A5056671566- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Genetic Neurodegenerative Diseases
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Cellular transport and secretion
- Bacterial Genetics and Biotechnology
- Fungal Biology and Applications
- Fermentation and Sensory Analysis
- Heat shock proteins research
- Arsenic contamination and mitigation
- Genetics, Aging, and Longevity in Model Organisms
University of Gothenburg
2011-2023
Chalmers University of Technology
2020
Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail the yeast Saccharomyces cerevisiae. Yeast accumulate glycerol as compatible solute, controlled at different levels by High Osmolarity Glycerol (HOG) response pathway. Up now, essentially all osmostress studies have performed with glucose carbon energy source, which metabolised glycolysis by-product. Here we investigated stress when respiring ethanol source. Remarkably, do not...
Abstract Mitogen-activated protein kinases (MAPKs) have a number of targets which they regulate at transcriptional and post-translational levels to mediate specific responses. The yeast Hog1 MAPK is essential for cell survival under hyperosmotic conditions it plays multiple roles in gene expression, metabolic regulation, signal fidelity cycle regulation. Here we describe non-essential using engineered cells osmoadaptation was reconstituted Hog1-independent manner. We rewired Hog1-dependent...
Signal transmission progresses via a series of transient protein-protein interactions and protein movements, which require diffusion within cell packed with different molecules. Yeast Hog1, the effector kinase High Osmolarity Glycerol pathway, translocates transiently from cytosol to nucleus during adaptation high external osmolarity. We followed dynamics osmostress-induced volume loss Hog1 nuclear accumulation upon exposure cells NaCl concentrations. While peaked five minutes following mild...
Spatial sorting to discrete quality control sites in the cell is a process harnessing toxicity of aberrant proteins. We show that yeast t-snare phosphoprotein syntaxin5 (Sed5) acts as key factor mitigating proteotoxicity and spatial deposition clearance IPOD (insoluble protein deposit) inclusions associates with disaggregase Hsp104. Sed5 phosphorylation promotes dynamic movement COPII-associated Hsp104 boosts disaggregation by favoring anterograde ER-to-Golgi trafficking. Hsp104-associated...
Abstract Adaptation is an important property of living organisms enabling them to cope with environmental stress and maintaining homeostasis. mediated by signaling pathways responding different stimuli. Those might communicate in order orchestrate the cellular response multiple simultaneous stimuli, a phenomenon called crosstalk. Here, we investigate possible mechanisms crosstalk between High Osmolarity Glycerol (HOG) Cell Wall Integrity (CWI) yeast, which mediate adaptation hyper-...
The accumulation of misfolded proteins is a hallmark aging and many neurodegenerative diseases, making it important to understand how the cellular machinery recognizes processes such proteins. A key question in this respect whether are handled similar way regardless their genetic origin. To approach question, we compared three different proteins, guk1-7, gus1-3, pro3-1, by cell. We show that all nontoxic, even though highly overexpressed, highlighting usefulness analyzing response misfolding...
Protein overexpression based on introduction of multiple gene copies is well established. To improve purification or quantification, proteins are typically fused to peptide tags. In Saccharomyces cerevisiae, this has been hampered by multicopy toxicity the TAP and GFP cassettes used in global strain collections. Here, we show that effect due EF-1α promoter HIS3MX marker cassette rather than tags per se. This frequently heterologous cassettes, including HIS3MX, KanMX, NatMX, PatMX HphMX....
Abstract ER-to-Golgi trafficking partakes in the sorting of misfolded cytoplasmic proteins to reduce their cytological toxicity. We show here that yeast Sec7, a protein involved proliferation Golgi, is part this pathway and participates an Hsp70-dependent formation insoluble deposits (IPOD). Sec7 associates with disaggregase Hsp104 during mild heat shock increases rate diffusion manner when overproduced. overproduction increased IPODs from smaller aggregates mitigated toxicity Huntingtin...
Central to proteopathies and leading most age-related neurodegenerative disorders is a failure in protein quality control (PQC). To harness the toxicity of misfolded damaged disease proteins, such proteins are either refolded, degraded by temporal PQC, or sequestered spatial PQC into specific, organelle-associated, compartments within cell. Here, we discuss impact vesicle trafficking pathways general, syntaxin 5 particular, as key players directing surface vacuole mitochondria, which...