A5054296045- Connexins and lens biology
- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- Advanced Drug Delivery Systems
- Advanced Glycation End Products research
- Advancements in Transdermal Drug Delivery
- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Adsorption and biosorption for pollutant removal
- Intraocular Surgery and Lenses
- Biochemical effects in animals
- Pharmacy and Medical Practices
- Drug Solubulity and Delivery Systems
- Dermatology and Skin Diseases
- Retinal Development and Disorders
- Ocular Diseases and Behçet’s Syndrome
- Phosphorus and nutrient management
- Corneal surgery and disorders
- Heat shock proteins research
- Silk-based biomaterials and applications
- Analytical Methods in Pharmaceuticals
- Chemical Synthesis and Characterization
- Retinal and Optic Conditions
- Pharmacological Effects of Natural Compounds
- Vanadium and Halogenation Chemistry
Kindai University
2016-2025
Osaka Police Hospital
2020
University of Miyazaki
2007-2019
Yao Tokushukai General Hospital
2015
Higashi Osaka City General Hospital
2008
Hyogo Medical University
2007
Engineering Advancement Association of Japan
2002
Saiseikai Kanagawa Hospital
2001
Mitsui (Japan)
1990
Activation of BCR::ABL1 tyrosine kinase is the main pathogenic mechanism underlying chronic myeloid leukemia (CML) in 90% affected patients. The prognosis for individuals with CML who receive treatment inhibitors (TKIs) such as imatinib, promising, a 5-year survival rate >90%. However, unfortunately, 20-30% patients are treated imatinib may become resistant to TKIs. objective present study was determine whether E3 ubiquitin-protein ligase Mdm2 (MDM2), regulator p53 that promotes apoptosis...
We previously found that ophthalmic formulations containing nanoparticles prepared by a bead mill method lead to an increase in bioavailability comparison with traditional (solution type). However, the transcorneal penetration pathway for has not been explained yet. In this study, we investigated mechanism of application indomethacin (IMC-NPs).IMC-NPs was method. For analysis energy-dependent endocytosis, corneal epithelial (HCE-T) cell monolayers and removed rabbit cornea were...
We designed formulations based on minoxidil (MXD) nanoparticles (N-MXD) and examined whether N-MXD can increase drug delivery into the follicles. In addition, we investigated effect of hair growth in C57BL/6 mice.N-MXD (1%) was prepared as follows: methylcellulose, p-hydroxyalkylbenzoates, mannitol, MXD were dispersed purified water milled using zirconia beads under refrigeration (5500 rpm, 30 s×15 times, intermittent milling). mice used to evaluate hair-growth effects. The expression levels...
The protein sericin is the main constituent of silk. We demonstrate effects on corneal wound healing in rat debrided epithelium. also determined cell adhesion and proliferation a human cornea epithelial line (HCE-T). Epithelium was removed from corneas rats with BD Micro-Sharp, wounded were dyed 1% fluorescein solution. wounds monitored using fundus camera TRC-50X equipped digital camera. instilled saline approximately 10% at 12 h, 65% 24 h after abrasion. showed almost complete by 36 On...
We prepared ophthalmic formulations containing 0.5% tranilast (TL) nanoparticles using 0.005% benzalkonium chloride (BAC), D-mannitol, and 2-hydroxypropyl-β-cyclodextrin (HPβCD), investigated their usefulness in the ophthalmologic field by evaluating corneal toxicity permeability. TL were zirconia beads Bead Smash 12, which allowed preparation of high quality dispersions (particle size, 34 ± 20 nm, means S.D.). Dispersions are tolerated better human epithelium cells than a commercially...
In the clinical setting, raloxifene, a second-generation selective estrogen receptor modulator, is administered orally; however, bioavailability (BA) only 2% because of its poor solubility in aqueous fluids and extensive first-pass metabolism. Therefore, it expected that development transdermally delivered formulation may reduce necessary dose without compromising therapeutic efficacy. this study, we designed transdermal formulations containing raloxifene nanoparticles evaluated their...
Indomethacin (IMC), a nonsteroidal anti-inflammatory drug, has been used in the treatment of rheumatoid arthritis (RA), although its clinical use limited by systemic side effects that include gastrointestinal lesions. Therefore, development IMC formulations do not cause lesions is highly anticipated. In this study, we designed new topical containing solid nanoparticles (IMCnano gel ointment), and investigated their pharmacokinetics. addition, demonstrate preventive application on...
Effective therapies have yet to be established for pancreatic ductal adenocarcinomas (PDAC) even though it is the most aggressive cancer. In present study, PDAC was analyzed using novel rat mAbs against membrane proteins in conjunction with flow cytometry and immunohistochemistry. Human epidermal growth receptor (HER)1–4, mesenchymal epithelial transition factor (MET), sphingosine‐1‐phospahate 1 (S1PR1), l ‐type amino acid transporter (LAT1), system x − c (xCT), alanine‐serine‐cysteine...
This study investigated the anti-inflammatory effects of water-dispersible hesperetin (WD-Hpt) in an endotoxin-induced uveitis (EIU) rat model. The rats were orally administered 10, 25, or 50 mg/kg WD-Hpt immediately after lipopolysaccharide (LPS) injection at concentration 200 μg. Clinical scores, cellular inflammation, aqueous humor (ApH) protein concentration, as well levels tumor necrosis factor (TNF)-α, cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) AqH, histopathological...
Abstract Background Chronic myeloid leukemia is associated with a more favorable prognosis following treatment BCR::ABL1 tyrosine kinase inhibitors (TKIs). Nonetheless, about 40% of affected individuals CML display resistance or intolerance towards TKIs. Heat shock protein 90 (HSP90) functions as molecular chaperone and known for its overexpression in various types cancer, thereby HSP90 potential candidate the TKI-resistant -sensitive CML. In present study, we aimed to investigate whether...
Developmental toxicity testing is essential to identify substances that may harm embryonic development. This study aimed establish a protocol for evaluating developmental using human induced pluripotent stem cells (iPSCs) by analyzing cellular activity and gene expression changes. Two ICH S5(R3) positive substances, valproic acid (VPA), which substance previously detected as other test methods, thalidomide (Thalido), were examined during early trichoderm differentiation without fetal bovine...
Background and Objectives: In previous studies, we reported that the assessment of cumulative thermal dose in crystalline lens, conducted through computational modeling utilizing a supercomputer biothermal transport equation, exhibited significant association with incidence nuclear cataracts. this study, have investigated types proteins expressed underlying 35.0 °C (normal-temp) 37.5 (warming-temp) by using shotgun liquid chromatography (LC) tandem mass spectrometry (MS/MS)-based global...
In the present study, magnetic-calcined bamboo composite adsorbents (MCBC200, MCBC400, MCBC600, MCBC800, and MCBC1000) were prepared, their physicochemical characteristics (scanning electron microscope images, differential thermogravimetric analysis, Fourier transform-IR, specific surface area, functional groups, point of zero charge [pHpzc]) evaluated. Furthermore, adsorption capacity methylene blue (MB, cationic dye) using prepared was assessed. The value pHpzc area MCBC400 7.8 50.6 m2/g,...
We previously reported that carbopol hydrogels incorporating indomethacin nanoparticles (IMC NPs) improved the low permeability and bioavailability of skin formulations in transdermal drug delivery systems. However, combination NPs with other types has not been sufficiently explored to date. Therefore, this study investigated propylene glycol (PG)/polyvinyl alcohol (PVA) hydrogel as an alternative base for IMC NPs. were prepared using bead milling treatment, these incorporated into PG/PVA...
This study examined the potential of waste basil seeds (BSs) calcined at 500°C or 1000°C (BS500 BS1000, respectively) for gadolinium removal from aqueous solutions. Gadolinium ion adsorption onto produced adsorbents was also assessed in relation to a number parameters, including initial concentration, temperature, exposure time, and pH. Higher concentrations, temperatures, times (BS, BS500 ≒ BS1000) resulted an increase quantity adsorbed ions; To further understand mechanism, detailed...
The protein sericin is the main constituent of silk. We investigated effects on corneal wound healing in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model for human type 2 diabetes. Corneal wounds were prepared by removal epithelium, and documented using TRC-50X equipped with digital camera. Sericin solutions instilled into eyes rats five times day following abrasion. Plasma glucose triglycerides determined an Accutrend GCT. Cholesterol insulin measured E-Test Kit ELISA Insulin Kit,...
Tranilast (TL), an antiallergic agent, has been clinically used in the treatment of bronchial asthma, although its clinical use limited by poor solubility water, photodegradation and systemic side effects. In this study, we prepared a gel ointment containing TL nanoparticles (TLnano ointment), investigated usefulness. addition, demonstrated preventive effects TLnano on inflammation adjuvant-induced arthritis (AA) rats. The was using Bead Smash 12 (a bead mill) additives including sodium...
It was reported that cilostazol (CLZ) suppressed disruption of the microvasculature in ischemic areas. In this study, we have designed novel injection formulations containing CLZ nanoparticles using 0.5% methylcellulose, 0.2% docusate sodium salt, and mill methods (CLZnano dispersion; particle size 81 ± 59 nm, mean S.D.), investigated their toxicity usefulness a cerebral ischemia/reperfusion-induced injury model (MCAO/reperfusion mice). The pharmacokinetics injections CLZnano dispersions is...