A5006266911- Fungal and yeast genetics research
- Gene Regulatory Network Analysis
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Heat shock proteins research
- Protein Structure and Dynamics
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Genetic Neurodegenerative Diseases
- Arsenic contamination and mitigation
- 3D Printing in Biomedical Research
- Muscle metabolism and nutrition
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- COVID-19 and Mental Health
- Biochemical and biochemical processes
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Extracellular vesicles in disease
- Plant Stress Responses and Tolerance
- DNA Repair Mechanisms
- Single-cell and spatial transcriptomics
- Parkinson's Disease Mechanisms and Treatments
Chalmers University of Technology
2022-2024
University of Gothenburg
2009-2024
Lasarettet i Motala
2023
Sahlgrenska University Hospital
2023
Arsenic is widely distributed in nature and all organisms possess regulatory mechanisms to evade toxicity acquire tolerance. Yet, little known about arsenic sensing signaling or their impact on tolerance detoxification systems. Here, we describe a novel role of the S. cerevisiae mitogen-activated protein kinase Hog1p protecting cells during exposure arsenite related metalloid antimonite. Cells impaired function are hypersensitive, whereas with elevated activity display improved...
Formation of amyloid-β (Aβ) fibrils is a central pathogenic feature Alzheimer's disease. Cell-secreted extracellular vesicles (EVs) have been suggested as disease modulators, although their exact roles and relations to Aβ pathology remain unclear. We combined kinetics assays biophysical analyses explore how small (<220 nm) EVs from neuronal non-neuronal human cell lines affected the aggregation disease-associated variant Aβ(1-42) into amyloid fibrils. Using thioflavin-T monitored seeding...
Spatial sorting to discrete quality control sites in the cell is a process harnessing toxicity of aberrant proteins. We show that yeast t-snare phosphoprotein syntaxin5 (Sed5) acts as key factor mitigating proteotoxicity and spatial deposition clearance IPOD (insoluble protein deposit) inclusions associates with disaggregase Hsp104. Sed5 phosphorylation promotes dynamic movement COPII-associated Hsp104 boosts disaggregation by favoring anterograde ER-to-Golgi trafficking. Hsp104-associated...
The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present application-specific microfluidic device, cellcomb, capable conducting high-throughput experiments. system employs pure hydrodynamic forces easy trapping is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques....
A major consequence of aging and stress, in yeast to humans, is an increased accumulation protein aggregates at distinct sites within the cells. Using genetic screens, immunoelectron microscopy, three-dimensional modeling our efforts elucidate importance aggregate annexation, we found that most accumulate near surface mitochondria. Further, show virus-like particles (VLPs), which are part retrotransposition cycle Ty elements, markedly enriched these aggregation. RNA interference-mediated...
The accumulation of misfolded proteins is a hallmark aging and many neurodegenerative diseases, making it important to understand how the cellular machinery recognizes processes such proteins. A key question in this respect whether are handled similar way regardless their genetic origin. To approach question, we compared three different proteins, guk1-7, gus1-3, pro3-1, by cell. We show that all nontoxic, even though highly overexpressed, highlighting usefulness analyzing response misfolding...
Aquaglyceroporins are transmembrane proteins belonging to the family of aquaporins, which facilitate passage specific uncharged solutes across membranes cells. The yeast aquaglyceroporin Fps1 is important for osmoadaptation by regulating intracellular glycerol levels during changes in external osmolarity. Upon high osmolarity conditions, accumulates increased production osmolyte and restricting efflux through Fps1. extended cytosolic termini contain short domains that flux channel. Here we...
Abstract The calcium-responsive phosphatase, calcineurin, senses changes in Ca 2+ concentrations a calmodulin-dependent manner. Here we report that under non-stress conditions, inactivation of calcineurin signaling or deleting the calcineurin-dependent transcription factor CRZ1 triggered formation chaperone Hsp100p (Hsp104p)-associated protein aggregates Saccharomyces cerevisiae . Furthermore, aggravated α-Synuclein-related cytotoxicity. Conversely, elevated production activator, Cnb1p,...
Central to proteopathies and leading most age-related neurodegenerative disorders is a failure in protein quality control (PQC). To harness the toxicity of misfolded damaged disease proteins, such proteins are either refolded, degraded by temporal PQC, or sequestered spatial PQC into specific, organelle-associated, compartments within cell. Here, we discuss impact vesicle trafficking pathways general, syntaxin 5 particular, as key players directing surface vacuole mitochondria, which...
The small heat shock protein Hsp42 and the t-SNARE Sed5 have central roles in sequestration of misfolded proteins into insoluble deposits yeast Saccharomyces cerevisiae. However, whether these proteins/processes interact quality control (PQC) is not known. Here, we show that anterograde trafficking modulate phosphorylation partially via MAPK kinase Hog1. Such phosphorylation, specifically at residue S215, abrogated co-localization with Hsp104 disaggregase, aggregate clearance, chaperone...
Background Neurological manifestations in patients with COVID-19 have been reported previously as outcomes of the infection. The purpose current study was to investigate occurrence neurological signs and symptoms patients, county Östergötland southeastern Sweden. Methods This is a retrospective, observational cohort study. Data were collected between March 2020 June 2020. Information extracted from medical records by trained research assistant physician all data validated senior neurologist....
Arsenic is a highly toxic and carcinogenic metalloid widely dispersed in the environment, contaminating water soil accumulating crops. Paradoxically, arsenic also part of modern therapy employed treating numerous ailments diseases. Hence, inventing strategies to tune cellular uptake based on purpose striking. Here, we describe an approach which arsenite can be increased using MAPK inhibitor. Employing microfluidic flow chambers combination with optical tweezers fluorescent microscopy,...
Single cell analysis techniques provide a unique opportunity of determining the intercellular heterogeneity in population, which due to genotype variations and different physiological states cells i.e. size, shape age, cannot be retrieved from averaged population values. In order obtain high-value quantitative data single-cell experiments it is important have experimental platforms enabling high-throughput studies. Here, we present microfluidic chip, capable capturing individual suspension...
Regulating arsenic uptake is imperative due to its carcinogenicity. Combining microfluidics, optical tweezers and fluorescence microscopy, the arsenite by Fps1 using a selective kinase inhibitor investigated single cell analysis platform.