A5045604441- Fungal and yeast genetics research
- Heat shock proteins research
- Nuclear Structure and Function
- Endoplasmic Reticulum Stress and Disease
- DNA Repair Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Molecular Biology Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Advanced MRI Techniques and Applications
- Cardiac Imaging and Diagnostics
- AI in cancer detection
- RNA regulation and disease
- Lipid metabolism and biosynthesis
- Genetic Neurodegenerative Diseases
- Trace Elements in Health
- Ultrasound Imaging and Elastography
- Advanced Fluorescence Microscopy Techniques
- Hepatitis C virus research
- Genetics, Aging, and Longevity in Model Organisms
- MRI in cancer diagnosis
- Liver Disease Diagnosis and Treatment
University of Gothenburg
2018-2025
Dalian University of Technology
2023
Higher Colleges of Technology
2023
Sahlgrenska University Hospital
2022
Medical University of Vienna
2020
Vienna Biocenter
2020
Vienna BioCenter Core Facilities
2020
Recent advances in computing power triggered the use of artificial intelligence image analysis life sciences. To train these algorithms, a large enough set certified labeled data is required. The trained neural network then capable producing accurate instance segmentation results that will need to be re-assembled into original dataset: entire process requires substantial expertise and time achieve quantifiable results. speed-up process, from cell organelle detection quantification across...
Significance A defining feature of eukaryotes is the nuclear envelope, a double lipid bilayer that serves to isolate and protect cell’s genetic material. Transport large molecules over this barrier believed occur almost exclusively via pores. However, herpes virions mega-ribonucleoproteins (megaRNPs) use an alternative means transport—via envelope budding (NEB). Here, we show NEB ubiquitous eukaryotic phenomenon increases when exposed various forms cellular stress. frequency was maximal cell...
ABSTRACT Human exposure to arsenicals is associated with devastating diseases such as cancer and neurodegeneration. At the same time, arsenic-based drugs are used therapeutic agents. The ability of arsenic directly bind proteins correlated its toxic effects highlighting importance elucidating arsenic-protein interactions. In this study, we took a proteomic approach identified 174 that in Saccharomyces cerevisiae . Proteins involved nucleocytoplasmic transport were markedly enriched among...
The nuclear pore complex (NPC) has long been assumed to be the sole route across envelope, and under normal homeostatic conditions it is indeed main mechanism of nucleo-cytoplasmic transport. However, also known that e.g. herpesviruses cross envelope utilizing a pathway entitled egress or envelopment/de-envelopment. Despite this, thread observations suggests mechanisms similar viral may transiently used in healthy cells. It since proposed like budding (NEB) can facilitate transport RNA...
ABSTRACT When the temperature is increased, heat-shock response activated to protect cellular environment. The transcriptomics and proteomics of this process are intensively studied, while information about how cell responds structurally heat stress mostly lacking. Here, Saccharomyces cerevisiae were subjected a mild continuous shock (38°C) intermittently cryo-immobilised for electron microscopy. Through measuring changes in all distinguishable organelle numbers, sizes morphologies over 2100...
A major consequence of aging and stress, in yeast to humans, is an increased accumulation protein aggregates at distinct sites within the cells. Using genetic screens, immunoelectron microscopy, three-dimensional modeling our efforts elucidate importance aggregate annexation, we found that most accumulate near surface mitochondria. Further, show virus-like particles (VLPs), which are part retrotransposition cycle Ty elements, markedly enriched these aggregation. RNA interference-mediated...
The primary hepatic consequence of obesity is non-alcoholic fatty liver disease (NAFLD), affecting about 25% the global adult population. Non-alcoholic steatohepatitis (NASH) a severe form NAFLD characterized by lipid accumulation, inflammation, and hepatocyte ballooning, with different degree fibrosis. In light rapidly increasing prevalence NASH, there an urgent need for improved understanding molecular pathogenesis these diseases. aim this study was to decipher possible role STE20-type...
The accumulation of misfolded proteins is a hallmark aging and many neurodegenerative diseases, making it important to understand how the cellular machinery recognizes processes such proteins. A key question in this respect whether are handled similar way regardless their genetic origin. To approach question, we compared three different proteins, guk1-7, gus1-3, pro3-1, by cell. We show that all nontoxic, even though highly overexpressed, highlighting usefulness analyzing response misfolding...
Abstract When the temperature is increased, heat shock response activated to protect cellular environment. The transcriptomics and proteomics of this process are intensively studied, while information about how cell responds structurally stress mostly lacking. Here, Saccharomyces cerevisiae were subjected a mild continuous intermittently cryo-immobilized for electron microscopy. Through measuring changes in all distinguishable organelle numbers, sizes, morphologies over 2400 micrographs...
Abstract Nuclear envelope budding (NEB) is a recently discovered alternative pathway for nucleocytoplasmic communication distinct from the movement of material through nuclear pore complex. Through quantitative electron microscopy and tomography, we demonstrate how NEB evolutionarily conserved early protists to human cells. In yeast Saccharomyces cerevisiae , events occur with higher frequency during heat shock, upon exposure arsenite or hydrogen peroxide, when proteasome inhibited. Yeast...
Recent advances in computing power triggered the use of Artificial Intelligence image analysis life sciences. To train these algorithms, a large enough set certified labelled data is required. The trained neural network then capable producing accurate instance segmentation results, that will need to be re-assembled into original dataset: entire process requires substantial expertise and time achieve quantifiable results. speed-up process, from cell organelle detection quantification across...
ABSTRACT First Person is a series of interviews with the first authors selection papers published in Journal Cell Science, helping early-career researchers promote themselves alongside their papers. Katharina Keuenhof author on ‘ Large organellar changes occur during mild heat shock yeast’, JCS. PhD student lab Johanna Höög at Department Chemistry and Molecular Biology, Medicinaregatan, Göteborg, Sweden, where she curious about ultrastructural within biological organisms using electron...