A5080968919- Connexins and lens biology
- Ion Transport and Channel Regulation
- Aldose Reductase and Taurine
- Fish Ecology and Management Studies
- Intraocular Surgery and Lenses
- Spectroscopy and Quantum Chemical Studies
- Surfactants and Colloidal Systems
- Photoreceptor and optogenetics research
- Optical Polarization and Ellipsometry
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Biochemical effects in animals
- Retinal Development and Disorders
- Congenital heart defects research
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Ubiquitin and proteasome pathways
- Ion channel regulation and function
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Ion Channels and Receptors
- Tribology and Lubrication Engineering
- Peptidase Inhibition and Analysis
- Advanced X-ray Imaging Techniques
- Heat shock proteins research
University of California, Irvine
2016-2024
University of Auckland
2014-2024
Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular and water dynamics are needed to understand their roles in physiology fate determination. the lens essential normal optics, an understanding its regulation will help prevent cataract presbyopia. Here, we combine use nanoenvironmental sensor [6-acetyl-2-dimethylaminonaphthalene (ACDAN)] visualize with aquaporin 0 zebrafish mutants that disrupt regulation. Spectral phasor analysis ACDAN fluorescence...
To investigate the roles of Aquaporin 0a (Aqp0a) and Aqp0b in zebrafish lens development transparency.CRISPR/Cas9 gene editing was used to generate loss-of-function deletions aqp0a and/or aqp0b. Wild type (WT), single mutant, double mutant lenses were analyzed from embryonic adult stages. Lens transparency, morphology, growth assessed. Immunohistochemistry map protein localization as well assess tissue organization distribution cell nuclei.aqp0a-/- aqp0b-/- cause cataracts with variable...
The optics of the eye is key to a functioning visual system. exact nature correlation between ocular and development not known because paucity knowledge about growth optical element, lens. sophisticated lens its gradient refractive index provide superior quality that needs which, it thought, has major influence on proper function. lens, however, renders accurate measurements difficult make been reason why function remains largely unknown. Novel imaging techniques have made possible...
KRas-induced actin-interacting protein (KRAP) has been identified as crucial for the appropriate localization and functioning of inositol trisphosphate receptors (IP3Rs) that mediate Ca2+ release from endoplasmic reticulum. Here, we used siRNA knockdown KRAP expression in HeLa HEK293 cells to examine roles generation IP3-mediated local puffs global, cell-wide signals. High resolution imaging revealed mean amplitude was strongly reduced by knockdown, whereas flux during openings individual...
BFSP1 (beaded filament structural protein 1, filensin) is a cytoskeletal expressed in the eye lens. It binds AQP0 vitro and its C-terminal sequences have been suggested to regulate water channel activity of AQP0. A myristoylated fragment from C-terminus was found enriched fractions. Here we identify as substrate for caspase-mediated cleavage at several sites including D433. Cleavage D433 exposes cryptic myristoylation sequence (434–440). We confirm that this an excellent both NMT1 2...
Purpose: In the eye lens, cytosolic protein concentrations increase progressively from periphery to center, contributing gradient of refractive index (GRIN). Aquaporins are membrane proteins lens fiber cells that regulate water transport and adhesion interact with cytoskeletal proteins. This study investigates how these contribute proper development GRIN. Methods: Loss-of-function deletions aqp0a and/or aqp0b in zebrafish were generated using CRISPR/Cas9 gene editing. Lenses single aqp0a−/−...
Purpose.: To identify whether the kinases that regulate activity of cation chloride cotransporters (CCC) in other tissues are also expressed rat and human lenses. Methods.: The expression with-no-lysine kinase (WNK 1, 3, 4), oxidative stress response 1 (OSR1), Ste20-like proline alanine rich (SPAK) were determined at either transcript or protein levels lenses by reverse-transcriptase PCR and/or Western blotting, respectively. Selected regionally subcellularly characterized transparency, wet...
Aquaporin 0 (AQP0) is the most abundant lens membrane protein, and loss of function in human animal models leads to cataract formation. AQP0 has several functions including water transport adhesion. Since optics rely on strict tissue architecture achieved by compact cell-to-cell adhesion between fiber cells, understanding how contributes would shed light normal physiology pathophysiology. We show an vitro assay that one two closely related zebrafish Aqp0s, Aqp0b, strong auto-adhesive...
The zebrafish is uniquely suited to genetic manipulation and in vivo imaging, making it an increasingly popular model for reverse studies generation of transgenics imaging. These unique capabilities make the ideal platform study ocular lens development physiology. Our recent findings that Aquaporin-0, Aqp0a, required stability anterior suture, as well shift nucleus center with age led us develop tools especially analyzing properties lenses. Here we outline detailed methods dissection can be...
The zebrafish is uniquely suited to genetic manipulation and in vivo imaging, making it an increasingly popular model for reverse studies generation of transgenics imaging. These unique capabilities make the ideal platform study ocular lens development physiology. Our recent findings that Aquaporin-0, Aqp0a, required stability anterior suture, as well shift nucleus center with age led us develop tools especially analyzing properties lenses. Here we outline detailed methods dissection can be...
Optical development of the zebrafish eye relies on movement highly refractive lens nucleus from an anterior to a central location in optical axis during development. We have shown that this mechanism turn depends function Aquaporin 0a (Aqp0a), multifunctional and extremely abundant protein fiber cell membranes. Here, we probe specific cellular functions necessary for rescuing centralization defects aqp0a-/- null mutants by stable overexpression Aqp0 orthologue killifish, MIPfun.
Abstract Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular and ultimately water-dynamics are needed to understand their role in fates. The the lens essential understanding normal optics lens, moreover prevention cataract presbyopia. Here we combine use water nano-environmentally sensitive sensor (6-acetyl-2-dimethylaminonaphthalene, ACDAN) with Aquaporin zero zebrafish mutants crowding. Spectral phasor analysis ACDAN fluorescence reveal extent...