A5089471264- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Tissue Engineering and Regenerative Medicine
- Liver Disease Diagnosis and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Corneal Surgery and Treatments
- Diabetes and associated disorders
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Aortic Disease and Treatment Approaches
- Ocular Infections and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Aortic aneurysm repair treatments
- Influenza Virus Research Studies
- interferon and immune responses
- Infectious Aortic and Vascular Conditions
- Chronic Kidney Disease and Diabetes
- Immune Response and Inflammation
- Cardiac Valve Diseases and Treatments
- RNA Interference and Gene Delivery
- Diabetes Management and Research
- Diet, Metabolism, and Disease
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
Kitasato University
2019-2025
Sophia University
2020-2024
Innovation Research Center
2023
Oji Holdings (Japan)
2023
University of Oxford
2023
Keio University
2015-2022
Tokyo Medical University
2012-2021
Yokohama City University
2011-2021
Meiji University
2017
Keio University Hospital
2016
One of the final treatments for end-stage heart failure is transplantation. However, a shortage donor hearts has created long waiting list and limited benefits. Our ultimate goal to create whole beating fabricated on an organ scaffold human Here, we successfully performed first transplantation using decellularized porcine with mesenchymal stem cells. A was harvested following cardiac arrest induced by high-potassium solution stored at −80°C 24 h. The completely 1% sodium dodecyl sulphate...
Abstract The resectable liver volume is strictly limited and this reduces the number of patients who may be treated. Recently, “tissue/organ decellularization”, a new approach in bioengineering, has been investigated for its ability to produce native organ scaffold by removing all viable cells. Such support repair damaged or injured tissue. purpose study was evaluate potential contribution scaffolds hepatic regeneration after hepatectomy. We sutured partial onto surfaces partially...
The derangement of endoplasmic reticulum (ER) homeostasis triggers β-cell apoptosis, leading to diabetes. Glucokinase upregulates insulin receptor substrate 2 (IRS-2) expression in β-cells, but the role glucokinase and IRS-2 ER stress has been unclear. In this study, we investigated impact activation by activator (GKA) on β-cells. GKA administration improved apoptosis Akita mice, a model stress-mediated increased even under stress. Both glucokinase-deficient mice IRS-2-deficient exhibited an...
The prevalence of nonalcoholic fatty liver disease (NAFLD) and steatohepatitis (NASH) is increasing with the growing epidemics obesity diabetes. NAFLD encompasses a clinicopathologic spectrum ranging from isolated hepatic steatosis to NASH, which more aggressive form disease, cirrhosis and, finally, hepatocellular carcinoma (HCC). exact mechanism behind development HCC in NASH remains unclear; however, it has been established that important risk factor HCC. Metformin recently drawn attention...
This study investigated the anti-inflammatory effects of water-dispersible hesperetin (WD-Hpt) in an endotoxin-induced uveitis (EIU) rat model. The rats were orally administered 10, 25, or 50 mg/kg WD-Hpt immediately after lipopolysaccharide (LPS) injection at concentration 200 μg. Clinical scores, cellular inflammation, aqueous humor (ApH) protein concentration, as well levels tumor necrosis factor (TNF)-α, cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) AqH, histopathological...
A sublingual vaccine comprising the Poly(I:C) adjuvant and influenza HA antigen was evaluated for safety in both mice macaque monkeys relative to its intranasal counterpart. Safety assessed terms of harmful effects corresponding upregulation inflammation-associated genes Saa3, Tnf, IL6, IL1b, Ccl2, Timp1, C2, Ifi47, Aif1, Omp, Nos2, and/or Gzmb SAA2, TNF, IL1B, CCL2, TIMP, AIF1, GZMB macaques. Quantitative gene expression analyses were performed using RT-qPCR with RNA samples from four...
Chronic exposure to high glucose and fatty acid levels caused by dietary sugar fat intake induces β cell apoptosis, leading the exacerbation of type 2 diabetes. Oleic linoleic are two major acids, but their effects in diabetes unclear. We challenged cell-specific glucokinase haploinsufficient (Gck(+/-)) mice with a diet containing sucrose oleic (SO) or (SL) analyzed apoptosis. In Gck(+/-) not wild-type mice, SL significantly decreased mass proportion islet cells arising from increased...
Chronic low-grade inflammation in the pancreatic islets is observed individuals with type 2 diabetes, and macrophage levels are elevated of these individuals. However, molecular mechanisms underlying interactions between β cells macrophages their involvement not fully understood. Here, we investigated role S100 calcium-binding protein A8 (S100A8), a member damage-associated pattern molecules (DAMPs), β-cell inflammation. Co-cultivation unstimulated peritoneal presence palmitate (to induce...
Abstract Lymphatic vessels play a crucial role in systemic immune response and regulation of tissue fluid homeostasis. Corneal lymphangiogenesis bacterial keratitis has not been studied. In this study, we investigated the mechanism corneal murine model using Pseudomonas aeruginosa . We first demonstrated that was enhanced mainly late stage keratitis, contrary to angiogenesis started earlier. Corresponding delayed lymphangiogenesis, expression pro-lymphangiogenic factors VEGF-C VEGFR-3...
It has not been considered that nephrons regenerate in adult mammals. We present an organ-derived extracellular matrix situ induces nephron regeneration a preclinical model. A porcine kidney-derived was sutured onto the surface of partial nephrectomy (PN)-treated kidney. Twenty-eight days after implantation, glomeruli, vessels, and renal tubules, characteristic nephrons, were histologically observed within matrix. No fibrillogenesis nor matrix-sutured kidney, although this occurred PN kidney...
To compare the efficacy and safety of these two agents impact on surrogate markers related to diabetic complications in Japanese type 2 patients.In a multicenter, open-label trial, 130 patients whose diabetes had been inadequately controlled (HbA1c, 6.9-9.5%) with metformin and/or sulphonylurea were randomly assigned sitagliptin group (50 mg/day) or pioglitazone (15 followed up for 24 weeks. At 16 weeks, if patient's HbA1c level was ≥6.5%, dose increased 100 30 mg/day, respectively. Main...
Metformin has been widely used for the treatment of type 2 diabetes. However, effect metformin on pancreatic β-cells remains controversial. In this study, we investigated impacts with in a mouse model fed high-fat diet (HFD), which triggers adaptive β-cell replication. An 8-wk improved insulin resistance and suppressed compensatory hyperplasia induced by HFD-feeding. contrast, increment mass arising from 60 wk HFD feeding was similar mice treated those without metformin. Interestingly,...
The glucokinase-induced up-regulation of insulin receptor substrate 2 (IRS-2) plays an important role in β-cell adaptive proliferation response to high-fat diet-induced resistance. This study aimed investigate the IRS-2 β-cells after a 60% partial pancreatectomy. IRS-2-deficient (IRS-2−/−) mice or wild-type were subjected pancreatectomy (60% pancreatectomy) sham operation (Sham). and gene expression profiles islets then assessed. Gene from pancreatectomized Sham C57BL/6J male was analyzed...
Objective We assessed the effect of canagliflozin, an sodium-glucose co-transporter type-2 inhibitor, on hepatic steatosis using three imaging modalities: magnetic resonance (MRI), computed tomography, and transient elastography. further determined factors associated with improving by canagliflozin among patients type 2 diabetes non-alcoholic fatty liver disease (NAFLD).
Various immune mediators are hypothesized to have important roles in the pathogenesis of vitreoretinal B-cell lymphoma, although exact mechanisms remain unclear. We determined mediator profile vitreous eyes with lymphoma.We studied 28 (23 patients) and 27 (27 undergoing vitrectomy for macular hole epiretinal membrane served as controls. Undiluted samples were collected, cytometric bead array ELISA used determine concentrations 38 mediators, including 14 interleukins (IL); interferon (IFN)-γ;...
(Pro)renin receptor ((P)RR), a for renin and prorenin, is implicated in the pathophysiology of diabetes mellitus, hypertension their complications. Soluble (P)RR (s(P)RR) composed extracellular domain thus exists blood. We have reported that plasma concentrations s(P)RR were elevated male patients with obstructive sleep apnea syndrome (OSAS). The aim present study was to clarify difference between female OSAS patients. Plasma studied 289 subjects (206 males 83 females) consisting 259 30...
Diazoxide is a non-diuretic benzothiadiazine derivative, one of group substances introduced into clinical practice in the 1950s for treatment hypertension. Fajans reported use diazoxide insulinoma 1979. Although patients with hyperinsulinemic hypoglycemia worldwide have been treated more than 30 years, there are no recent reports about adverse effects this drug Asian patients, including Japanese patients. Herein, we report results our retrospective record review 6 (3 females and 3 males,...
Decreased β-cell mass is a hallmark of type 2 diabetes, and therapeutic approaches to increase the pancreatic have been expected. In recent years, gastrointestinal incretin peptides shown exert cell-proliferative effect in β-cells. Trefoil factor (TFF2), which predominantly expressed surface epithelium stomach, plays role antiapoptosis, migration, proliferation. The TFF family β-cells, whereas TFF2 β-cells has obscure. this study, we investigated mechanism by enhances effects on cell...
The glucagon-like peptide-1 receptor agonist liraglutide is used to treat diabetes. A hallmark of the glucose-dependent facilitation insulin secretion from pancreatic β-cells. In β-cells, glycolytic enzyme glucokinase plays a pivotal role as glucose sensor. However, in action remains unknown. We first examined effects on haploinsufficient (Gck+/−) mice. Single administration significantly improved tolerance Gck+/− mice without increase secretion. also assessed survival rates, metabolic...