A5002707395- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Mitochondrial Function and Pathology
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Parkinson's Disease Mechanisms and Treatments
- Bacteriophages and microbial interactions
- Nuclear Structure and Function
- Antibiotic Resistance in Bacteria
- Autophagy in Disease and Therapy
- Protein Structure and Dynamics
- Cellular transport and secretion
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Signaling Pathways in Disease
- Antimicrobial Peptides and Activities
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Neonatal and Maternal Infections
- Cannabis and Cannabinoid Research
- Coenzyme Q10 studies and effects
- RNA regulation and disease
- Neurological diseases and metabolism
Umeå University
2023-2024
Stockholm University
2019-2024
University of Graz
2016-2023
Wenner-Gren Foundations
2018-2020
BioTechMed-Graz
2018-2019
University Medical Center Freiburg
2019
Berliner Hochschule für Technik
2019
Nawi Graz
2016
University of Missouri
1993
Respiratory chains are crucial for cellular energy conversion and consist of multi-subunit complexes that can assemble into supercomplexes. These structures have been intensively characterized in various organisms, but their physiological roles remain unclear. Here, we elucidate function by leveraging a high-resolution structural model yeast respiratory supercomplexes allowed us to inhibit supercomplex formation mutation key residues the interaction interface. Analyses mutant defective...
Significance A defining feature of eukaryotes is the nuclear envelope, a double lipid bilayer that serves to isolate and protect cell’s genetic material. Transport large molecules over this barrier believed occur almost exclusively via pores. However, herpes virions mega-ribonucleoproteins (megaRNPs) use an alternative means transport—via envelope budding (NEB). Here, we show NEB ubiquitous eukaryotic phenomenon increases when exposed various forms cellular stress. frequency was maximal cell...
Abstract The resilience of cellular proteostasis declines with age, which drives protein aggregation and compromises viability. nucleus has emerged as a key quality control compartment that handles misfolded proteins produced by the cytosolic biosynthesis system. Here, we find age-associated metabolic cues target yeast disaggregase Hsp104 to maintain functional nuclear proteome during quiescence. switch respiratory metabolism accompanying decrease in translation rates direct interact latent...
Membrane contact sites facilitate the exchange of metabolites between organelles to support interorganellar communication. The nucleus-vacuole junctions (NVJs) establish physical perinuclear endoplasmic reticulum (ER) and vacuole. Although NVJ tethers are known, how abundance composition controlled in response metabolic cues remains elusive. Here, we identify ER protein Snd3 as central factor for formation. interacts with tethers, supports their targeting contacts, is essential Upon glucose...
The nuclear pore complex (NPC) has long been assumed to be the sole route across envelope, and under normal homeostatic conditions it is indeed main mechanism of nucleo-cytoplasmic transport. However, also known that e.g. herpesviruses cross envelope utilizing a pathway entitled egress or envelopment/de-envelopment. Despite this, thread observations suggests mechanisms similar viral may transiently used in healthy cells. It since proposed like budding (NEB) can facilitate transport RNA...
Folding of newly synthesized proteins poses challenges for a functional proteome. Dedicated protein quality control (PQC) systems either promote the folding nascent polypeptides at ribosomes or, if this fails, ensure their degradation. Although well studied cytosolic biogenesis, it is not understood how these processes work mitochondrially encoded proteins, key subunits oxidative phosphorylation (OXPHOS) system. Here, we identify dedicated hubs in proximity to mitoribosomal tunnel exits...
Abstract Untreatable bacterial infections caused by a perpetual increase of antibiotic resistant strains represent serious threat to human healthcare in the 21 st century. Conjugative DNA transfer is most important mechanism for resistance and virulence gene dissemination among bacteria mediated protein complex, known as type IV secretion system (T4SS). The core T4SS multiprotein complex that spans envelope channel macromolecular secretion. We report NMR structure functional characterization...
The degeneration of dopaminergic neurons during Parkinson's disease is intimately linked to malfunction α-synuclein, the main component proteinaceous intracellular inclusions characteristic for this pathology. cytotoxicity α-synuclein has been attributed disturbances in several biological processes conserved from yeast humans, including Ca2+ homeostasis, general lysosomal function, and autophagy. However, precise sequence events that eventually results cell death remains unclear. Here, we...
A functional proteome is essential for life and maintained by protein quality control (PQC) systems in the cytosol organelles. Protein aggregation an indicator of a decline PQC linked to aging disease. Mitochondrial critical maintain mitochondrial function thus cellular fitness. How mitochondria handle aggregated proteins not well understood. Here we tested how metabolic status impacts on formation clearance aggregates within yeast assessed which are particularly sensitive denaturation.
Abstract Overexposure to manganese disrupts cellular energy metabolism across species, but the molecular mechanism underlying toxicity remains enigmatic. Here, we report that excess selectively coenzyme Q (CoQ) biosynthesis, resulting in failure of mitochondrial bioenergetics. While respiratory chain complexes remain intact, lack CoQ as lipophilic electron carrier precludes oxidative phosphorylation and leads premature cell organismal death. At a level, overload causes mismetallation...
Mitochondrial dysfunction is a prominent trait of cellular decline during aging and intimately linked to neuronal degeneration Parkinson's disease (PD). Various proteins associated with PD have been shown differentially impact mitochondrial dynamics, quality control function, including the leucine-rich repeat kinase 2 (LRRK2). Here, we demonstrate that high levels enzymatic core human LRRK2, harboring GTPase as well activity, decreases mass via an impairment biogenesis in yeast. We link...
The dissemination of multi-resistant bacteria represents an enormous burden on modern healthcare. Plasmid-borne conjugative transfer is the most prevalent mechanism, requiring a type IV secretion system that enables to spread beneficial traits, such as resistance last-line antibiotics, among different genera. Inc18 plasmids, like Gram-positive broad host-range plasmid pIP501, are substantially involved in propagation vancomycin from Enterococci methicillin-resistant strains Staphylococcus...
Nutrient starvation initiates cell cycle exit and entry into quiescence, a reversible, non-proliferative state characterized by stress tolerance, longevity large-scale remodeling of subcellular structures. Depending on the nature depleted nutrient, yeast cells are assumed to enter heterogeneous quiescent states with unique but mostly unexplored characteristics. Here, we show that storage consumption neutral lipids in lipid droplets (LDs) differentially impacts regulation quiescence driven...