A5101929041- Fungal and yeast genetics research
- Arsenic contamination and mitigation
- Trace Elements in Health
- Nuclear Structure and Function
- RNA Research and Splicing
- Heavy Metal Exposure and Toxicity
- Heat shock proteins research
University of Gothenburg
2017-2021
Cadmium is a highly poisonous metal and classified as human carcinogen. While its toxicity undisputed, the underlying in vivo molecular mechanisms are not fully understood. Here, we demonstrate that cadmium induces aggregation of cytosolic proteins living Saccharomyces cerevisiae cells. primarily targets process synthesis or folding, probably by interacting with exposed thiol groups not-yet-folded proteins. On basis vitro data, show cadmium-aggregated form seeds increase misfolding other...
Significance A defining feature of eukaryotes is the nuclear envelope, a double lipid bilayer that serves to isolate and protect cell’s genetic material. Transport large molecules over this barrier believed occur almost exclusively via pores. However, herpes virions mega-ribonucleoproteins (megaRNPs) use an alternative means transport—via envelope budding (NEB). Here, we show NEB ubiquitous eukaryotic phenomenon increases when exposed various forms cellular stress. frequency was maximal cell...
The toxic metalloid arsenic causes widespread misfolding and aggregation of cellular proteins. How these protein aggregates are formed in vivo, the mechanisms by which they affect cells how prevent their accumulation is not fully understood. To find components involved processes, we performed a genome-wide imaging screen identified Saccharomyces cerevisiae deletion mutants with either enhanced or reduced levels during arsenite exposure. We show that many factors crucial to safeguard...
Abstract Nuclear envelope budding (NEB) is a recently discovered alternative pathway for nucleocytoplasmic communication distinct from the movement of material through nuclear pore complex. Through quantitative electron microscopy and tomography, we demonstrate how NEB evolutionarily conserved early protists to human cells. In yeast Saccharomyces cerevisiae , events occur with higher frequency during heat shock, upon exposure arsenite or hydrogen peroxide, when proteasome inhibited. Yeast...
ABSTRACT Exposure to toxic metals and metalloids such as cadmium arsenic results in widespread misfolding aggregation of cellular proteins. How these protein aggregates are formed vivo , the mechanisms by which they affect cells, how cells prevent their accumulation during environmental stress is not fully understood. To find components involved processes, we performed a genome-wide imaging screen identified yeast deletion mutants with either enhanced or reduced levels arsenite exposure....