A5013793955- Arsenic contamination and mitigation
- Fungal and yeast genetics research
- Herpesvirus Infections and Treatments
- Trace Elements in Health
- RNA regulation and disease
- DNA Repair Mechanisms
- Cytomegalovirus and herpesvirus research
- Genomics and Chromatin Dynamics
- Animal Nutrition and Physiology
- Redox biology and oxidative stress
- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Plant Stress Responses and Tolerance
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Phytase and its Applications
- Molecular Biology Techniques and Applications
- Enzyme Production and Characterization
- Phagocytosis and Immune Regulation
- Insect Resistance and Genetics
- Aluminum toxicity and tolerance in plants and animals
- Protein Structure and Dynamics
- Nuclear Issues and Defense
Tufts University
2020-2022
Novozymes (Denmark)
2018-2022
Northwestern University
2020
DuPont (Denmark)
2013-2015
University of Copenhagen
2009-2013
University of Gothenburg
2004-2012
Carlsberg Laboratory
2007-2008
Abstract Background Arsenic is a toxic and highly abundant metalloid that endangers human health through drinking water the food chain. The most common forms of arsenic in environment are arsenate (As(V)) arsenite (As(III)). As(V) non-functional phosphate analog enters chain via plant transporters. Inside cells, becomes reduced to As(III) for subsequent extrusion or compartmentation. Although much known about transport handling microbes mammals, systems have not yet been characterized...
Arsenic is widely distributed in nature and all organisms possess regulatory mechanisms to evade toxicity acquire tolerance. Yet, little known about arsenic sensing signaling or their impact on tolerance detoxification systems. Here, we describe a novel role of the S. cerevisiae mitogen-activated protein kinase Hog1p protecting cells during exposure arsenite related metalloid antimonite. Cells impaired function are hypersensitive, whereas with elevated activity display improved...
Arsenic is ubiquitously present in nature, and various mechanisms have evolved enabling cells to evade toxicity acquire tolerance. Herein, we explored how Saccharomyces cerevisiae (budding yeast) respond trivalent arsenic (arsenite) by quantitative transcriptome, proteome, sulfur metabolite profiling. Arsenite exposure affected transcription of genes encoding functions related protein biosynthesis, detoxification, oxidative stress defense, redox maintenance, proteolytic activity....
Abstract Background Arsenic and cadmium are widely distributed in nature pose serious threats to the environment human health. Exposure these nonessential toxic metals may result a variety of diseases including cancer. However, arsenic toxicity targets cellular systems contributing tolerance acquisition not fully known. Results To gain insight into metal action mechanisms, we carried out genome-wide screening Saccharomyces cerevisiae haploid homozygous diploid deletion mutant collections...
All organisms are equipped with systems for detoxification of the metalloids arsenic and antimony. Here, we show that two parallel pathways involving AP-1-like proteins Yap1p Yap8p required acquisition metalloid tolerance in budding yeast S. cerevisiae. is demonstrated to reside nucleus where it mediates enhanced expression genes ACR2 ACR3. Using chromatin immunoprecipitation assays, associated ACR3 promoter untreated as well arsenic-exposed cells. Like Yap1p, specific cysteine residues...
Transcription factors govern gene expression by binding to short DNA sequences called cis-regulatory elements. These are typically located in promoters, which regions of variable length upstream the open reading frames genes. Here, we report that promoter and function related yeast, fungi, plants. In particular, promoters for stress-responsive genes general longer than those other Essential have, on hand, relatively promoters. We utilize these findings a novel method identifying relevant...
Arsenic is an environmental toxin and a worldwide health hazard. Since this metalloid ubiquitous in nature, virtually all living organisms require systems for detoxification tolerance acquisition. Here, we show that during chronic exposure to arsenite [As(III)], Saccharomyces cerevisiae (budding yeast) exports accumulates the low-molecular-weight thiol molecule glutathione (GSH) outside of cells. Extracellular accumulation triglutathione complex As(GS)₃ was also detected direct transport...
Herpesviruses are large viruses that infect nearly all vertebrates and some invertebrates cause lifelong infections in most of the world’s population. During replication, herpesviruses export their capsids from nucleus into cytoplasm by an unusual mechanism which viral nuclear egress complex (NEC) deforms membrane around capsid.
Saccharomyces cerevisiae uses several mechanisms for arsenic detoxification including the arsenate reductase Acr2p and arsenite efflux protein Acr3p. ACR2 ACR3 are transcribed in opposite directions from same promoter expression of these genes is regulated by AP-1 (activator 1)-like transcription factor Yap8p. Yap8p has been shown to permanently associate with this stimulate ACR2/ACR3 response arsenic. In present study we characterized DNA sequence that targeted We show binds a...
The efficacy and safety of a novel Subtilisin protease from
Physical stability during storage and against processing such as dehyration/rehydration are the cornerstone in designing delivery vehicles. In this work, mono-, di- tri-saccharides were enzymatically conjugated to phosphatidyl group through a facile approach namely phospholipase D (PLD) mediated transphosphatidylation biphasic reaction system. The purified products structurally identified connectivities of carbohydrate moiety precisely mapped by 1H, 31P, 13C NMR pulse sequences LC-ESI-FTMS....
Abstract Background In fission yeast, centromeric heterochromatin is necessary for the fidelity of chromosome segregation. Propagation in dividing cells requires RNA interference (RNAi) and transcription repeats by polymerase II during S phase cell cycle. Results We found that Med8-Med18-Med20 submodule Mediator complex required transcriptional regulation native dh dg silencing reporter genes inserted heterochromatin. Mutations did not alter occupancy at centromeres; however, they led to an...
In eukaryotes many players in the DNA-damage response (DDR) catalyze protein sumoylation or ubiquitylation. Emphasis has been placed on how these modifications orchestrate sequential recruitment of repair factors to sites DNA damage stalled replication forks. Here, we shed light a pathway which sumoylated are eliminated through coupled action Sumo-targeted ubiquitin ligases (STUbLs) and ubiquitin-fusion degradation 1 (Ufd1). Ufd1 is subunit Cdc48-Ufd1-Npl4 complex implicated sorting...
During replication, herpesviral capsids are translocated from the nucleus into cytoplasm by an unusual mechanism, termed nuclear egress, that involves capsid budding at inner membrane. This process is mediated viral egress complex (NEC) deforms membrane around capsid. Although NEC essential for across all three subfamilies of Herpesviridae , most studies to date have focused on homologs alpha- and beta- but not gammaherpesviruses. Here, we report crystal structure Epstein-Barr virus (EBV), a...
Dipyridyl disulfide (DPS) is a highly reactive thiol oxidant that functions as electron acceptor in thiol-disulfide exchange reactions. DPS very toxic to yeasts, impairing growth at low micromolar concentrations. The genes TRX2 (thioredoxin), SOD1 (superoxide dismutase), GSH1 (gamma-glutamyl-cysteine synthetase) and, particularly, GLR1 (glutathione reductase) are required for survival on DPS. uniquely thiol-specific, and we found the cellular mechanisms detoxification differ substantially...
Enzymes can aid in optimal feed stock utilization when used as additives. A range of toxicological studies were performed to evaluate the safety profile a novel phytase (phytase HM) from Citrobacter braakii produced Aspergillus oryzae. Phytase HM was found be non-mutagenic and non-clastogenic vitro tests. Further, preparation did not exhibit irritative potential eye skin applied models. 13-week subchronic toxicity study with oral administration rats show any adverse effects. Efficacy showed...
// Klaus Højgaard Jensen 1, 2, * , Jose M.G. Izarzugaza Agnieszka Sierakowska Juncker Rasmus Borup Hansen 2 Torben Frøstrup 3 Pascal Timshel 1 Thorarinn Blondal 4 Thomas Skøt Eske Rygaard-Hjalsted Peter Mouritzen Michael Thorsen Wernersson Henrik Bjørn Nielsen Anders Jakobsen 3, Søren Brunak 5, and Flemming Brandt Sørensen 6, Department of Bio Health Informatics, Technical University Denmark, Kgs, Lyngby 2800, Denmark Intomics A/S, Oncology Department, Vejle Hospital, 7100, Exiqon Vedbaek...
The current study presents a safety evaluation of novel glucuronoxylan hydrolase (EC 3.2.1.136) from Bacillus subtilis produced in licheniformis. preparation did not exhibit irritative potential to the eye and skin when applied vitro models. was non-mutagenic non-clastogenic tests. Oral administration rats cause any adverse effect 90-days subchronic toxicity study. A tolerance performed with broiler chickens confirmed that this is safe for fed at maximum recommended dose, as well 10 times...
A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have characterized. Here we show that rsc nhp6ΔΔ mutants, deficient for the function of remodeling factor RSC architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at restrictive temperature. In addition, demonstrate rsc8-ts16 cells contain low levels U6 snRNA U4/U6 di-snRNA is further exacerbated after two hours growth This...