A5020371384- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Fermentation and Sensory Analysis
- Genomics, phytochemicals, and oxidative stress
- Mitochondrial Function and Pathology
- Plant Molecular Biology Research
- Biochemical Analysis and Sensing Techniques
- Light effects on plants
- Photoacoustic and Ultrasonic Imaging
- GABA and Rice Research
- Postharvest Quality and Shelf Life Management
- Microbial Metabolic Engineering and Bioproduction
- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- ATP Synthase and ATPases Research
- Genetic Neurodegenerative Diseases
- Gene Regulatory Network Analysis
- Pluripotent Stem Cells Research
- Endoplasmic Reticulum Stress and Disease
- Advanced Fluorescence Microscopy Techniques
- Advanced Biosensing Techniques and Applications
Instituto de Biología Molecular y Celular de Plantas
2013-2023
Consejo Superior de Investigaciones Científicas
2021-2023
Universitat Politècnica de València
2013-2023
Universitat de València
2023
University of Amsterdam
2017-2020
The conserved protein kinase Sch9 is a central player in the nutrient-induced signaling network yeast, although only few of its direct substrates are known. We now provide evidence that controls vacuolar proton pump (V-ATPase) to maintain cellular pH homeostasis and ageing. A synthetic sick phenotype arises when deletion SCH9 combined with dysfunctional V-ATPase, lack has significant impact on cytosolic (pHc) homeostasis. physically interacts with, influences glucose-dependent...
Spatial sorting to discrete quality control sites in the cell is a process harnessing toxicity of aberrant proteins. We show that yeast t-snare phosphoprotein syntaxin5 (Sed5) acts as key factor mitigating proteotoxicity and spatial deposition clearance IPOD (insoluble protein deposit) inclusions associates with disaggregase Hsp104. Sed5 phosphorylation promotes dynamic movement COPII-associated Hsp104 boosts disaggregation by favoring anterograde ER-to-Golgi trafficking. Hsp104-associated...
Abstract Background Salt stress is one of the main constraints determining crop productivity, and therefore limitations for food production. The aim this study was to characterize salt response at physiological molecular level different Broccoli ( Brassica oleracea L. var. Italica Plenck) cultivars that were previously characterized in field greenhouse trials as sensitive or tolerant. This aimed identify functional traits capable predicting ability uncharacterized lines cope with stress. For...
Melon (Cucumis melo L.) is a crop with important agronomic interest worldwide. Because of the increase drought and salinity in many cultivation areas as result anthropogenic global warming, obtention varieties tolerant to these conditions major objective for agronomical improvement. The identification limiting factors stress tolerance could help define objectives traits which be improved by classical breeding or other techniques. With this objective, we have characterized, at physiological...
Fine-tuned activation of gene expression in response to stress is the result dynamic interactions transcription factors with specific promoter binding sites. In study described here we used a time-resolved luciferase reporter assay living Saccharomyces cerevisiae yeast cells gain insights into how osmotic and oxidative signals modulate dose-sensitive manner. Specifically, dose-response behavior four different natural promoters (GRE2, CTT1, SOD2, CCP1) reveals differences their sensitivity...
Broccoli is a cruciferous crop rich in health-promoting metabolites. Due to several factors, including anthropogenic global warming, aridity increasing many cultivation areas. There great demand characterize the drought response of broccoli and use this knowledge develop new cultivars able maintain yield under water constraints. The aim study at physiological molecular level different (Brassica oleracea L. var. Italica Plenck) cultivars, previously characterized as drought-sensitive or...
Yeast biomass is recycled in the process of bioethanol production using treatment with dilute sulphuric acid to control bacterial population. This can lead loss cell viability, consequences on fermentation yield. Thus, aim this study was define functional cellular responses inorganic stress. Saccharomyces cerevisiae strains mutation several signalling pathways, as well cells expressing pH-sensitive GFP derivative ratiometric pHluorin, were tested for survival and cytosolic pH (pHc) variation...
Broccoli (Brassica oleracea L. var. Italica Plenck) is a cruciferous crop that considered to be good source of micronutrients. Better taste main objective for breeding, as consumers are demanding novel cultivars suited healthy diet, but ones more palatable. This study aimed identify primary metabolites related with better according consumer panel. For this purpose, we performed complete metabolomic profile 20 different broccoli grown in the field and contrasted obtained data results panel...
Summary The new high‐sensitive and high‐resolution technique, Re‐scan Confocal Microscopy (RCM), is based on a standard confocal microscope extended with re‐scan detection unit. unit includes pair of re‐scanning mirrors that project the emission light onto camera in scanning manner. signal‐to‐noise ratio improved by factor 4 compared to microscopy lateral resolution 170 nm (compared 240 for diffraction limited resolution, 488 excitation, 1.49 NA). Apart from sensitivity optical setup...
Abstract Calorie restriction is the only physiological intervention that extends lifespan throughout all kingdoms of life. In budding yeast Saccharomyces cerevisiae, cytosolic pH (pH c ) controls growth and responds to nutrient availability, decreasing upon glucose depletion. We investigated interactions between central signalling cAMP‐Protein Kinase A (PKA) pathway. Glucose abundance during phase enhanced acidification depletion, via modulation PKA activity. This actively controlled...
The protein kinase C (PKC) family plays important regulatory roles in numerous cellular processes. Saccharomyces cerevisiae contains a single PKC, Pkc1, whereas mammals, the PKC comprises nine isoforms. Both Pkc1 and novel isoform PKCδ are involved control of DNA integrity checkpoint activation, demonstrating that this mechanism is conserved from yeast to mammals. To explore function non-tumor cell line, we employed CRISPR-Cas9 technology obtain knocked-out mouse embryonic stem cells...