-1), fungus represses assimilation less forms nitrogen, such as nitrate. absence glutamine nit-2, are assimilated long a specific pathway for their induced. We report here isolation, from cosmid bank, complements can also complement nit-2. speculate this result suggests an interaction between nit-2 products important in regulation nitrate assimilation.
Abstract Iron is essential to living cells, acting as a cofactor in number of enzymes metabolism; however, iron requires proper storage or it can be dangerous the cell. In both yeast and plants, stored vacuole through action vacuolar transporter (VIT). This conserved apicomplexan family obligate intracellular parasites, including Toxoplasma gondii , pathogen medical veterinary importance. Here, we assess role VIT T. . We show that deletion causes slight growth defect vitro however leads...
Plasmodium falciparum lipoate protein ligase 1 (PfLipL1) is an ATP-dependent that belongs to the biotin/lipoate A/B family (PFAM PF03099). PfLipL1 only known canonical in Pf and functions as a redox switch between two lipoylation routes parasite mitochondrion. Here, we report crystal structure of deletion construct (PfLipL1Δ243-279 ) bound lipoate, validate activity this both vitro assay cell-based assay. This characterization represents first step understanding dependence mechanism malaria...
Abstract Iron is essential to living cells, acting as a cofactor in number of important enzymes metabolism; however the absence correct storage iron forms dangerous oxygen radicals. In both yeast and plants, stored membrane-bound vacuole through action vacuolar transporter (VIT). This conserved apicomplexan family obligate intracellular parasites, including Toxoplasma gondii , pathogen medical veterinary importance. Here, we assess role VIT, storage, T. gondii. We show that restricted...
Abstract Intracellular pathogens must egress from the host cell to continue their infectious cycle. Apicomplexans are a phylum of intracellular protozoans that have evolved members membrane attack complex and perforin (MACPF) family pore forming proteins disrupt cellular membranes for traversing cells during tissue migration or replicative vacuole following reproduction. Previous work showed apicomplexan Toxoplasma gondii secretes perforin-like protein ( Tg PLP1) contains C-terminal Domain...
Glutamine is the preferred source of nitrogen Neurospora crassa. In its presence and that gene product MS5 (nmr-1), fungus represses assimilation less forms nitrogen, such as nitrate. absence glutamine nit-2, are assimilated long a specific pathway for their induced. We report here isolation, from cosmid bank, complements can also complement nit-2. speculate this result suggests an interaction between nit-2 products important in regulation nitrate assimilation.