A5081180297- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Bacteriophages and microbial interactions
- Weed Control and Herbicide Applications
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Cellular transport and secretion
- Advanced NMR Techniques and Applications
- SARS-CoV-2 detection and testing
- Legume Nitrogen Fixing Symbiosis
- Genomics, phytochemicals, and oxidative stress
- Crystallography and molecular interactions
- Agriculture and Biological Studies
- Dermatological and Skeletal Disorders
- Dermatologic Treatments and Research
- Glutathione Transferases and Polymorphisms
- Viral gastroenteritis research and epidemiology
- Genetic and rare skin diseases.
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- Connexins and lens biology
- Retinal Development and Disorders
- Macrophage Migration Inhibitory Factor
- Polyamine Metabolism and Applications
Durham University
2011-2023
St Vincents Institute of Medical Research
2011
National Institute of Biomedical Innovation, Health and Nutrition
2011
With current drug treatments failing due to toxicity, low efficacy and resistance; leishmaniasis is a major global health challenge that desperately needs new validated targets. Inspired by activity of the natural chalcone 2’,6’-dihydroxy-4’-methoxychalcone (DMC), nitro-analogue, 3-nitro-2’,4’,6’- trimethoxychalcone (NAT22, 1c ) was identified as potent broad spectrum antileishmanial lead. Structural modification provided an alkyne containing chemical probe labelled protein within parasite...
The Virus-X-Viral Metagenomics for Innovation Value-project was a scientific expedition to explore and exploit uncharted territory of genetic diversity in extreme natural environments such as geothermal hot springs deep-sea ocean ecosystems. Specifically, the project set analyse viral metagenomes with ultimate goal developing new gene products high innovation value applications biotechnology, pharmaceutical, medical, life science sectors. Viral pool analysis is also essential obtain...
The Horizon2020 Virus-X project was established in 2015 to explore the virosphere of selected extreme biotopes and discover novel viral proteins. To evaluate potential biotechnical value these proteins, analysis protein structures functions is a central challenge this program. stability sample essential provide meaningful assay results increase crystallizability targets. thermal shift (TSA), fluorescence-based technique, as popular method for optimizing conditions high-throughput. In TSAs,...
This work presents an updated solid-form discovery approach to the polymorphism of antiarrhythmic drug mexiletine hydrochloride, in which experimental and computational techniques are combined provide a rigorous characterization landscape this compound. The resulting solid forms were characterized by powder single-crystal X-ray diffraction, IR spectroscopy, differential scanning calorimetry, 13C solid-state NMR. reveals five types hydrochloride. Forms 1, 2, 3 mutually enantiotropically...
The Horizon2020 Virus-X project was established in 2015 to explore the virosphere of selected extreme biotopes and discover novel viral proteins. To evaluate potential biotechnical value these proteins, analysis protein structures functions is a central challenge this program. stability sample essential provide meaningful assay results increase crystallizability targets. thermal shift (TSA), fluorescence-based technique, as popular method for optimizing conditions high-throughput. In TSAs,...
As part of the Virus-X Consortium that aims to identify and characterize novel proteins enzymes from bacteriophages archaeal viruses, genes putative lytic XepA Bacillus subtilis prophage PBSX YomS SPβ were cloned subsequently produced functionally characterized. In order elucidate role molecular mechanism YomS, crystal structures these solved at resolutions 1.9 1.3 Å, respectively. consists two antiparallel β-sandwich domains connected by a 30-amino-acid linker region. A pentamer this...
The evolution and growth of multiple-herbicide resistance (MHR) in grass weeds continues to threaten global cereal production. While various processes can contribute resistance, earlier work has identified the phi class glutathione-
BFSP1 (beaded filament structural protein 1) is a plasma membrane, Aquaporin 0 (AQP0/MIP)-associated intermediate expressed in the eye lens. myristoylated, post-translation modification that requires caspase cleavage at D433. Bioinformatic analyses suggested sequences 434-452 were α-helical and amphipathic.
Bacteriophages encode a wide variety of cell wall disrupting enzymes that aid the viral escape in final stages infection. These lytic have accumulated notable interest due to their potential as novel antibacterials for infection treatment caused by multiple-drug resistant bacteria. Here, detailed functional and structural characterization Thermus parvatiensis prophage peptidoglycan amidase AmiP, globular Amidase_3 type enzyme adapted high temperatures is presented. The sequence structure...
Chagas disease is a neglected tropical (NTD) caused by Trypanosoma cruzi , whilst leishmaniasis, which over 20 species of Leishmania represents group NTDs endemic to most countries in the and subtropical belt planet. These diseases remain significant health problem both globally. parasites other trypanosomatids, including T. theileri bovine pathogen, rely on cysteine biosynthesis for production trypanothione, essential parasite survival hosts. The de novo pathway requires conversion O...
Sessions C308for both prokaryotic and eukaryotic enzymes is largely conserved.However there are several structural differences between UGM AfUGM mainly because of five additional inserts in AfUGM, two with play an important role tetramerization.Comparing the un-complexed, native structure ligandbound (both oxidized reduced FAD) allowed us to address changes (loop movements) that substrate binding redox state.Structural studies on revealed a unique mechanism significantly different from...
Eukaryotic Rab5s are highly conserved small GTPase-family proteins that involved in the regulation of early endocytosis. Leishmania donovani Rab5a regulates sorting endosomes uptake essential nutrients through fluid-phase Here, 1.80 Å resolution crystal structure N-terminal GTPase domain L. complex with GDP is presented. The determination was enabled by design specific single-site mutations and two deletions were made to stabilize protein for previous NMR studies. LdRab5a shows canonical...