Abstract The germline mutation rate has been extensively studied and found to vary greatly between species, but much less is known about the somatic in multicellular organisms, which remains very difficult determine. Here, we present data on rates mice humans, obtained by sequencing single cells clones derived from primary fibroblasts, allows us make first direct comparison with these two species. results indicate that almost orders of magnitude higher than both are significantly humans. Our...

Periodontitis is a dysbiotic infectious disease that leads to the destruction of tooth supporting tissues. There increasing evidence periodontitis may affect development and severity Alzheimer’s (AD). However, mechanism(s) by which periodontal infection impacts neurodegenerative process in AD remains unclear. In present study, using an amyloid precursor protein (APP) knock-in ( App KI) mouse model, we showed oral with Porphyromonas gingivalis (Pg), keystone pathogen periodontitis, worsened...

Different pulp capping materials have different origins and compositions, require preparations, may vary in their bioactive properties.

The six subunit ORC is essential for initiation of DNA replication in eukaryotes. Cancer cell-lines culture can survive and replicate after genetic inactivation individual subunits, ORC1, ORC2 or ORC5. In primary cells, ORC1 was dispensable the mouse liver endo-reduplication, but this could be explained by homolog, CDC6, substituting to restore functional ORC. Here, we have created mice with a conditional deletion ORC2, which does not homolog. Although embryo fibroblasts require...

Abstract In multiple myeloma, abnormal plasma cells accumulate and proliferate in the bone marrow. Recently, we observed that Runx2, a bone-specific transcription factor, is highly expressed myeloma major driver of progression bone. The primary goal present study was to identify Runx2-targeting miRNAs can reduce tumor growth. Expression analysis panel patient specimens, compared with healthy control revealed metastatic express low levels miR-342 miR-363 but high Runx2. Reconstituting (CAG)...

Inflammation-based prognostic scores, such as the glasgow score (GPS), index (PI), nutritional (PNI), neutrophil lymphocyte ratio (NLR) and platelet (PLR) were related to survival in many solid tumors. Recent study showed that GPS can be used predict outcome diffuse large B-cell lymphoma (DLBCL). However, other inflammation scores had not been reported it also remained unknown which of them was most useful evaluate DLBCLs. In this retrospective study, a number 252 newly diagnosed...

Increasing evidence suggests that the mononuclear/macrophage system is vital in amplifying inflammatory cascade IgA Nephropathy (IgAN). However, pathogenic mechanism of macrophages IgAN and targeted treatment strategies still need to be explored. This study found botanical triterpene celastrol (CLT) effectively alleviated renal lesions, M1-like macrophage infiltration, factors production, improved function mice. We patients had high expression ECM1, a crucial molecule involved polarization,...

The prognostic value of CD30 expression indiffuse large B-cell lymphoma (DLBCL)remains controversial. Herein, we performed this retrospective study to investigate the clinical and significance in patients with DLBCL.Among all 146 patients, was observed 23 cases (15.7%).The DLBCL showed more likely present B symptoms, bone marrow involvement, non-germinal centre B-cell-like (Non-GCB) DLBCL, BCL-2 Ki-67overexpression(p<0.05). Patients significantly poor overall event-free survivalcompared...

A major consequence of aging and stress, in yeast to humans, is an increased accumulation protein aggregates at distinct sites within the cells. Using genetic screens, immunoelectron microscopy, three-dimensional modeling our efforts elucidate importance aggregate annexation, we found that most accumulate near surface mitochondria. Further, show virus-like particles (VLPs), which are part retrotransposition cycle Ty elements, markedly enriched these aggregation. RNA interference-mediated...

Identification of de novo copy number variations (CNVs) across the genome in single cells requires single-cell whole-genome amplification and sequencing. Although many experimental protocols methods have been developed, all suffer from uneven distribution read depth after sequencing DNA amplicons, which constrains usage conventional CNV calling methodologies. Here, we present SCCNV, a software tool for detecting CNVs whole genome-amplified cells. SCCNV is read-depth based approach with...

ABSTRACT The six subunit ORC is essential for initiation of DNA replication in eukaryotes. Cancer cell-lines culture can survive and replicate after genetic inactivation individual subunits, ORC1, ORC2 or ORC5. In primary cells, ORC1 was dispensable the mouse liver endo-reduplication, but this could be explained by homolog, CDC6, substituting to restore functional ORC. Here, we have created mice with a conditional deletion ORC2, which does not homolog. Although embryo fibroblasts require...

The absolute lymphocyte count (ALC) has been reported to predict relapse in diffuse large B-cell lymphoma (DLBCL). We performed the present study determine whether ALC could identify preclinical during routine follow-up. Among all 148 patients analyzed, 39 exhibited relapse. Patients without had a higher compared with those proven at time of as well 1 and 3 months before Low (< 950/μL) positive predictive value 74.2% 69.0% negative 86.3% 84.0%, respectively, both was significantly associated...

Objective: To explore the prognostic value of international index (IPI), national comprehensive cancer network IPI(NCCN-IPI)and age-adjusted IPI (aa-IPI) in diffuse large B cell lymphoma. Methods: A total 311 patients with de novo B-cell lymphoma (DLBCL) diagnosed from 2003 to 2012 Nanfang hospital were included. All divided into CHOP (cyclophosphamide, vincristine, doxorubicin, and prednisone) R-CHOP (rituximab, CHOP) groups. Survival analysis was compared among IPI, NCCN-IPI aa-IPI models....

The recently updated "Guidelines for the Prevention and Treatment of Chronic Hepatitis B" in China have brought about significant changes. new treatment indications almost mandate implementation a Treat-all strategy chronically HBV-infected population China. While simultaneous negativity hepatitis B surface antigen (HBsAg) virus (HBV) DNA has long been an accepted criterion discontinuation, there controversies over initiation criteria starting with HBsAg HBV positivity. Despite inconsistent...

Somatic mutations accumulate in multiple organs and tissues during aging are a known cause of cancer. Cellular senescence is possible functional decline aging, yet also acts as an anticancer mechanism vivo. Here, we compared somatic mutation burden between early passage deeply senescent human fibroblasts using single-cell whole-genome sequencing. The results show that single-nucleotide variants (SNVs) small insertions deletions (INDELs) increased cells by about twofold but have the same...

Abstract Background Identification of de novo mutations from cell populations requires single-cell whole-genome sequencing (SCWGS). Although many experimental protocols SCWGS have been developed, few computational tools are available for downstream analysis different types somatic mutations, including copy number variation (CNV). Results We developed SCCNV, a software tool detecting CNVs whole genome-amplified single cells. SCCNV is read-depth based approach with adjustment the amplification...

The international staging system (ISS), based on serum beta-2 microglobulin and albumin, is used to predict survival in multiple myeloma, but its prognostic significance diffuse large B-cell lymphoma (DLBCL) remains unknown. Herein, we retrospectively analyzed 215 de novo DLBCL patients. According ISS, there were 90 of (41.9%) patients stage I, 98 (45.6%) II 27 (12.6%) III group. Patients with ISS II/III showed shorter overall (OS) event free (EFS) than those I treated R-CHOP (p = 0.012 p...

This paper proposes a new hybrid modulation mode (HMM) to eliminate the zero-crossing distortion of grid current and enable reactive power provision for H6 configuration PV (photovoltaic) grid-tied inverter. The common voltage, leakage current, efficiency proposed approach are also analyzed. In order improve frequency tracking novel self-adaptive proportional-integral-resonant (FSAPIR) controller is implemented which reduces error changes in frequency. provides basis accurately adjusting...