A5003380738- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Wnt/β-catenin signaling in development and cancer
- Cytomegalovirus and herpesvirus research
- Reproductive System and Pregnancy
- Genetic and Kidney Cyst Diseases
- HIV/AIDS drug development and treatment
- Diabetic Foot Ulcer Assessment and Management
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Plant Pathogenic Bacteria Studies
- Wound Healing and Treatments
- Renal and related cancers
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Microbial infections and disease research
- Diet and metabolism studies
- Pressure Ulcer Prevention and Management
- Tryptophan and brain disorders
- Bone health and treatments
- Hedgehog Signaling Pathway Studies
Rush University Medical Center
2014-2025
Rush University
2020
Arkansas Agricultural Experiment Station
2009
HIV invades the brain during acute infection. Yet, it is unknown whether long-lived infected cells release productive virus that can egress from to re-seed peripheral organs. This understanding has significant implication for as a reservoir and most importantly interplay between Given sheer number of astrocytes in human their controversial role infection, we evaluated infection vivo support We developed two novel models chimeric astrocyte/human blood mononuclear cells: NOD/scid-IL-2Rgc null...
Significance The level of IL-12 p40 monomer (p40), a neglected member the family cytokines, is less in serum MS patients as compared to controls. Accordingly, supplementation inhibits disease process experimental autoimmune encephalomyelitis (EAE), an animal model MS. internalization IL-12Rβ1, but neither IL-12Rβ2 nor IL-23R, and suppresses EAE via IL-12Rβ1. These results describe anti-autoimmune property that may be beneficial treatment other disorders.
Persistent inflammation is a major contributor to healing impairment in diabetic chronic wounds. Paradoxically, wound environment during the acute phase of completely different because it exhibits reduced macrophage response owing inadequate expression CCL2 proinflammatory cytokine. What causes reduction wounds early after injury remains unknown. In this study, we report that contrast prolonged exposure high glucose, which makes monocytes proinflammatory, short-term glucose rapid monocyte...
During HIV infection, IL-10/IL-10 receptor and programmed death-1 (PD-1)/programmed death-1-ligand (PD-L1) interactions have been implicated in the impairment of cytotoxic T lymphocyte (CTL) activity. Despite antiretroviral therapy (ART), attenuated anti-HIV CTL functions present a major hurdle towards curative measures requiring viral eradication. Therefore, deeper understanding mechanisms underlying impaired is crucial before eradication viable. The generation robust activity necessitates...
Abstract Background The Wnt/β-catenin signaling pathway is a prolific regulator of cell-to-cell communication and gene expression. Canonical involves partnering β-catenin with members the TCF/LEF family transcription factors (TCF1, TCF3, TCF4, LEF1) to regulate IL-6 key cytokine involved in inflammation particularly hallmark brain. Astrocytes, specialized glial cells brain, secrete IL-6. How astrocytes expression not entirely clear, although other NFκB C/EBP pathways play role. We evaluated...
Latency is the main obstacle towards an HIV cure, with cure strategies aiming to either elicit or prevent viral reactivation. While these have shown promise, they only succeeded in modulating latency a fraction of latent reservoir, suggesting that mechanisms controlling are not completely understood, and comprehensive modulation will require targeting multiple maintenance pathways. We show here transcriptional co-activator central mediator canonical Wnt signaling, β-catenin, inhibits...
Combination antiretroviral therapy (cART) dramatically changed the face of HIV/AIDS pandemic, making it one most prominent medical breakthroughs past 3 decades. However, as life span persons living with HIV (PLWH) continues to approach that general population, same cannot be said regarding their quality life.
Abstract CD8+ T cells do not rely solely on cytotoxic functions for significant HIV control. Moreover, the noncytotoxic cell antiviral response is a primary mediator of natural control such as that seen in elite controllers and long-term nonprogressors does require combined antiretroviral therapy. In this study, we investigated biological factors contributing to replication mediated by human cells. We report canonical Wnt signaling inhibits transcription an MHC-independent, manner mediators...
Summary The variation of macrophage functions suggests the involvement multiple signalling pathways in fine tuning their differentiation. Macrophages that originate from monocytes blood migrate to tissue response homeostatic or ‘danger’ signals and undergo substantial morphological functional modifications meet needs dominant microenvironment. Wnts are secreted glycoproteins play a significant role organ cell differentiation, yet impact on monocyte differentiation is not clear. In this...
Bone mineral density (BMD) loss in people living with HIV occurs the initiation of combined antiretroviral therapy (cART), particularly tenofovir disoproxil fumarate (TDF) containing cART. Switching from TDF to abacavir (ABC) or dolutegravir (DTG) leads increased BMD. Whether BMD gains are due cessation anabolic effects ABC DTG is unclear. We investigated and on osteoblast lineage cells
HIV anti-retrovirals (ARVs) have vastly improved the life expectancy of people living with (PLWH). However, toxic effects attributed to long-term ARV use also contribute HIV-related co-morbidities such as heart disease, bone loss and HIV-associated neurocognitive disorders (HAND). Unfortunately, mouse models used study ARVs on viral suppression, toxicity latency/tissue reservoirs not been widely established. Here, we demonstrate an effective model utilizing immune-compromised mice,...
CD4dimCD8bright T cells, a genuine population of CD8+ are highly activated and cytolytic. Recently, the low affinity IgG Fc fragment receptor CD32a was described as marker HIV latency while others reported that is associated with cell activation. Given we have previously established cells activated, mediate anti-HIV responses, infected by HIV, assessed here CD32 expression on in context HIV. frequency peripheral CD4+ determined flow cytometry among negative positive patients. We report HIV-...
The shipping and receiving period is one of the most stressful experiences during a calf's lifetime. Stressors include weaning, commingling, transportation, processing, feed water changes, disease challenge placed on animal upon entering stocker operation or feedlot. These stressors result in decreased appetite, loss body mass, immunity, increased risk disease. Bovine respiratory complex has highest treatment costs all diseases affecting feedlot cattle can negatively affect performance...