A5064600858- Connexins and lens biology
- Heat shock proteins research
- Immunotherapy and Immune Responses
- Endoplasmic Reticulum Stress and Disease
- Gestational Trophoblastic Disease Studies
- Yersinia bacterium, plague, ectoparasites research
- Biochemical effects in animals
- Ophthalmology and Visual Impairment Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Cellular transport and secretion
- Prion Diseases and Protein Misfolding
- Natural product bioactivities and synthesis
- Inflammatory mediators and NSAID effects
- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- Neuroendocrine Tumor Research Advances
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Alzheimer's disease research and treatments
- Glaucoma and retinal disorders
- Radiation Therapy and Dosimetry
- Effects of Radiation Exposure
- Aldose Reductase and Taurine
- RNA Research and Splicing
Durham University
2017-2023
Public Health England
2021
KU Leuven
2017
National Children’s Research Centre
2014
Children's Health Ireland at Crumlin
2014
The conserved protein kinase Sch9 is a central player in the nutrient-induced signaling network yeast, although only few of its direct substrates are known. We now provide evidence that controls vacuolar proton pump (V-ATPase) to maintain cellular pH homeostasis and ageing. A synthetic sick phenotype arises when deletion SCH9 combined with dysfunctional V-ATPase, lack has significant impact on cytosolic (pHc) homeostasis. physically interacts with, influences glucose-dependent...
BFSP1 (beaded filament structural protein 1, filensin) is a cytoskeletal expressed in the eye lens. It binds AQP0 vitro and its C-terminal sequences have been suggested to regulate water channel activity of AQP0. A myristoylated fragment from C-terminus was found enriched fractions. Here we identify as substrate for caspase-mediated cleavage at several sites including D433. Cleavage D433 exposes cryptic myristoylation sequence (434–440). We confirm that this an excellent both NMT1 2...
Organ and tissue development are highly coordinated processes; lens growth functional integration into the eye (emmetropia) is a robust example. An epithelial monolayer covers anterior hemisphere of lens, its organization key to formation optical properties throughout all life stages. To better understand how epithelium supports function, we have developed novel whole imaging system using conventional confocal light microscopy specialized analysis software produce three-dimensional maps for...
Recent studies apparently finding deleterious effects of radiation exposure on cataract formation in birds and voles living near Chernobyl represent a major challenge to current protection regulations. This study conducted an integrated assessment cataractogenesis using the most advanced technologies available assess status lenses extracted from fish caught at both Ukraine Fukushima Japan. It was hypothesised that these novel data would reveal positive correlations between dose early...
Lens epithelial cell proliferation and differentiation are naturally well regulated controlled, a characteristic essential for lens structure, symmetry function. The effect of ionizing radiation on has been demonstrated in previous studies at high acute doses, but the dose rate not yet considered. In this work, mice received single doses 0.5, 1 2 Gy radiation, rates 0.063 0.3 Gy/min. Eye lenses were isolated postirradiation 30 min up until 14 days flat-mounted. Then, determined using...
BFSP1 (beaded filament structural protein 1) is a plasma membrane, Aquaporin 0 (AQP0/MIP)-associated intermediate expressed in the eye lens. myristoylated, post-translation modification that requires caspase cleavage at D433. Bioinformatic analyses suggested sequences 434-452 were α-helical and amphipathic.
Ionising radiation (IR) is a cause of lipid peroxidation, and epidemiological data have revealed correlation between exposure to IR the development eye lens cataracts. Cataracts remain leading blindness around world. The plasma membranes fibre cells are one most cholesterolrich in human body, forming rafts contributing biophysical properties membrane. Liquid chromatography followed by mass spectrometry was used analyse bovine membrane fractions after 5 50 Gy lenses taken from wholebody 2...
The eye lens is not only a radiosensitive tissue, but also one where the response to ionising radiation (IR) cell location specific. Moreover this behaviour affects emergent properties of cells in epithelium causing deregulation normally geometrically constrained epithelial as that transition from germinative zone meridional rows at very periphery lens. Epithelial are, however, much slower than either central or isolated blood lymphocytes same animals their repair double strand breaks...
Abstract Epidemiological data correlates exposure to low dose ionising radiation (IR) and cataractogenesis later in life. Our understanding of the underlying biological mechanisms is still its early stages as we seek explore applicability linear no‐threshold model these rate exposures. A single layer epithelial cells (LECs) covers anterior hemisphere lens. Central peripheral regions (PR) are designated on basis position cell density. The PR considered most radiosensitive region epithelium....
Purpose To investigate changes in both lens shape and the epithelial distribution as emmetropia is established C57Bl6J mice between postnatal weeks 4 to 6. Methods We used a computational approach produce geometrical maps of mouse its epithelium. Conveniently our ulitises data collected from intact lenses imaged using conventional laser scanning confocal microscope. It delivers cartesian coordinates for every cell chose during 6, time development when this inbred strain (Tkatchenko et al .,...