A5007693875- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Glycosylation and Glycoproteins Research
- Immune responses and vaccinations
- Probiotics and Fermented Foods
- Medical Imaging and Pathology Studies
- T-cell and B-cell Immunology
- Parasitic Diseases Research and Treatment
- Peptidase Inhibition and Analysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nail Diseases and Treatments
University of Aberdeen
2012-2023
Medical Research Council
2018-2019
Pfizer (United Kingdom)
2012
Candida albicans is a major life-threatening human fungal pathogen. Host defence against systemic infection relies mainly on phagocytosis of cells by the innate immune system. In this study, we have employed video microscopy, coupled with sophisticated image analysis tools, to assess contribution distinct C. cell wall components and yeast-hypha morphogenesis specific stages macrophages. We show that macrophage migration towards was dependent glycosylation status wall, but not viability or...
Abstract The high global burden of over one million annual lethal fungal infections reflects a lack protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated unique set fully human anti- Candida monoclonal antibodies (mAbs) with diagnostic therapeutic potential by expressing recombinant from genes cloned the B cells patients suffering candidiasis. Single class switched memory isolated donors serum-positive for IgG were differentiated in vitro screened against...
ABSTRACT An important first line of defense against Candida albicans infections is the killing fungal cells by professional phagocytes innate immune system, such as polymorphonuclear (PMNs) and macrophages. In this study, we employed live-cell video microscopy coupled with dynamic image analysis tools to provide insights into complexity C. phagocytosis when macrophages PMNs were incubated alone both phagocyte subsets present. When only one subtype, had a lower overall phagocytic capacity...
Phagocytosis is an essential component of our immune defence against fungal pathogens. Differences in the dynamics phagocyte migration, recognition, uptake and phagolysosome maturation are dependent on characteristics cargo, particular to differences cell wall composition cellular morphology. However, studies that have focused interactions with individual species not enabled comparisons kinetics these be made between different species. We therefore used live video microscopy examine temporal...
Phagocytosis is an essential component of our immune defence against fungal pathogens. Differences in the dynamics phagocyte migration, recognition, uptake and phagolysosome maturation are dependent on characteristics cargo, particular to differences cell wall composition cellular morphology. However, studies that have focused interactions with individual species not enabled comparisons kinetics these be made between different species. We therefore used live video microscopy examine temporal...