This study investigated the anti-inflammatory effects of water-dispersible hesperetin (WD-Hpt) in an endotoxin-induced uveitis (EIU) rat model. The rats were orally administered 10, 25, or 50 mg/kg WD-Hpt immediately after lipopolysaccharide (LPS) injection at concentration 200 μg. Clinical scores, cellular inflammation, aqueous humor (ApH) protein concentration, as well levels tumor necrosis factor (TNF)-α, cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) AqH, histopathological...

A single suction microelectrode voltage-clamp technique was used to study the actions of lanthanum ions (La3+) on ionic currents in cells isolated from bullfrog right atrium. La3+, added as LaCl3, blocked "slow" inward Ca2+ current (ICa) a dose-dependent fashion; 10(-5) M produced complete inhibition. This effect best fitted by dose-response curve that calculated assuming 1:1 binding La3+ site having dissociation constant 7.5 x 10(-7) M. block reversed (to 90% control ICa) following washout...

Individual myocytes were isolated from bullfrog atrium by enzymatic and mechanical dispersion, a one-microelectrode voltage clamp was used to record the slow outward K+ currents. In normal [K+]o (2.5 mM), current tails reverse between -95 -100 mV. This finding, observed 51-mV shift of Erev/10-fold change in [K+]o, strongly suggest that "delayed rectifier" atrial cells is current. current, IK, plays an important role initiating repolarization, it distinct quasi-instantaneous, inwardly...

The present study aimed to investigate the therapeutic efficacy of post-inflammatory treatment with luteolin on endotoxin-induced uveitis (EIU) in rats. Intraperitoneal injection 10 mg/kg or 1 prednisolone (Pred) at 4 hr post-lipopolysaccharide (LPS) (200 µg) was associated 24 post-LPS decreased clinical severity scores, number inflammatory cells, protein levels and tumor necrosis factor (TNF)-α, nitric oxide (NO) prostaglandin (PG) E2 aqueous humor (AqH) degrees histological ocular tissue...

The commercially available rebamipide ophthalmic suspension (CA-REB) was approved for clinical use in patients with dry eye; however, the residence time on ocular surface traditional formulations is short, since drug removed from through nasolacrimal duct. In this study, we designed a novel sustained-release delivery system (DDS) eye therapy by nanoparticles. solid nanoparticle-based formulation (REB-NPs) prepared bead mill using additives (2-hydroxypropyl-β-cyclodextrin and methylcellulose)...

An epidemiological study on canine intestinal parasites was undertaken to evaluate changes in the prevalence among private household dogs from Hachinohe region of Aomori prefecture, Japan, 1997, 2002 and 2007, using formalin-ethyl acetate sedimentation technique.The risk zoonotic transmission humans also discussed.All detected present (Giardia intestinalis, Isospora spp., Toxocara canis, Ancylostoma caninum, Trichuris vulpis Strongyloides stercoralis) showed no over past 10 years based...

Collagen-I is thought to be the main component of extracellular matrix in cardiac fibrosis, accumulation which occurs with excessive activation metalloproteinase-2 (MMP-2). MMP-2 degrades matrix; however, relative importance collagen-I synthesis fibroblasts remains unclear. We investigated whether regulates and phosphorylation focal adhesion kinase (FAK) rat fibroblasts. Primary cultures were incubated purified active determine affects FAK Exogenous significantly stimulated (Tyr397) induced...

A natural case of salpingitis, apparently caused by Mycoplasma gallisepticum (MG), in layer chickens is described. In the flock from which originated, there was a 3 to 4% drop egg production per month around 250 days old. The reduced 70% at 400 age, 77% predisease level. Salpingitis characterized marked thickening oviductal mucosa due epithelial hyperplasia and lymphoplasmacytic infiltration. Colonization MG on surface evidenced electron microscopy immunohistochemistry using rabbit...

Our objective was to investigate the effects of doxycycline, a matrix metalloproteinase (MMP) inhibitor (MMPi) on β-agonist-induced myocardial fibrosis and MMP expression. Twenly-four Wistar-Kyoto rats were divided into 3 groups: control (CTL; n=8), isoproterenol (ISO; with doxycycline (ISO+DOX; n=8). ISO ISO+DOX received L-isoproterenol (2.0 mg/kg/d) for 14 d, whereas CTL group vehicle. In addition, subcutaneous injection (25 injected saline. Cardiac evaluated via histopathological...

We designed a 0.05% mometasone furoate (MF) nanocrystal dispersion and investigated whether the application of MF nanocrystals in nasal formulations enhanced local absorption compared to traditional (CA-MF).MF dispersions (MF-NPs) were prepared by bead milling microcrystal (MF-MPs) consisting MF, 2-hydroxypropyl-β-cyclodextrin, methylcellulose, purified water. Pluronic F-127 combined with F-68, or carbopol was used as base for situ gelation (thickener). concentrations measured using...

Two kinds of selective agar were developed for the isolation Streptococcus iniae, causal agent streptococcosis, from Japanese flounder (Paralichthys olivaceus) and culture tanks in farms. The agars heart infusion with added thallium acetate oxlinic acid (TAOA), colistin sulphate oxolinic (CSOA). For samples containing various bacterial flora, supplemented defibrinated horse blood order to distinguish beta-haemolytic colonies Strep. iniae. iniae was quantitatively isolated brain kidney...

Objective: To investigate the anti-inflammatory effects of instillation disulfirum (DSF) eyedrops that enhance solubility using 2-hydroxypropyl-β-cyclodextrin (HPβCD) and hydroxypropylmethylcellulose (HPMC) on endotoxin-induced uveitis (EIU) in rats mechanisms related to ocular inflammation.Methods: EIU was induced male Lewis by subcutaneous injection 200 μg lipopolysaccharide (LPS). DSF (0.125%, 0.25% 0.5%) or commercially available 0.05% dexamethasone (Dexa) topically applied both eyes 1...

Background: The clinical implications of evaluating C‐terminal atrial natriuretic peptide (ANP) concentration in cats are still controversial. Hypothesis: objective this study was to investigate the relationship between plasma ANP and left pressure (LAP) healthy with volume overload (study 1), compare normal cardiomyopathy 2). Animals: Five adult were used 1, clinically (n=8) (n=14) 2. Methods: In anesthetized given acetated Ringer's solution (100 mL/kg/h for 60 minute) via cephalic vein....

To determine whether measurement of plasma atrial natriuretic peptide (ANP) concentration could be used to identify heart disease in dogs and assess severity affected dogs.Cross-sectional study.37 healthy 78 with disease.Dogs were divided into 5 groups on the basis ANP concentration: healthy, ANP-1 (< 50 pg/mL; n = 19), ANP-2 (50 100 24), ANP-3 (101 200 20), ANP-4 (> 15). All underwent physical examination, echocardiography, thoracic radiography, blood sampling before treatment.Compared...

Kidney regeneration is required for dogs with end-stage renal failure. Decellularization one of the bioengineering techniques, which involves removal all tissue cells and cellular components conservation extracellular matrix (ECM). Studies in rats have shown that decellularized kidney has regenerative potential; however, there are no reports on decellularization dogs. Here, we showed canine kidney.The artery cadaveric was cannulated whole frozen at -80°C. After completely thawing, it...

1. Single atrial myocytes were enzymatically isolated from the bull‐frog as previously described (Hume &amp; Giles, 1981), and patch‐clamp techniques used in an attempt to identify separate two inwardly rectifying K+ channels this tissue. 2. Single‐channel measurements consistently demonstrated existence of different resting channels, which both exhibited strong inward rectification. The unitary conductances these 34 +/‐ 4 22 3 pS (mean S.D., at 22‐24 degrees C) when measured with 110 mM‐K+...

Purpose: Disulfiram (DSF) exhibits a wide variety of biological activities, including an anti-inflammatory action, on which we focused our attention. The aim the present study was to investigate effect oral DSF endotoxin-induced uveitis (EIU) in rats.Methods: We investigated its upon cellular infiltration and protein leakage, as well concentration tumor necrosis factor-α (TNF-α), nitric oxide (NO), prostaglandin E2 (PGE2) anterior chamber. Some eyes were enucleated for histologic examination...

We previously found the instillation of sericin to be useful as therapy for keratopathy with or without diabetes mellitus. In this study, we investigated whether a combination solid magnesium hydroxide nanoparticles (MHN) enhances epithelial corneal wound healing by using rabbits, normal rats and type 2 mellitus debrided epithelium (ex vivo in studies). Ophthalmic formulations containing MHN (N-Ser) were prepared bead mill method. The mean particle size N-Ser was 110.3 nm at time...