A5069553677- Radiation Therapy and Dosimetry
- Ion-surface interactions and analysis
- DNA Repair Mechanisms
- X-ray Spectroscopy and Fluorescence Analysis
- Molecular Biology Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Effects of Radiation Exposure
- Nanoparticles: synthesis and applications
- Nuclear Physics and Applications
- Photoacoustic and Ultrasonic Imaging
- Radiation Effects in Electronics
- Bone Tissue Engineering Materials
- Radiation Detection and Scintillator Technologies
- PARP inhibition in cancer therapy
- Advanced Radiotherapy Techniques
- Electrochemical Analysis and Applications
- Nanoparticle-Based Drug Delivery
- Microfluidic and Bio-sensing Technologies
- Genetics, Aging, and Longevity in Model Organisms
- Diamond and Carbon-based Materials Research
- Biofield Effects and Biophysics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Radiation Effects and Dosimetry
- Plant Genetic and Mutation Studies
- Carcinogens and Genotoxicity Assessment
Université de Bordeaux
2010-2024
Centre National de la Recherche Scientifique
2011-2024
Laboratoire de Physique des deux infinis Bordeaux
2015-2024
Institut National de Physique Nucléaire et de Physique des Particules
2004-2021
Institut de Recherches en Technologies et Sciences pour le Vivant
2014
GSI Helmholtz Centre for Heavy Ion Research
2006
GfK (Germany)
2005
On behalf of the Geant4 Standard and Low-Energy Electromagnetic Physics Working Groups.The authors are members Geant4-DNA
Abstract The reliance of all cell types on the mitochondrial function for survival makes mitochondria an interesting target when trying to understand their role in cellular response ionizing radiation. By harnessing highly focused carbon ions and protons using microbeams, we have performed situ live imaging targeted irradiation individual stained with Tetramethyl rhodamine ethyl ester (TMRE), a cationic fluorophore which accumulates electrophoretically polarized mitochondria. Targeted both...
Deciphering the molecular basis of toxicology mechanism induced by nanoparticles (NPs) remains an essential challenge. Ion Beam Analysis (IBA) was applied in combination with Transmission Electron Microscopy and Confocal to analyze human keratinocytes exposed TiO(2)-NPs. Investigating chemical elemental distributions using IBA gives rise a fine quantification TiO(2)-NPs uptake within cell determination intracellular modifications after internalization. In addition, fluorescent dye-modified...
Fournier, C., Barberet, P., Pouthier, T., Ritter, S., Fischer, B., Voss, K. O., Funayama, Hamada, N., Kobayashi, Y. and Taucher-Scholz, G. No Evidence for DNA Early Cytogenetic Damage in Bystander Cells after Heavy-Ion Microirradiation at Two Facilities. Radiat. Res. 171, 530–540 (2009).The occurrence of bystander effects has challenged the evaluation risk heavy ions, mainly context space exploration increasing application carbon ions radiotherapy. In present study, we addressed whether...
Although titanium dioxide nanoparticles (TiO2 NPs) have been extensively studied, their possible impact on health due to specific properties supported by size and geometry, remains be fully characterized support risk assessment. To further document NPs biological effects, we investigated the of TiO2 morphology outcomes. this end, were synthesized as nanoneedles (NNs), titanate scrolled nanosheets (TNs), gel-sol-based isotropic (INPs) tested for perturbation cellular homeostasis (cellular ion...
Evidence has accumulated that irradiated cells affect their unirradiated neighbors, so they in turn display cellular responses typically associated with direct radiation exposure. These are generally known as bystander effects. In this study, cell cycle-related were investigated three strains of human fibroblasts after exposure to densely ionizing radiation. Varying the linear energy transfer (LET) from 11 15,000 keV microm(-1) allowed a study impact complexity DNA damage inducing on cells....
Assessing in situ nanoparticles (NPs) internalization at the level of a single cell is difficult but critical task due to their potential use nanomedicine. One main actual challenges control number internalized NPs per cell. To detect, track, and above all quantify cell, we propose an approach based on multimodal correlative microscopy (MCM), via complementarity three imaging techniques: fluorescence (FM), scanning electron (SEM), ion beam analysis (IBA). This MCM was performed targeted...
Hadron therapy is an innovative mode of radiotherapy (RT) for cancer treatment which enables tumor cells to be more effectively destroyed than conventional RT using photons. The precise knowledge the lineal energy particles used in field microdosimetry (MKM model) as a fundamental parameter prediction relative biological efficiency (RBE) clinical beams. Based on single‐crystal CVD (scCVD) super‐thin diamond membranes obtained deep Ar/O 2 plasma etching, prototypes solid‐state microdosimeters...
Cell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, dynamic change under specific environmental stresses. This work reports on the remote mapping of 3D morphology with an in-plane resolution limited by optics out-of-plane accuracy down to a tenth optical wavelength. For this, GHz coherent acoustic phonons their resonance harmonics were tracked means ultrafast opto-acoustic technique. After illustrating...
How DNA damage and repair processes affect the biomechanical properties of nucleus interior remains unknown. Here, an opto-acoustic microscope based on time-domain Brillouin spectroscopy (TDBS) was used to investigate induced regulation intra-nuclear mechanics. With this ultrafast pump-probe technique, coherent acoustic phonons were tracked along their propagation in intra-nucleus nanostructure complex stiffness moduli thicknesses measured with optical resolution. Osteosarcoma cells exposed...
Light-ion microbeams provide a unique opportunity to irradiate biological samples at the cellular level and investigate radiobiological effects low doses of high linear energy transfer ionizing radiation. Since 1998 single-ion irradiation facility has been developed on focused horizontal microbeam line CENBG 3.5 MV Van de Graaff accelerator. This setup delivers in air single protons alpha particles few MeV onto cultured cells, with spatial resolution micrometers, allowing subcellular...
Abstract As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted therapy. A better understanding α-particle induced damage at the DNA scale can be achieved by following their tracks real-time living cells. Focused microbeams facilitate this but, due to low energy (up few MeV) and limited range, detection, delivery, follow-up observations radiation-induced remain difficult. In study, we developed thin Boron-doped...
Charged-particle microbeams (CPMs) provide a unique opportunity to investigate the effects of ionizing radiation on living biological specimens with precise control delivered dose, i.e. number particles per cell. We describe methodology manipulate and micro-irradiate early stage C. elegans embryos at specific phase cell division controlled dose using CPM. To validate this approach, we observe radiation-induced damage, such as reduced mobility, incomplete appearance chromatin bridges during...
We report the development of thin single crystal diamond membranes suitable for dose control in targeted cell irradiation experiments with a proton microbeam. A specific design was achieved to deliver protons hit detection efficiency approaching 100%. The have thicknesses between 1.8 and 3 μm are used as vacuum windows on microbeam line. impact these transmission detectors spot size is estimated by Monte-Carlo simulations, indicating that beam lateral resolution below 2 achieved. This...
A single crystal chemical vapor deposition diamond-based microdosimeter prototype featuring an array of micro-sensitive volumes (μSVs) and surrounded by a so-called guard ring (GR) electrode has been fabricated using various microfabrication techniques available at Diamond Sensors Laboratory CEA, Saclay. The GR was irradiated raster scanning method with 2 MeV proton microbeams. charge transport properties the sensor were determined sub-micron spatial resolution measuring collection...
We describe a methodology to manipulate Caenorhabditis elegans (C. elegans) and irradiate the stem progenitor gonad region using three MeV protons at specific developmental stage (L1). The consequences of targeted irradiation were first investigated by considering organogenesis vulva gonad, two well-defined characterized systems in C. elegans. In addition, we adapted high-throughput analysis protocols, cell-sorting assays (COPAS) whole transcriptome analysis, limited number worms (>300)...
Abstract Because of the large natural inter‐individual variability and some difficulties in obtaining material from surgery or healthy volunteers, use human native skin experimentation comes up against fierce competition experimental models, such as reconstituted epidermis grafted animals. In this study, micro‐PIXE was used association with other ion beam microanalysis techniques to characterize different models on a microscopic scale. The ionic species were found be highly compartmentalized...