A5069544169- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Immune cells in cancer
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- RNA Interference and Gene Delivery
- Infectious Diseases and Tuberculosis
- Disaster Management and Resilience
- Renal and related cancers
- Aortic Disease and Treatment Approaches
- Coronary Artery Anomalies
- Neurogenesis and neuroplasticity mechanisms
- Prostate Cancer Treatment and Research
- Kawasaki Disease and Coronary Complications
- Cardiac Valve Diseases and Treatments
- Redox biology and oxidative stress
- Viral Infections and Outbreaks Research
- Disaster Response and Management
- Pancreatic and Hepatic Oncology Research
- Aortic aneurysm repair treatments
- vaccines and immunoinformatics approaches
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Proteomics Techniques and Applications
Kanazawa Medical University
2019-2025
Kanazawa Medical University Hospital
2020-2025
Developmental toxicity testing is essential to identify substances that may harm embryonic development. This study aimed establish a protocol for evaluating developmental using human induced pluripotent stem cells (iPSCs) by analyzing cellular activity and gene expression changes. Two ICH S5(R3) positive substances, valproic acid (VPA), which substance previously detected as other test methods, thalidomide (Thalido), were examined during early trichoderm differentiation without fetal bovine...
Objectives: Prostate cancer stem cells (CSCs) play an important role in cell survival, proliferation, metastasis, and recurrence; thus, removing CSCs is for complete removal. However, the mechanisms underlying CSC functions remain largely unknown, making it difficult to develop new anticancer drugs targeting CSCs. Herein, we aimed identify novel factors that regulate stemness predict prognosis. Methods: We reanalyzed 2 single-cell RNA sequencing data of prostate (PCa) tissues using Seurat....
Given the recent advancements of immune checkpoint inhibitors, there is considerable interest in cancer immunotherapy provided through dendritic cell (DC)-based vaccination. Although many studies have been conducted to determine potency DC vaccines against cancer, clinical outcomes are not yet optimal, and further improvement necessary. In this study, we evaluated potential ability human platelet lysate (HPL) produce interferon-α-induced DCs (IFN-DCs). presence HPL, IFN-DCs (HPL-IFN-DCs)...
Significant recent advances in cancer immunotherapeutics include the vaccination of patients with tumor antigen-associated peptide-pulsed dendritic cells (DCs). DC vaccines homogeneous, mature, and functional activities are required to achieve effective acquired immunity; however, yield autologous monocyte-derived DCs varies each patient. Priming a low dose recombinant human granulocyte colony-stimulating factor (rhG-CSF) 16-18 h prior apheresis resulted 50% more harvested monocytes,...
With recent advances in cancer vaccination therapy targeting tumor-associated antigens (TAAs), dendritic cells (DCs) are considered to play a central role as cell-based drug delivery system the bioactive immune environment. Ex vivo generation of monocyte-derived DCs has been conventionally applied adherent manufacturing systems with separate loading TAAs before clinical use. We developed pre-pulsed Wilms' tumor (WT1) peptides low-adhesion culture maturation (WT1-DCs). Quality tests...
BACKGROUND Septic arthritis of the facet joint (SAFJ) has been considered a rare type spinal infection. However, because aging population, increase in compromised hosts, and MRI use recent years, number reports increasing. We report clinical progress case septic cervical (SACFJ) with some imaging considerations, we compare our findings existing SACFJ. CASE REPORT A 73-year-old Japanese woman presented fever, paralytic symptoms, paresthesia upper limbs. Here, SACFJ which allowed early...
Abstract As the sentinels of innate and adaptive immune system, dendritic cells (DCs) have been considered to hold a great promise for medical application. Among diverse types DCs, monocyte‐derived DCs (mo‐DCs) generated in vitro most commonly employed. We improving culture protocol devised produce mature interferon‐α‐induced (IFN‐DCs), hereinafter called (mat)IFN‐DCs. While exploring relationship between expression CD56 cytotoxic activity (mat)IFN‐DCs, we unexpectedly found that sorting...
Human dendritic cell (DC) dexosomes were evaluated for their function and preclinical validation vaccines. Dexosomes are small DC-secreted vesicles that contain absorbing immune signals. Vaccine manufacturing requires a significant number of monocyte-derived DCs (Mo-DCs) from donor blood; thus, Mo-DC expected to serve as novel materials cancer vaccination. In this study, we characterized potential dexosome model using immature mature MUTZ3-derived (M-imIL-4-DC, M-imIFN-DC, M-mIL-4-DC,...
Knee osteoarthritis (Knee OA) is an irreversible condition that causes bone deformity and degeneration of the articular cartilage comprises joints, resulting in chronic pain movement disorders. The administration cultured adipose-derived stem cells (ADSCs) into knee joint cavity improves clinical symptoms OA; however, effect synovial fluid (SF) filling on injected ADSCs remains unclear. In this study, we investigated adding SF from OA patients to prepared for therapeutic use environment...
Significant recent advances in cancer immunotherapeutics include the vaccination of patients with tumor antigen-associated peptide-pulsed dendritic cells (DCs). DC vaccines homogeneous, mature, and functional activities are required to achieve effective acquired immunity; however, yield autologous monocyte-derived DCs varies each patient. Priming a low dose recombinant human granulocyte colony-stimulating factor (rhG-CSF) 16–18 h prior apheresis resulted 50% more harvested...
Administration of adipose-derived stem cells (ADSCs) into the joint cavity has been shown to alleviate symptoms knee osteoarthritis (OA) by releasing exosomes and anti-inflammatory cytokines. However, therapeutic effect these is limited their rapid disappearance after administration. Thus, it necessary prolong cell survival in cavity. This study aimed investigate potential application ADSCs adhered atelocollagen microspheres (AMSs) for therapy OA. were cultured 2, 4, 7 days AMS suspension or...
A 68-year-old man underwent endovascular abdominal aortic aneurysm repair for a two-humped (AAA) with short neck. The aorta had severe calcification, suggesting high risk type Ia endoleak. Initially, catheter was placed in the sac, followed by stent graft deployment. Then, coils were inserted into Subsequently, endoleak resolved. One year after surgery, no evidence of observed, and size decreased 10 mm. Therefore, this procedure may be effective short-neck AAAs.
<title>Abstract</title> Background Adipose-derived stem cells (ADSCs) are widely used in the field of regenerative medicine because their various functions, including anti-inflammatory effects. ADSCs considered to exert effects by secreting cytokines and extracellular vesicles. Although recent studies have reported that metabolites a variety physiological activities, whether those secreted properties remains unclear. Here, we performed multiomics analyses examine effect ADSC-derived on...
This study aimed to evaluate the impact of long-term exposure physical barriers used as preventive measures during coronavirus disease 2019 (COVID-19) pandemic on cognitive function and behavior in an apolipoprotein E
Adipose-derived stem cells (ADSCs) are widely used in the field of regenerative medicine because their various functions, including anti-inflammatory effects. ADSCs considered to exert effects by secreting cytokines and extracellular vesicles. Although recent studies have reported that metabolites a variety physiological activities, whether those secreted properties remains unclear. Here, we performed multiomics analyses examine effect ADSC-derived on M1-like macrophages, which play an...
Introduction: Earthquakes are among the most striking natural phenomena, with a very high potential to set off chain of events, including casualties and injuries, substantially impacting public health. In particular, earthquakes can trigger outbreaks epidemics infectious diseases during post-earthquake period. Accordingly, we analyzed in Himi City Noto earthquake January 2024, period which COVID-19 infections were also prevalent. Materials methods: A cross-sectional study was conducted from...
Dendritic cell (DC) vaccines for cancer immunotherapy have been actively developed to improve clinical efficacy. In our previous report, monocyte−derived DCs induced by interleukin (IL)−4 with a low−adherence dish (low−adherent IL-4−DCs: la−IL-4−DCs) improved the yield and viability, as well relatively prolonged survival in vitro, compared IL-4−DCs using an adherent culture protocol. However, la−IL-4−DCs exhibit remarkable cluster formation display heterogeneous immature phenotypes....
Malignant pleural effusion (MPE) provides a liquid tumor microenvironment model that includes cancer cells and immune cells. However, the characteristics of antigen-specific CD8+ T have not been investigated in detail. Here, we analyzed MPE samples taken from patient with pancreatic who received dendritic cell vaccine targeting Wilms' Tumor 1 (WT1) antigen over disease course (two points at MPE1st 2nd, two months after MPE1st). Epithelial adhesion molecule (EpCAM)+ (PD-L1- or immunoglobulin...