A5057511184- Advanced Materials Characterization Techniques
- 3D Printing in Biomedical Research
- Diamond and Carbon-based Materials Research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Ion-surface interactions and analysis
- Angiogenesis and VEGF in Cancer
- Allergic Rhinitis and Sensitization
- Pluripotent Stem Cells Research
- Electronic and Structural Properties of Oxides
- Congenital heart defects research
- Force Microscopy Techniques and Applications
- Biomedical Ethics and Regulation
- Monoclonal and Polyclonal Antibodies Research
- Bone Tissue Engineering Materials
- Indoor Air Quality and Microbial Exposure
- Liver physiology and pathology
- PARP inhibition in cancer therapy
- Thermal Radiation and Cooling Technologies
- Diagnosis and Treatment of Venous Diseases
- Extracellular vesicles in disease
- Microbial Inactivation Methods
- Advancements in Photolithography Techniques
- Axon Guidance and Neuronal Signaling
- Pancreatic and Hepatic Oncology Research
Yamagata University
2022-2025
Keio University
2015-2024
TU Wien
2024
Ehime University
2021
Korea University
2019
Massachusetts Institute of Technology
2019
The University of Tokyo
2013
Advanced Cell Technology (United States)
2012
Akita Prefectural University
2009
Medical & Biological Laboratories (Japan)
2000-2006
We have cloned a cardiovascular-restricted basic helix-loop-helix factor that interacts with arylhydrocarbon receptor nuclear translocator (ARNT) in yeast two-hybrid screen. Cardiovascular 1 (CHF1) is distantly related to the hairy family of transcriptional repressors. analyzed its expression pattern during mouse embryo development. At day 8.5, CHF1 first detected primitive ventricle primordial heart tube and persists throughout gestation. In rat hearts, this down-regulated after birth,...
Hemodynamic and biochemical factors play important roles in critical steps of angiogenesis. In particular, interstitial flow has attracted attention as an hemodynamic factor controlling the angiogenic process. Here, we applied a wide range magnitudes to vitro three-dimensional (3D) angiogenesis model microfluidic device. This study aimed investigate effect magnitude combination with vascular endothelial growth (VEGF) concentration on 3D microvascular network formation. Human umbilical vein...
Vascular endothelial growth factor‐A (VEGF‐A) is one of the major angiogenic factors, and its actions are primarily mediated through two membrane receptors, VEGFR‐1 VEGFR‐2. A soluble form (sVEGFR‐1) sequesters free VEGF‐A, acts as a potent anti‐angiogenic factor. While sVEGFR‐1 synthesized splice variant VEGF‐R1 gene, interactions between VEGF‐A remain largely unknown. Here, we show that upregulates sVEGF‐R1 expression in human vascular cells but leaves full‐length unchanged, this induction...
Construction of capillary networks is a fundamental challenge for the development three-dimensional (3D) tissue engineering. However, it not well understood how to construct stable that maintain luminal size similar structures in vivo (i.e., <10 μm diameter). In this study, we demonstrated construction covered by pericyte-like perivascular cells using an vitro 3D angiogenesis model optimizing interactions between endothelial and cells. Our can be combined with culture epithelial context...
We have devised a new method of counting pollen allergen particles, modified from the fluorescent immunoblotting technique Schumacher et al. Airborne Japanese cedar allergens collected on Burkard's sampling tape were transferred onto nitrocellulose membrane. The membrane was then treated with antiallergen mouse monoclonal antibody conjugated alkaline phosphatase. Pollen detected as purple spots after phosphate substrate staining performed. particles visible under stereoscopic microscope or...
Vascular networks consist of hierarchical structures various diameters and are necessary for efficient blood distribution. Recent advances in vascular tissue engineering bioprinting have allowed us to construct large vessels, such as arteries, small capillaries microvessels, intermediate-scale arterioles, individually. However, little is known about the control vessel between vessels vessels. Here, we focus on remodeling, which creates lasting structural changes wall response hemodynamic...
We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These have been termed TIGA, Transcript Induced by Growth Arrest. found there two kinds G0 phases caused starvation, namely, shallow (or G0/G1) and deep phases. The is induced only a few hours while generated 3 days starvation. propose mammalian cells enter through gate, which opened on third day TIGA1, one uncharacterized TIGA genes, encodes homolog cyanate permease bacteria...
During the preimplantation development of mouse embryos between 4-cell to 8-cell stage and morula stage, when first irreversible segregation cell fates proceeds into pluripotent inner mass (progenitor cells form fetus) trophectoderm (to placenta) blastocysts, pluripotency-maintaining differentiation-inducing genes are expressed coordinately regulate fates. Three structurally related cDNAs (Crxos1, Crxos1 sv2, tv3) that exhibited concomitant elevated expression during this critical period...
Japanese cedar ( Cryptomeria japonica ) pollinosis has been reported to occur naturally in monkeys Macaca fuscata as well humans. Using monoclonal antibodies (mAb) specific Cry j 1, a major allergen pollen, we identified five independent epitopes (EP‐1 EP‐5) on the molecule. The recognized by IgE sera of humans and with were analysed an enzyme‐linked immunosorbent assay inhibition method these mAb. In human patients, mAb EP‐1 strongly blocked binding all patients’ 1. reaction patterns...
The homeoprotein EGAM1C was identified in preimplantation mouse embryos and embryonic stem (ES) cells. To explore the impact of on hallmarks ES cells, MG1.19 cells stably expressing at levels similar to those blastocysts were established using an episomal expression system. In presence leukemia inhibitory factor (+LIF), control transfectants with empty vector formed flattened cell colonies, while Egam1c compacted colonies increased E-CADHERIN expression. transfectants, cellular contents...
Reconstruction of the biliary system is indispensable for regeneration transplantable liver grafts. Here, we report establishment first continuous three-dimensional scaffold bile acid excretion using a novel method. We confirmed preservation liver-derived extracellular matrix distribution in scaffold. In addition, hepatocyte progenitors decellularized via duct by slow-speed perfusion differentiated into hepatocyte- and cholangiocyte-like cells, mimicking hepatic cords ducts, respectively....
Diamonds grown by chemical vapor deposition and high-pressure high-temperature processes were mass analyzed a newly developed scanning atom probe. The analysis of the diamonds revealed that contain large amount hydrogen not only in surface layer but also deep underlying layers carbon atoms form clusters, C3, C5, C8, C16. clustering could be strongly bound diamond structure. detection number neutral particles indicates most cluster ions decomposed into positive ion particle immediately after...
After obtaining the Fowler–Nordheim plot (F–N plot) of various carbon specimens including artificial diamonds and nanotubes (CNTs), surface composition is analyzed by scanning atom probe (SAP). Since emitters vary from a sharp slender CNT to dull corner small diamond grain, slope F–N hardly related work function. Accordingly, intercept are plotted on Sasaki–Kaneko (S–K) chart. Even when slopes plots in wide range, points corresponding intercepts aligned S–K chart as suggested analytical...
Carbon nanotubes, chemical vapor deposition (CVD) diamond, high-temperature-high-pressure (HPHT) vitreous carbon, graphite and silicon are analyzed by a scanning atom probe (SAP). All specimens contain large amount of hydrogen. The CVD diamond grown in hydrogen exhibits the highest concentration with ratio to carbon atoms (H/C) 2.02, has lowest H/C 0.25. Significant difference is noticed desorption energy among CNTs. [111]-oriented wafers chemically etched HF NH4F. Although few areas...
Formation of vascular anastomoses is critical for the development transplantable tissue-engineered grafts, because rapid blood perfusion required maintenance implanted tissue grafts. However, process anastomosis remains unclear due to difficulties in observing after transplantation. Although several groups have developed vitro models anastomosis, there a lack suitable model that includes perivascular cells. Therefore, we aimed establish an containing cells by combination human umbilical vein...
Abstract Background and Aims The detailed mechanism of interstitial fibrosis in CKD has not been clarified. In this study, we focused on semaphorin class 3C (SEMA3C), a secreted protein expressed the renal tubules, that is involved embryonic kidney development [1], investigated relationship between SEMA3C tubular damage fibrosis. Methods &lt; Clinical research&gt; We correlation serum concentration marker urinary β2 microglobulin (β2MG) 191 patients who underwent biopsy from 2017 to...