Membranes from highly purified natural killer (NK) cells were ADP-ribosylated by treatment with cholera toxin (CTX). CTX resulted in a single band of specific 32P incorporation at Mr 43,600. intact NK caused 9-fold increase cyclic AMP (cAMP) concentrations. Pretreatment diminished their ability to lyse K562 tumour up 79%. Forskolin elevated cell cAMP levels 8-fold and decreased lysis 45%. Adrenaline isoprenaline (isoproterenol) both inhibited approx. 35% least 2.5-fold, inhibition was...

Human natural killer (NK) cells are lymphocytes that destroy tumor cells, virally-infected and antibody-coated cells. Tetrabromobisphenol A (TBBPA) is used both as a reactive an additive flame retardant in variety of materials appears to contaminate the environment. TBBPA has been found human blood samples if it interferes with NK cell function, this could increase risk development and/or viral infection. The present study examines effects exposure various concentrations for 24 hr, 48 6 days...

ABSTRACT Pentachlorophenol (PCP) and dibutyltin dichloride (DBT) contaminate the environment due to their diverse applications. PCP has been found from 0.26 5 μM in serum of exposed individuals at an average 0.15 unexposed. DBT detected human blood levels up 0.3 μM. Exposure these contaminants is linked pathological conditions including cancer. Interleukin‐1 beta (IL‐1β) IL‐6 are pro‐inflammatory cytokines that when produced inappropriately can cause chronic inflammation, which pathologies...

Human natural killer (NK) cells are a first-line immune defense against tumor and virally-infected cells. If their function is impaired, it leaves an individual more susceptible to cancer development or viral infection. The ability of compounds that contaminate the environment suppress NK could contribute increased risk development. There wide spectrum significantly water food consumed by humans, leading accumulation some these in human tissues. In current study, we examined three such...

Ovarian cancer (OC) has the highest mortality rate among gynecological malignancies.Because chemokine network is involved in OC progression, we evaluated associations between expression and survival tumor suppressor protein p53 (TP53) wildtype (TP53WT) mutant (TP53m) datasets.TP53 was highly mutated compared to other types.Among subtypes, CXCL14 predominantly expressed clear cell OC, CCL15 CCL20 mucinous OC.TP53WT endometrioid TP53m, showing better progression-free but no difference overall...

Abstract Human natural killer (NK) lymphocytes play a central role in immune system defense against viral infection and the formation of primary tumors. Organotin (OT) pesticides have been used industrial agricultural applications, OT contamination has reported water, sediment, fish. Carbamate are currently chemicals. Two specific carbamates agriculture ziram maneb; also is as an additive rubber products including latex gloves. In previous studies we demonstrated that at concentrations...

Abstract The purpose of this study was to investigate the role that tributyltin (TBT)‐induced decreases in ATP levels may play TBT‐induced tumor lysing (lytic) function natural killer (NK) cells. NK cells are a subset lymphocytes act as an initial immune defense against and virally infected TBT is environmental contaminant has been detected human blood, which shown interfere with synthesis. Previous studies have able decrease very significantly lytic In were exposed various concentrations...

ABSTRACT Butyltins (BTs) have been in widespread use. Tributyltin (TBT) has used as a biocide variety of applications and is found human blood samples. Dibutyltin (DBT) stabilizer polyvinyl chloride plastics de‐worming agent poultry. DBT, like TBT, blood. Human natural killer (NK) cells are the earliest defense against tumors viral infections secrete cytokine tumor necrosis factor‐alpha (TNF‐α). TNF‐α an important regulator adaptive innate immune responses. promotes inflammation association...

Abstract Tributyltin (TBT) has been used as a biocide in industrial applications such wood preservation, antifouling paint and antifungal agents. Owing to its many uses, it contaminates the environment found human blood samples. Interleukin‐1 beta (IL‐1β) is pro‐inflammatory cytokine that promotes cell growth, tissue repair immune response regulation. Produced predominately by both monocytes macrophages, IL‐1β appears increase invasiveness of certain tumors. This study shows TBT modifies...

Organochlorine pesticides are used worldwide. To our knowledge there have been no studies dealing with the effects of these agents under in vitro conditions on human natural killer (NK) cell cytotoxic function. NK cells play a central role immune defense against tumor development and viral infections. Thus, any agent that interferes ability to lyse their targets could increase risk incidence and/or In this study, we examined organochlorine some breakdown products cells. A total 11 compounds...

Human natural killer (NK) lymphocytes are able to destroy tumor cells and virally-infected cells. Interference with their function can leave an individual increased susceptibility cancer development and/or viral infection. We have shown that the tumor-destroying (lytic) of NK be dramatically decreased by exposure environmental contaminant tetrabromobisphenol A (TBBPA). TBBPA is a flame retardant used in variety materials including circuit boards, carpeting, upholstery has been found human...

Tributyltin (TBT) and dibutyltin (DBT) are widespread environmental contaminants found in food, beverages, human blood samples. Both of these butyltins (BTs) interfere with the ability natural killer (NK) cells to lyse target alter secretion pro-inflammatory cytokine tumor necrosis factor alpha (TNFα) from immune vitro. The capacity BTs other cytokines has not been examined. Interferon gamma (IFNγ) is a modulator adaptive innate responses, playing an important role overall competence. This...